FastQCFastQC Report
Thu 2 Feb 2017
SRR4062079_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062079_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26927
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA10453.880863074237754No Hit
GTATCAACGCAGAGTACTTTTTTTT5181.9237196865599584No Hit
TATCAACGCAGAGTACTTTTTTTTT3611.340661789282133No Hit
GTACTTTTTTTTTTTTTTTTTTTTT2731.013852267241059No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2280.8467337616518735No Hit
GGTATCAACGCAGAGTACTTTTTTT2260.8393062725145765No Hit
GAGTACTTTTTTTTTTTTTTTTTTT1920.7130389571805251No Hit
GTACATGGGGTGGTATCAACGCAAA1630.6053403646897166No Hit
GTATCAACGCAGAGTACATGGGGTG1380.5124967504735024No Hit
TATCAACGCAGAGTACATGGGGTGG910.3379507557470197No Hit
ACGCAGAGTACATGGGGTGGTATCA880.326809522041074No Hit
GCAGAGTACTTTTTTTTTTTTTTTT800.29709956549188543No Hit
GTACATGGGTGGTATCAACGCAAAA740.27481709807999405No Hit
GGTATCAACGCAGAGTACATGGGGT690.2562483752367512No Hit
ATCAACGCAGAGTACTTTTTTTTTT580.21539718498161695No Hit
GTACATGGGAGTGGTATCAACGCAA470.17454599472648272No Hit
GTATCAACGCAGAGTACATGGGAAG410.1522635273145913No Hit
GTATCAACGCAGAGTACATGGGTGG410.1522635273145913No Hit
GTATCAACGCAGAGTACATGGGCAG410.1522635273145913No Hit
GTATCAACGCAGAGTACATGGGAGT390.14483603817729418No Hit
GTCCTACAGTGTGCATTTCTCATTT370.13740854903999702No Hit
CAACGCAGAGTACTTTTTTTTTTTT340.12626731533405133No Hit
GTCCTAAAGTGTGTATTTCTCATTT320.11883982619675419No Hit
GGTATCAACGCAGAGTACATGGGTG310.11512608162810563No Hit
ATCAACGCAGAGTACATGGGGTGGT300.11141233705945705No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT300.11141233705945705No Hit
GAGTACATGGGGTGGTATCAACGCA280.10398484792215991No Hit
CAAGTAGAGTGATCGAAAGATGAAA270.10027110335351133No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT270.10027110335351133No Hit
GTATCAACGCAGAGTACATGGGGTA270.10027110335351133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA2001.9723302E-88.551
GGTATCA1050.00422573978.1428571
TATCAAC3005.0484232E-86.651
AACGCAG2952.7020906E-76.44067765
TCAACGC3151.2778037E-76.33333353
ATCAACG3151.2778037E-76.33333352
CAACGCA3252.3075881E-76.13846164
AGAGTAC3758.586903E-85.82666710
GCAGAGT3653.2271782E-75.7260278
CAGAGTA3807.050621E-75.59
AGTACAT2100.0092969435.42857112
CGCAGAG3505.76196E-65.4285717
ACGCAGA3557.3961437E-65.3521136
Produced by FastQC (version 0.11.5)