##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062080_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1767735
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27808014210275	32.0	32.0	32.0	32.0	32.0
2	31.418304214149746	32.0	32.0	32.0	32.0	32.0
3	31.56381640913372	32.0	32.0	32.0	32.0	32.0
4	31.644183093054107	32.0	32.0	32.0	32.0	32.0
5	31.615037321770515	32.0	32.0	32.0	32.0	32.0
6	35.25579399627207	36.0	36.0	36.0	36.0	36.0
7	35.26717522705609	36.0	36.0	36.0	36.0	36.0
8	35.212195266824494	36.0	36.0	36.0	36.0	36.0
9	35.3018614215366	36.0	36.0	36.0	36.0	36.0
10	35.16952993519956	36.0	36.0	36.0	36.0	36.0
11	35.301492022277095	36.0	36.0	36.0	36.0	36.0
12	35.21746245902242	36.0	36.0	36.0	36.0	36.0
13	35.2614803689467	36.0	36.0	36.0	36.0	36.0
14	35.2213815984862	36.0	36.0	36.0	36.0	36.0
15	35.19263916820112	36.0	36.0	36.0	36.0	36.0
16	35.20568241280509	36.0	36.0	36.0	36.0	36.0
17	35.1843788803186	36.0	36.0	36.0	36.0	36.0
18	35.1829974515411	36.0	36.0	36.0	36.0	36.0
19	35.17130395675822	36.0	36.0	36.0	36.0	36.0
20	35.17829142942805	36.0	36.0	36.0	36.0	36.0
21	35.16292261000659	36.0	36.0	36.0	36.0	36.0
22	35.1497899854899	36.0	36.0	36.0	36.0	36.0
23	35.11692589669832	36.0	36.0	36.0	36.0	36.0
24	35.100731727323385	36.0	36.0	36.0	36.0	36.0
25	35.07748503027886	36.0	36.0	36.0	36.0	36.0
26	35.04201421593169	36.0	36.0	36.0	36.0	36.0
27	35.02495905777732	36.0	36.0	36.0	36.0	36.0
28	35.00214794638337	36.0	36.0	36.0	36.0	36.0
29	34.9796247740753	36.0	36.0	36.0	36.0	36.0
30	34.959857105278786	36.0	36.0	36.0	36.0	36.0
31	34.96663809903634	36.0	36.0	36.0	36.0	36.0
32	34.938155888750295	36.0	36.0	36.0	36.0	36.0
33	34.91971647334018	36.0	36.0	36.0	32.0	36.0
34	34.90783856177538	36.0	36.0	36.0	32.0	36.0
35	34.89295963478689	36.0	36.0	36.0	32.0	36.0
36	34.868964522397306	36.0	36.0	36.0	32.0	36.0
37	34.854480450972574	36.0	36.0	36.0	32.0	36.0
38	34.83199518027306	36.0	36.0	36.0	32.0	36.0
39	34.81970035101415	36.0	36.0	36.0	32.0	36.0
40	34.80754920845036	36.0	36.0	36.0	32.0	36.0
41	34.77986236624834	36.0	36.0	36.0	32.0	36.0
42	34.73956051104945	36.0	36.0	36.0	32.0	36.0
43	34.74471173563911	36.0	36.0	36.0	32.0	36.0
44	34.690169058145024	36.0	36.0	36.0	32.0	36.0
45	34.67967653522728	36.0	36.0	36.0	32.0	36.0
46	34.6536324731931	36.0	36.0	36.0	32.0	36.0
47	34.63652527103893	36.0	36.0	36.0	32.0	36.0
48	34.609750330224834	36.0	36.0	36.0	32.0	36.0
49	34.59117345077175	36.0	36.0	36.0	32.0	36.0
50	34.17088138210761	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	13.0
21	37.0
22	116.0
23	305.0
24	805.0
25	1947.0
26	4051.0
27	7504.0
28	13305.0
29	21301.0
30	32750.0
31	49453.0
32	75890.0
33	130714.0
34	302243.0
35	1127299.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.90017966528499	17.86489736443759	12.1912091332211	26.04371383705632
2	16.142859729039234	19.70818652709042	37.240642304184796	26.908311439685544
3	18.62516490056185	23.468432997762104	28.2745348276775	29.631867273998548
4	12.377816810777635	15.916865367263759	35.74885375918902	35.95646406276959
5	14.671656102300402	36.73695435118952	33.09048019075257	15.500909355757509
6	35.094895713711935	35.3258133312214	16.269962041714514	13.309328913352154
7	30.883305472822563	30.06802490192252	20.179042673251363	18.86962695200355
8	28.223743943520947	33.18053893824583	18.85107213468082	19.744644983552398
9	27.01971732188365	14.194378682325123	18.517594548956716	40.26830944683451
10	16.23038307799703	26.431352228332994	30.85311477858094	26.485149915089035
11	37.70333223022681	21.05304245263006	21.973881831835655	19.26974348530747
12	24.881670612393826	23.375675652742068	28.422359686265192	23.320294048598914
13	29.14594099228674	19.322749167720275	25.217976676368348	26.313333163624637
14	23.64455079522666	19.789561218169013	24.66608400014708	31.899803986457247
15	25.128540194090178	27.4343156638297	21.823519928043513	25.613624214036605
16	25.97029971256874	25.855406406079673	23.566138889093168	24.60815499225842
17	24.182301080195845	26.2451668377896	24.728140812961218	24.844391269053336
18	24.95912846616063	24.989732618142774	26.046226411892288	24.004912503804306
19	25.67415364859552	24.860061038560644	25.240038806721593	24.225746506122242
20	25.931603846965405	24.336677922149917	24.592486073955822	25.139232156928852
21	26.876935739802626	24.2356461799987	24.309016905814502	24.578401174384172
22	26.049040155905722	24.38063397511505	25.062693220420478	24.50763264855875
23	24.272344450403118	24.366250087682975	25.685624291464997	25.675781170448914
24	24.792175679505625	24.951250736118126	25.502695847020934	24.753877737355314
25	24.863864956752447	24.4458146888948	25.252046409802404	25.438273944550353
26	24.512580831111556	25.621121789595573	25.61178777968795	24.25450959960492
27	25.239869640799423	24.935411553002126	24.89021236828454	24.934506437913914
28	24.717473933834285	24.7321256335163	25.555053337278167	24.99534709537125
29	24.61925746578946	24.957261572751495	25.539364043151387	24.88411691830766
30	24.536943276894966	24.956299392999984	25.818318615610025	24.688438714495025
31	25.28670999537825	24.789970397012663	24.65691817797658	25.266401429632516
32	25.090313974708696	24.786816026501903	24.60285044820396	25.52001955058544
33	24.495765756082662	24.65297302189814	25.310652644917493	25.540608577101708
34	25.36801517882296	24.813291200106804	25.461807995130464	24.356885625939768
35	25.891665643698055	24.426340885575918	25.53131669920367	24.150676771522363
36	24.316398460627255	25.209915750851515	25.161209300273292	25.312476488247942
37	25.559864343880907	24.984824956652833	24.894085834457673	24.561224865008587
38	24.67011439503907	24.590690680837728	25.389849377791002	25.3493455463322
39	25.7671026512638	24.50316028020227	24.51453083995626	25.21520622857767
40	25.668892679049744	24.75687815198545	25.205983928586583	24.368245240378226
41	24.45962909305258	25.356263799547552	25.665418920745974	24.51868818665389
42	25.915237984787755	25.342049212091727	24.963185689515203	23.779527113605315
43	25.253285255005032	24.3780658278605	25.308329434606325	25.060319482528143
44	24.68782404890497	25.11018723485622	24.817542611105107	25.3844461051337
45	24.946257970791493	25.27991111634075	24.872320252711994	24.901510660155772
46	24.6327275154679	25.198606319083687	24.91801461000298	25.25065155544543
47	25.035950466988368	24.683686803830945	25.454231746157458	24.826130983023234
48	25.827288191877116	25.447466810886404	24.12646456669305	24.59878043054343
49	24.900688701649738	25.516073665166765	24.48200290325026	25.101234729933235
50	25.063160812576722	25.715134269761442	24.39234942383083	24.829355493831002
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	164.0
1	104.0
2	44.0
3	99.0
4	154.0
5	173.5
6	193.0
7	161.5
8	130.0
9	186.0
10	242.0
11	410.5
12	579.0
13	1095.5
14	1612.0
15	2205.5
16	2799.0
17	2925.0
18	3051.0
19	3096.0
20	3141.0
21	3827.5
22	4514.0
23	5428.5
24	6343.0
25	8362.0
26	10381.0
27	13372.0
28	16363.0
29	19219.0
30	22075.0
31	25111.0
32	28147.0
33	32612.5
34	37078.0
35	43161.0
36	49244.0
37	58091.5
38	66939.0
39	72631.5
40	78324.0
41	84514.0
42	90704.0
43	93525.5
44	96347.0
45	106197.0
46	116047.0
47	123644.5
48	131242.0
49	136106.5
50	140971.0
51	134899.5
52	128828.0
53	127766.0
54	126704.0
55	128778.0
56	130852.0
57	126816.5
58	122781.0
59	112944.0
60	103107.0
61	89693.5
62	76280.0
63	64955.5
64	53631.0
65	44866.5
66	36102.0
67	31421.0
68	26740.0
69	24244.0
70	21748.0
71	16832.0
72	11916.0
73	10572.0
74	9228.0
75	6583.0
76	3938.0
77	3620.5
78	3303.0
79	2818.0
80	2333.0
81	1855.0
82	1377.0
83	1222.5
84	1068.0
85	829.5
86	591.0
87	403.0
88	215.0
89	138.0
90	61.0
91	45.0
92	29.0
93	22.5
94	16.0
95	22.5
96	29.0
97	25.5
98	22.0
99	15.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031678956404664727
2	0.005600386935824657
3	1.697086950249896E-4
4	0.0
5	0.0
6	2.8284782504164934E-4
7	0.0
8	0.0
9	0.0
10	5.656956500832986E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.525565200666389E-4
17	0.0
18	5.656956500832986E-5
19	0.0
20	2.2627826003331945E-4
21	0.0
22	0.0
23	1.697086950249896E-4
24	9.051130401332778E-4
25	2.8284782504164934E-4
26	3.394173900499792E-4
27	2.2627826003331945E-4
28	0.001244530430183257
29	2.8284782504164934E-4
30	0.0014142391252082468
31	4.525565200666389E-4
32	7.354043451082882E-4
33	2.8284782504164934E-4
34	5.091260850749688E-4
35	2.2627826003331945E-4
36	1.1313913001665973E-4
37	0.0016970869502498961
38	5.656956500832986E-5
39	6.788347800999584E-4
40	0.0
41	7.919739101166181E-4
42	0.004525565200666389
43	0.0036204521605331112
44	0.003507313030516452
45	0.001753656515258226
46	0.0023759217303498544
47	0.0014142391252082468
48	0.003507313030516452
49	0.003450743465508122
50	0.0014142391252082468
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1767735.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.791163771881976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.27325492989644	33.58440635137893
2	16.36135365978856	15.638562645692714
3	5.946369368798772	8.525517370570936
4	2.7328648839817746	5.22427172947193
5	1.430335707467293	3.4178704022170034
6	0.8281071436239889	2.374572247295967
7	0.5051558359983382	1.6899389701962975
8	0.3559716756248143	1.3609840518349399
9	0.25214900594042533	1.0845444994034499
>10	1.1472194997725536	9.542401767810391
>50	0.07695012717396474	2.5755256670867617
>100	0.07407287666391424	7.552451470362595
>500	0.010836551172790079	3.5457917885744448
>1k	0.005358734096434654	3.8831610381036774
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2804	0.15862106028335696	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2497	0.14125420382579967	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	2372	0.13418300819975845	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2293	0.1297140125641004	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2258	0.12773407778880885	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	2230	0.1261501299685756	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2215	0.12530158649345066	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2034	0.11506249522694297	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	1926	0.10895298220604333	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1850	0.10465369526541025	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1779	0.10063725614981883	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2627826003331948E-4
2	0.0	0.0	0.0	0.0	2.2627826003331948E-4
3	0.0	0.0	0.0	0.0	2.2627826003331948E-4
4	0.0	0.0	0.0	0.0	2.2627826003331948E-4
5	0.0	0.0	0.0	0.0	3.394173900499792E-4
6	0.0	0.0	0.0	0.0	3.394173900499792E-4
7	0.0	0.0	0.0	0.0	3.394173900499792E-4
8	0.0	0.0	0.0	0.0	3.394173900499792E-4
9	0.0	0.0	0.0	5.656956500832987E-5	3.394173900499792E-4
10	0.0	0.0	0.0	5.656956500832987E-5	3.394173900499792E-4
11	0.0	0.0	0.0	5.656956500832987E-5	3.394173900499792E-4
12	0.0	0.0	0.0	5.656956500832987E-5	7.919739101166182E-4
13	0.0	0.0	0.0	5.656956500832987E-5	0.0010182521701499376
14	0.0	0.0	0.0	1.1313913001665974E-4	0.0010182521701499376
15	0.0	0.0	0.0	1.697086950249896E-4	0.001301099995191587
16	0.0	0.0	0.0	3.394173900499792E-4	0.001301099995191587
17	0.0	0.0	0.0	3.959869550583091E-4	0.001301099995191587
18	0.0	0.0	0.0	5.091260850749688E-4	0.0014142391252082466
19	0.0	0.0	0.0	5.656956500832987E-4	0.0014142391252082466
20	0.0	0.0	0.0	7.919739101166182E-4	0.0014708086902165765
21	0.0	0.0	0.0	7.919739101166182E-4	0.0014708086902165765
22	0.0	0.0	0.0	0.0013576695601999168	0.0015273782552249066
23	0.0	0.0	0.0	0.001753656515258226	0.0015273782552249066
24	0.0	0.0	0.0	0.0023759217303498544	0.0015273782552249066
25	0.0	0.0	0.0	0.0027719086854081634	0.0015273782552249066
26	0.0	0.0	0.0	0.003563882595524782	0.0015839478202332364
27	0.0	0.0	0.0	0.004129578245608081	0.0015839478202332364
28	0.0	0.0	0.0	0.0050346912857413585	0.0015839478202332364
29	0.0	0.0	0.0	0.006562069540966265	0.0015839478202332364
30	0.0	0.0	0.0	0.00865514344627447	0.0015839478202332364
31	0.0	0.0	0.0	0.01278472169188255	0.0015839478202332364
32	0.0	0.0	0.0	0.01668802167745731	0.0015839478202332364
33	0.0	0.0	0.0	0.020591321663032073	0.0015839478202332364
34	0.0	0.0	0.0	0.02477746947364848	0.0016405173852415663
35	0.0	0.0	0.0	0.029585882499356522	0.0016405173852415663
36	0.0	0.0	0.0	0.03637423030035611	0.0016405173852415663
37	0.0	0.0	0.0	0.046047625916780514	0.0016405173852415663
38	0.0	0.0	0.0	0.05764438674348814	0.0016970869502498961
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2330	0.0	27.765816	1
GGTATCA	890	0.0	26.208061	1
ACCGTAT	45	0.0013987361	24.443644	8
TAGGACG	465	0.0	22.23583	4
CGCAATA	505	0.0	21.783312	36
ATACGAA	520	0.0	21.578657	40
TACGAAT	550	0.0	20.40164	41
CTAGCGG	565	0.0	20.247124	29
TAGCGGC	570	0.0	20.069517	30
AGGACGT	570	0.0	20.069517	5
CAATACG	560	0.0	20.037325	38
CGAATGC	550	0.0	20.001608	43
AATACGA	555	0.0	19.821413	39
TCTAGCG	585	0.0	19.554913	28
GCGAAAG	810	0.0	19.283865	18
TCAACGC	3350	0.0	19.175491	4
TCCTACA	580	0.0	18.964895	2
CGGTCCA	615	0.0	18.958727	10
AGCGAAA	825	0.0	18.932713	17
ATCAACG	3415	0.0	18.746092	3
>>END_MODULE