FastQCFastQC Report
Thu 2 Feb 2017
SRR4062080_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062080_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1767735
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT28040.15862106028335696No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT24970.14125420382579967No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT23720.13418300819975845No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA22930.1297140125641004No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA22580.12773407778880885No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG22300.1261501299685756No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC22150.12530158649345066No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC20340.11506249522694297No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA19260.10895298220604333No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA18500.10465369526541025No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC17790.10063725614981883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23300.027.7658161
GGTATCA8900.026.2080611
ACCGTAT450.001398736124.4436448
TAGGACG4650.022.235834
CGCAATA5050.021.78331236
ATACGAA5200.021.57865740
TACGAAT5500.020.4016441
CTAGCGG5650.020.24712429
TAGCGGC5700.020.06951730
AGGACGT5700.020.0695175
CAATACG5600.020.03732538
CGAATGC5500.020.00160843
AATACGA5550.019.82141339
TCTAGCG5850.019.55491328
GCGAAAG8100.019.28386518
TCAACGC33500.019.1754914
TCCTACA5800.018.9648952
CGGTCCA6150.018.95872710
AGCGAAA8250.018.93271317
ATCAACG34150.018.7460923
Produced by FastQC (version 0.11.5)