##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062079_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26927
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.119545437664797	32.0	32.0	32.0	21.0	32.0
2	29.946373528428715	32.0	32.0	32.0	21.0	32.0
3	30.2965053663609	32.0	32.0	32.0	21.0	32.0
4	30.085044750622053	32.0	32.0	32.0	21.0	32.0
5	30.290637649942436	32.0	32.0	32.0	21.0	32.0
6	33.665800126267314	36.0	36.0	36.0	21.0	36.0
7	33.42856612322205	36.0	36.0	36.0	21.0	36.0
8	33.23378022059643	36.0	36.0	36.0	21.0	36.0
9	33.09031826790953	36.0	36.0	36.0	21.0	36.0
10	33.06294797043859	36.0	36.0	36.0	21.0	36.0
11	33.54298659338211	36.0	36.0	36.0	21.0	36.0
12	33.31979054480633	36.0	36.0	36.0	21.0	36.0
13	33.4099974003788	36.0	36.0	36.0	21.0	36.0
14	33.32376425149478	36.0	36.0	36.0	21.0	36.0
15	33.343707059828425	36.0	36.0	36.0	21.0	36.0
16	33.37137445686486	36.0	36.0	36.0	21.0	36.0
17	33.27225461432763	36.0	36.0	36.0	21.0	36.0
18	33.371931518550156	36.0	36.0	36.0	21.0	36.0
19	33.23883091320979	36.0	36.0	36.0	21.0	36.0
20	32.95562075240465	36.0	36.0	36.0	14.0	36.0
21	32.90630222453299	36.0	36.0	36.0	14.0	36.0
22	32.99550636907193	36.0	36.0	36.0	21.0	36.0
23	33.12155085973187	36.0	36.0	36.0	21.0	36.0
24	33.217736844059864	36.0	36.0	36.0	21.0	36.0
25	32.772830244735765	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	1.0
5	6.0
6	4.0
7	1.0
8	3.0
9	3.0
10	3.0
11	0.0
12	1.0
13	3.0
14	54.0
15	53.0
16	59.0
17	72.0
18	84.0
19	116.0
20	166.0
21	208.0
22	308.0
23	366.0
24	426.0
25	379.0
26	498.0
27	567.0
28	671.0
29	803.0
30	970.0
31	1251.0
32	1674.0
33	2259.0
34	4968.0
35	10949.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.61592453671037	22.338173580421138	12.21821963085379	19.827682252014707
2	14.338767779552123	24.19875960931407	39.443681063616445	22.01879154751736
3	18.80710094332615	32.05080591250093	26.63225135556711	22.509841788605808
4	11.266716196136702	20.018573551263003	33.26523031203566	35.449479940564636
5	12.04205989447871	42.30883555027123	29.300735676599537	16.348368878650515
6	27.287593845238977	39.38898386976882	18.285884189400132	15.037538095592062
7	26.04379670595234	37.13425289065695	18.853403725322526	17.968546678068186
8	27.483640690065435	33.923259964306965	18.222040452111838	20.371058893515766
9	27.64992378332156	17.529836041194187	19.686210358032493	35.13402981745176
10	17.311268129416142	30.3309780587579	27.46374116772034	24.894012644105615
11	32.17924879137225	26.64559315730755	21.219784306433617	19.955373744886575
12	25.207333853992335	29.216408196660343	26.962698501245864	18.613559448101455
13	29.05433044513034	25.320739280800268	25.778141385593695	19.846788888475697
14	23.185333928305816	24.08151123010561	27.517477316674103	25.21567752491447
15	23.994942169660455	30.480865781546356	25.27427572613336	20.249916322659825
16	20.34805890227577	31.191432396251674	26.974564926372157	21.485943775100402
17	18.68654940314603	30.411661894314086	26.800788367855414	24.101000334684468
18	18.894094898110964	28.81154246616094	31.042689275621004	21.251673360107095
19	22.455201130195555	27.645178080154658	30.68629637891293	19.213324410736856
20	24.650557620817846	25.802973977695164	30.066914498141262	19.479553903345725
21	24.778761061946902	25.875659998512678	28.831709675020452	20.51386926451997
22	23.872718486301626	27.445076391212226	28.991487305304638	19.690717817181515
23	21.30324882908334	28.84915619656531	30.789532376774957	19.058062597576388
24	21.63036205486581	28.36963794513419	30.59995539365103	19.40004460634897
25	20.9755372146628	29.22522120603762	30.169529333035918	19.62971224626366
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1110.0
1	1110.0
2	621.0
3	132.0
4	132.0
5	132.0
6	241.0
7	350.0
8	350.0
9	350.0
10	324.5
11	299.0
12	299.0
13	299.0
14	218.5
15	138.0
16	138.0
17	138.0
18	220.5
19	303.0
20	303.0
21	303.0
22	496.5
23	690.0
24	690.0
25	690.0
26	975.5
27	1261.0
28	1261.0
29	1261.0
30	1473.5
31	1686.0
32	1686.0
33	1686.0
34	1738.5
35	1791.0
36	1791.0
37	1791.0
38	1848.5
39	1906.0
40	1906.0
41	1906.0
42	2154.0
43	2402.0
44	2402.0
45	2402.0
46	2955.0
47	3508.0
48	3508.0
49	3508.0
50	3659.5
51	3811.0
52	3811.0
53	3811.0
54	3244.5
55	2678.0
56	2678.0
57	2678.0
58	2293.0
59	1908.0
60	1908.0
61	1908.0
62	1591.0
63	1274.0
64	1274.0
65	1274.0
66	995.0
67	716.0
68	716.0
69	716.0
70	597.0
71	478.0
72	478.0
73	478.0
74	383.0
75	288.0
76	288.0
77	288.0
78	205.5
79	123.0
80	123.0
81	123.0
82	89.5
83	56.0
84	56.0
85	56.0
86	34.5
87	13.0
88	13.0
89	13.0
90	8.0
91	3.0
92	3.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	1.5
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0037137445686485685
4	0.025996211980539977
5	0.04827867939243139
6	0.07798863594161994
7	0.11141233705945705
8	0.11512608162810563
9	0.11141233705945705
10	0.13740854903999702
11	0.13740854903999702
12	0.1411222936086456
13	0.13369480447134846
14	0.1299810599026999
15	0.1411222936086456
16	0.1299810599026999
17	0.13369480447134846
18	0.1299810599026999
19	0.10769859249080847
20	0.10027110335351133
21	0.12255357076540277
22	0.09655735878486278
23	0.09284361421621422
24	0.09284361421621422
25	0.10769859249080847
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	26927.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.7224347309392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.27677496991576	49.5487800349092
2	12.755716004813477	15.74627697106993
3	3.6161251504211798	6.6958814572733685
4	1.239470517448857	3.0601255245664203
5	0.631768953068592	1.9497158985404983
6	0.35499398315282793	1.3146655773015932
7	0.21660649819494585	0.9358636312994393
8	0.15643802647412755	0.7724588702789023
9	0.10830324909747292	0.6016266201210682
>10	0.5535499398315282	5.990269989230141
>50	0.036101083032490974	1.7083225015783414
>100	0.0421179302045728	5.871430163033386
>500	0.0060168471720818285	1.9237196865599584
>1k	0.0060168471720818285	3.880863074237754
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAA	1045	3.880863074237754	No Hit
GTATCAACGCAGAGTACTTTTTTTT	518	1.9237196865599584	No Hit
TATCAACGCAGAGTACTTTTTTTTT	361	1.340661789282133	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	273	1.013852267241059	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	228	0.8467337616518735	No Hit
GGTATCAACGCAGAGTACTTTTTTT	226	0.8393062725145765	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	192	0.7130389571805251	No Hit
GTACATGGGGTGGTATCAACGCAAA	163	0.6053403646897166	No Hit
GTATCAACGCAGAGTACATGGGGTG	138	0.5124967504735024	No Hit
TATCAACGCAGAGTACATGGGGTGG	91	0.3379507557470197	No Hit
ACGCAGAGTACATGGGGTGGTATCA	88	0.326809522041074	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	80	0.29709956549188543	No Hit
GTACATGGGTGGTATCAACGCAAAA	74	0.27481709807999405	No Hit
GGTATCAACGCAGAGTACATGGGGT	69	0.2562483752367512	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	58	0.21539718498161695	No Hit
GTACATGGGAGTGGTATCAACGCAA	47	0.17454599472648272	No Hit
GTATCAACGCAGAGTACATGGGAAG	41	0.1522635273145913	No Hit
GTATCAACGCAGAGTACATGGGTGG	41	0.1522635273145913	No Hit
GTATCAACGCAGAGTACATGGGCAG	41	0.1522635273145913	No Hit
GTATCAACGCAGAGTACATGGGAGT	39	0.14483603817729418	No Hit
GTCCTACAGTGTGCATTTCTCATTT	37	0.13740854903999702	No Hit
CAACGCAGAGTACTTTTTTTTTTTT	34	0.12626731533405133	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	32	0.11883982619675419	No Hit
GGTATCAACGCAGAGTACATGGGTG	31	0.11512608162810563	No Hit
ATCAACGCAGAGTACATGGGGTGGT	30	0.11141233705945705	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT	30	0.11141233705945705	No Hit
GAGTACATGGGGTGGTATCAACGCA	28	0.10398484792215991	No Hit
CAAGTAGAGTGATCGAAAGATGAAA	27	0.10027110335351133	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT	27	0.10027110335351133	No Hit
GTATCAACGCAGAGTACATGGGGTA	27	0.10027110335351133	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	200	1.9723302E-8	8.55	1
GGTATCA	105	0.0042257397	8.142857	1
TATCAAC	300	5.0484232E-8	6.65	1
AACGCAG	295	2.7020906E-7	6.4406776	5
TCAACGC	315	1.2778037E-7	6.3333335	3
ATCAACG	315	1.2778037E-7	6.3333335	2
CAACGCA	325	2.3075881E-7	6.1384616	4
AGAGTAC	375	8.586903E-8	5.826667	10
GCAGAGT	365	3.2271782E-7	5.726027	8
CAGAGTA	380	7.050621E-7	5.5	9
AGTACAT	210	0.009296943	5.428571	12
CGCAGAG	350	5.76196E-6	5.428571	7
ACGCAGA	355	7.3961437E-6	5.352113	6
>>END_MODULE