FastQCFastQC Report
Thu 2 Feb 2017
SRR4062079_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062079_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26927
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4031.496639061165373No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2921.0844134140453818No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2430.9024399301816021No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2340.8690162290637651No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2040.7576038920043079No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.4307943699632339No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.3342370111783711No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA800.29709956549188543No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.2673896089426969No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690.2562483752367512No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA650.24139339696215692No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA650.24139339696215692No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG480.17825973929513128No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG410.1522635273145913No Hit
ATACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.1411222936086456No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT380.1411222936086456No Hit
ATATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA360.13369480447134846No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA360.13369480447134846No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.1299810599026999No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.12626731533405133No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.11512608162810563No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG300.11141233705945705No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAT300.005649329629.33085418
ATGGGTG406.857203E-427.4976755
TGGGTGG406.857203E-427.4976756
CATGGGT450.001363709224.4423794
ATGGGGT750.001247643517.5985155
GAGTACA2600.016.92164612
AGTACAT2650.016.6023713
CATGGGA1205.9571885E-616.49860617
AACGCAG4300.014.8359566
GGTATCA2850.014.6927381
GTATCAA4250.014.5198821
GGGTGGT1000.00847713613.19888520
ACATGGG3350.013.13321816
ACGCAGA4900.013.0193087
CGCAGAG4900.013.0193088
AGAGTAC5100.012.50874711
GCAGAGT5100.012.5087479
GTACATG3551.8189894E-1212.3933214
TACATGG3551.8189894E-1212.3933215
CAGAGTA5150.012.38730210
Produced by FastQC (version 0.11.5)