##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062080_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1767735
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23661691373424	32.0	32.0	32.0	32.0	32.0
2	30.741965283257954	32.0	32.0	32.0	32.0	32.0
3	30.76645368225441	32.0	32.0	32.0	32.0	32.0
4	30.776683722390516	32.0	32.0	32.0	32.0	32.0
5	30.683442936865536	32.0	32.0	32.0	32.0	32.0
6	34.32772672374536	36.0	36.0	36.0	32.0	36.0
7	34.26377369911214	36.0	36.0	36.0	32.0	36.0
8	34.21732273219685	36.0	36.0	36.0	32.0	36.0
9	34.36821865268267	36.0	36.0	36.0	32.0	36.0
10	34.02884255841515	36.0	36.0	36.0	32.0	36.0
11	34.347597914845835	36.0	36.0	36.0	32.0	36.0
12	34.14609316441661	36.0	36.0	36.0	32.0	36.0
13	34.2505488662045	36.0	36.0	36.0	32.0	36.0
14	34.13481885011045	36.0	36.0	36.0	32.0	36.0
15	34.08823664180434	36.0	36.0	36.0	32.0	36.0
16	34.09242844657146	36.0	36.0	36.0	32.0	36.0
17	34.01633785607005	36.0	36.0	36.0	32.0	36.0
18	34.034121064526076	36.0	36.0	36.0	32.0	36.0
19	34.032100399663975	36.0	36.0	36.0	32.0	36.0
20	34.01039805174418	36.0	36.0	36.0	32.0	36.0
21	33.99741363948782	36.0	36.0	36.0	32.0	36.0
22	33.96521820295463	36.0	36.0	36.0	32.0	36.0
23	33.92448132780083	36.0	36.0	36.0	32.0	36.0
24	33.90550110734924	36.0	36.0	36.0	32.0	36.0
25	33.539739836570526	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	19.0
5	132.0
6	380.0
7	93.0
8	335.0
9	240.0
10	134.0
11	44.0
12	77.0
13	63.0
14	236.0
15	327.0
16	554.0
17	757.0
18	1172.0
19	1441.0
20	2309.0
21	3457.0
22	5165.0
23	7866.0
24	10967.0
25	15452.0
26	21671.0
27	27617.0
28	36974.0
29	48993.0
30	64407.0
31	87823.0
32	123866.0
33	175189.0
34	373493.0
35	756480.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.21922368707625	17.376613311534946	11.796245435491581	25.60791756589722
2	16.73068481123793	19.337703538100882	37.38917978331508	26.542431867346107
3	19.257522359183813	22.946434127383707	27.958794612299386	29.83724890113309
4	12.646798909679704	15.48094145921426	35.57277458001283	36.29948505109321
5	14.992168029716217	36.3500754902338	33.23160449002782	15.426151990022161
6	34.95642821764664	35.24471803580273	16.359159414137125	13.439694332413502
7	30.849049231128177	30.057638486969896	20.087108980798874	19.006203301103046
8	28.320843307515137	32.982106218736	18.906348308339123	19.790702165409733
9	26.955442684675663	14.500497271238238	18.477194619955643	40.066865424130455
10	16.14702700253202	26.42441613467846	30.826163057454725	26.6023938053348
11	37.44356438560097	21.25916140500746	21.92064062140639	19.376633587985175
12	24.891341967065838	23.493529232168783	28.297876799661946	23.31725200110343
13	29.008675596578243	19.48336105876602	25.12116778729524	26.386795557360493
14	23.554627745461115	19.912249453045757	24.667339115030014	31.865783686463118
15	25.14102900334595	27.487839983414688	21.77355265073351	25.597578362505857
16	25.8867792874049	26.001808985003095	23.480387464877992	24.63102426271401
17	24.037572725789786	26.231750584525038	24.861005383067805	24.869671306617366
18	24.864561494122032	25.091944141456356	26.00238086338579	24.041113501035824
19	25.534922041557074	25.01408546408419	25.133903924394325	24.31708856996441
20	25.780354217709302	24.489762407217302	24.554425060118714	25.175458314954685
21	26.795837570579884	24.30054289603439	24.310904804075005	24.592714729310725
22	25.96166914322992	24.47601433386489	24.97678221948391	24.58553430342128
23	24.108494083237225	24.616915017271022	25.608165987545384	25.66642491194636
24	24.62260000441652	25.088033103504507	25.465418943580108	24.823947948498866
25	24.71614731519779	24.70623872898318	25.10569630466355	25.471917651155486
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	332.0
1	332.0
2	425.0
3	518.0
4	518.0
5	518.0
6	1141.5
7	1765.0
8	1765.0
9	1765.0
10	2201.0
11	2637.0
12	2637.0
13	2637.0
14	3708.5
15	4780.0
16	4780.0
17	4780.0
18	7898.0
19	11016.0
20	11016.0
21	11016.0
22	17718.0
23	24420.0
24	24420.0
25	24420.0
26	36486.5
27	48553.0
28	48553.0
29	48553.0
30	60793.0
31	73033.0
32	73033.0
33	73033.0
34	91321.0
35	109609.0
36	109609.0
37	109609.0
38	126566.5
39	143524.0
40	143524.0
41	143524.0
42	164418.5
43	185313.0
44	185313.0
45	185313.0
46	206113.0
47	226913.0
48	226913.0
49	226913.0
50	233287.5
51	239662.0
52	239662.0
53	239662.0
54	223155.5
55	206649.0
56	206649.0
57	206649.0
58	191402.0
59	176155.0
60	176155.0
61	176155.0
62	155957.0
63	135759.0
64	135759.0
65	135759.0
66	110974.0
67	86189.0
68	86189.0
69	86189.0
70	64752.5
71	43316.0
72	43316.0
73	43316.0
74	33171.5
75	23027.0
76	23027.0
77	23027.0
78	18761.0
79	14495.0
80	14495.0
81	14495.0
82	9978.5
83	5462.0
84	5462.0
85	5462.0
86	4070.0
87	2678.0
88	2678.0
89	2678.0
90	1806.0
91	934.0
92	934.0
93	934.0
94	572.0
95	210.0
96	210.0
97	210.0
98	498.0
99	786.0
100	786.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011313913001665973
2	3.9598695505830905E-4
3	2.8284782504164934E-4
4	0.010239091266507705
5	0.03501656074015619
6	0.06279221715924616
7	0.10753874308083507
8	0.10895298220604333
9	0.1187395169524844
10	0.13265562994453356
11	0.13853886470539983
12	0.1320333647294419
13	0.12490559953839235
14	0.11585446913705956
15	0.13112825168930864
16	0.11902236477752605
17	0.12439647345331738
18	0.1125168648015681
19	0.09786534746441067
20	0.09311350400371096
21	0.09339635182875262
22	0.10448398657038527
23	0.08383609534234486
24	0.09266094748364433
25	0.08994560836324449
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1767735.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.90393050643822
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.20515500471821	33.39801642041464
2	15.763598771991107	14.78749482665692
3	5.732997261086513	8.067003153828077
4	2.5705147691173607	4.822689843858151
5	1.4118464517641534	3.3110573929653624
6	0.816271265931888	2.297179843900298
7	0.5103514414269409	1.6756241979784874
8	0.36756638480488446	1.3792246535512827
9	0.2546552556505491	1.0749899172719353
>10	1.177442110336893	9.669855960803737
>50	0.09033130911856688	2.9910557851434163
>100	0.08048309741992017	8.128803623024677
>500	0.012726593848229344	4.00759916323341
>1k	0.006060282784871117	4.389405217369586
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3057	0.1729331602304644	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2706	0.15307724291254063	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2377	0.1344658560248001	No Hit
TCGTAGTTCCGACCATAAACGATGC	2320	0.1312413908193253	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2237	0.12654611692363393	No Hit
ATCAGATACCGTCGTAGTTCCGACC	2139	0.12100229955281758	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2127	0.12032346477271762	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2123	0.1200971865126843	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	2017	0.11410081262180134	No Hit
GAACTACGACGGTATCTGATCGTCT	1967	0.11127233437138485	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1947	0.11014094307121826	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1908	0.10793473003589339	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1840	0.10408799961532697	No Hit
ACCATACTCCCCCCGGAACCCAAAG	1824	0.10318288657519367	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.656956500832987E-5
2	0.0	0.0	0.0	0.0	5.656956500832987E-5
3	0.0	0.0	0.0	0.0	5.656956500832987E-5
4	0.0	0.0	0.0	0.0	5.656956500832987E-5
5	0.0	0.0	0.0	0.0	5.656956500832987E-5
6	0.0	0.0	0.0	0.0	2.2627826003331948E-4
7	0.0	0.0	0.0	1.1313913001665974E-4	2.8284782504164934E-4
8	0.0	0.0	0.0	1.1313913001665974E-4	2.8284782504164934E-4
9	0.0	0.0	0.0	1.1313913001665974E-4	2.8284782504164934E-4
10	0.0	0.0	0.0	1.1313913001665974E-4	2.8284782504164934E-4
11	0.0	0.0	0.0	1.1313913001665974E-4	2.8284782504164934E-4
12	0.0	0.0	0.0	1.1313913001665974E-4	0.0010182521701499376
13	0.0	0.0	0.0	1.1313913001665974E-4	0.001244530430183257
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCACCG	80	4.311005E-10	16.626339	5
CGTTACA	45	6.7777414E-4	14.773527	2
CGAACGA	125	0.0	14.439936	16
GGTATCA	750	0.0	14.18138	1
CGCATCG	315	0.0	13.572137	13
GCGTTAT	225	0.0	13.506077	1
GCATCGC	330	0.0	13.243115	14
ATAACGA	140	3.6379788E-12	12.893896	12
CGCGTAA	185	0.0	12.838509	10
CGCCAGT	360	0.0	12.666611	18
GTATCAA	2170	0.0	12.516071	1
TCGCGTA	190	0.0	12.501361	9
ATCGCCA	350	0.0	12.485659	16
TCGCCAG	370	0.0	12.324269	17
CGTTATT	240	0.0	12.2673025	2
GCGTAAC	180	0.0	12.139523	11
CCGCGCG	55	0.0030662278	12.092226	9
ACGCCCC	260	0.0	12.057638	17
TACTATA	150	1.4551915E-11	12.029872	2
ACCGTCG	380	0.0	11.75128	8
>>END_MODULE