##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062079_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26927
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.700115126081627	32.0	32.0	32.0	32.0	32.0
2	31.256211237791064	32.0	32.0	32.0	32.0	32.0
3	31.466594867605007	32.0	32.0	32.0	32.0	32.0
4	31.618524157908418	32.0	32.0	32.0	32.0	32.0
5	31.604857577895793	32.0	32.0	32.0	32.0	32.0
6	35.180896497938875	36.0	36.0	36.0	36.0	36.0
7	35.183161882125745	36.0	36.0	36.0	36.0	36.0
8	35.09447766182642	36.0	36.0	36.0	36.0	36.0
9	35.10836706651317	36.0	36.0	36.0	36.0	36.0
10	35.02547628774093	36.0	36.0	36.0	36.0	36.0
11	35.162550599769745	36.0	36.0	36.0	36.0	36.0
12	35.07917703420359	36.0	36.0	36.0	36.0	36.0
13	35.10996397667768	36.0	36.0	36.0	36.0	36.0
14	35.072418019088644	36.0	36.0	36.0	36.0	36.0
15	35.08263081665243	36.0	36.0	36.0	36.0	36.0
16	35.11798566494596	36.0	36.0	36.0	36.0	36.0
17	35.061648159839564	36.0	36.0	36.0	36.0	36.0
18	35.07746871170201	36.0	36.0	36.0	36.0	36.0
19	34.94269692130575	36.0	36.0	36.0	36.0	36.0
20	34.85523823671408	36.0	36.0	36.0	32.0	36.0
21	34.81550116982954	36.0	36.0	36.0	32.0	36.0
22	34.87532959483047	36.0	36.0	36.0	36.0	36.0
23	34.89356408066253	36.0	36.0	36.0	36.0	36.0
24	34.93697775467003	36.0	36.0	36.0	36.0	36.0
25	34.90804768448026	36.0	36.0	36.0	36.0	36.0
26	34.90073160768002	36.0	36.0	36.0	36.0	36.0
27	34.87306421064359	36.0	36.0	36.0	32.0	36.0
28	34.85260147807034	36.0	36.0	36.0	32.0	36.0
29	34.76867085081888	36.0	36.0	36.0	32.0	36.0
30	34.79559549894158	36.0	36.0	36.0	32.0	36.0
31	34.7342815761132	36.0	36.0	36.0	32.0	36.0
32	34.614996100568206	36.0	36.0	36.0	32.0	36.0
33	34.516507594607646	36.0	36.0	36.0	32.0	36.0
34	34.45979871504438	36.0	36.0	36.0	32.0	36.0
35	34.36517250343521	36.0	36.0	36.0	32.0	36.0
36	34.292643072009504	36.0	36.0	36.0	32.0	36.0
37	34.291120436736364	36.0	36.0	36.0	32.0	36.0
38	34.196717049801315	36.0	36.0	36.0	32.0	36.0
39	34.13302633044899	36.0	36.0	36.0	32.0	36.0
40	34.020908381921494	36.0	36.0	36.0	32.0	36.0
41	33.925019497158985	36.0	36.0	36.0	32.0	36.0
42	33.82422846956586	36.0	36.0	36.0	32.0	36.0
43	33.74256322650128	36.0	36.0	36.0	32.0	36.0
44	33.562038103019276	36.0	36.0	36.0	21.0	36.0
45	33.37130018197348	36.0	36.0	36.0	21.0	36.0
46	33.11984253723029	36.0	36.0	36.0	14.0	36.0
47	32.94648494076577	36.0	36.0	36.0	14.0	36.0
48	32.82942771196197	36.0	36.0	36.0	14.0	36.0
49	32.671704980131466	36.0	36.0	36.0	14.0	36.0
50	32.28952352657184	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	2.0
21	4.0
22	5.0
23	16.0
24	39.0
25	63.0
26	147.0
27	271.0
28	530.0
29	732.0
30	915.0
31	1231.0
32	1611.0
33	2318.0
34	4144.0
35	14897.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.57652303120357	20.089153046062407	14.179049034175334	21.155274888558694
2	14.240083197147527	22.47437230723518	40.81117218838211	22.47437230723518
3	18.421541318477253	29.567316620241414	29.5636025998143	22.447539461467038
4	10.992683923199763	16.919820254762875	36.55438778920786	35.5331080328295
5	11.59059679875218	39.477104764734285	31.217736844059868	17.71456159245367
6	27.775095628922642	37.50510639878189	18.639283990047165	16.080513982248302
7	25.505997697478367	34.59353065696141	21.112637872767113	18.787833772793107
8	27.74538567237345	31.284584246295537	20.169346752330377	20.800683329000634
9	28.38786348274966	13.919114643294833	21.547145987298993	36.14587588665651
10	17.911390054592044	26.38244141567943	29.713670293757193	25.99249823597133
11	31.856500909867417	24.195045864745424	24.001931147175696	19.94652207821146
12	26.360158948267536	26.063059382775656	29.275448434656663	18.301333234300145
13	29.048910015969103	22.590708211089243	28.58469194488803	19.77568982805363
14	23.559995543506517	20.518438741783342	31.110038251569055	24.811527463141086
15	23.50057563040814	26.49385375273889	29.79908641883611	20.20648419801686
16	19.94652207821146	27.975637835629662	29.813941397110703	22.26389868904817
17	18.661566457459056	26.360158948267536	30.411854272663124	24.56642032161028
18	18.902959854421216	24.005644891744346	36.038177294165706	21.053217959668736
19	21.591710922122775	22.549857020834107	36.29442566940246	19.56400638764066
20	23.686262858840568	21.368886248003864	35.86734504400788	19.0775058491477
21	23.961079956920564	21.201767742414678	35.22486723363167	19.61228506703309
22	22.271326178185465	23.043785048464365	35.02432502692464	19.66056374642552
23	20.648419801686043	23.879377576410292	36.15701712036246	19.315185501541205
24	20.793255839863335	23.303747168269766	36.09388346269544	19.809113529171462
25	20.455305084116315	24.01678612545029	36.00103984847922	19.526868941954174
26	19.538010175660116	23.741969027370295	36.86634233297434	19.853678463995248
27	19.3300404798158	25.416867827830803	35.59624169049653	19.65685000185687
28	18.777389883384092	24.463343979796477	36.17321547946223	20.5860506573572
29	19.05221718784818	24.74931293173884	35.95409641238951	20.244373468023472
30	19.31889924610985	24.273034500687043	36.13473465295057	20.273331600252533
31	19.200772487558492	25.18755106588427	35.519572160736836	20.092104285820398
32	18.839826196754185	25.661974969361605	35.85991755487058	19.63828127901363
33	19.24462435473688	24.919226055631892	35.96761614736138	19.86853344226984
34	19.43402532773796	24.659263935826495	35.61852415790842	20.288186578527128
35	19.289189289560664	25.364875403869725	35.158019831396	20.187915475173618
36	19.597430088758493	26.100196828462142	35.0057563040814	19.29661677869796
37	19.337467968953096	26.219036654658893	34.15159505329223	20.291900323095778
38	19.757121105210384	26.475285029895645	34.56753444498087	19.2000594199131
39	20.20648419801686	26.519849964719427	33.783934340996026	19.489731496267687
40	19.76826233891633	26.972926802094555	34.140453819586284	19.11835703940283
41	20.210948525588652	26.77337889029191	33.859466686474036	19.1562058976454
42	19.3300404798158	27.537415976529132	33.30486129164036	19.827682252014707
43	19.285475544992014	27.867939243138856	33.17859397630631	19.667991235562816
44	19.096783777761274	27.683280101017605	32.99413206566144	20.22580405555968
45	19.30033052326661	28.183607531473985	32.00505069261336	20.511011252646043
46	20.388457681880638	28.049912727002635	31.225164333197164	20.33646525791956
47	20.22876666542875	28.343298547925873	30.749805028410144	20.67812975823523
48	20.23769730733519	28.820798514391825	30.11699164345404	20.824512534818943
49	20.642525533890435	28.73909006499536	30.332404828226554	20.28597957288765
50	19.916056903019722	30.004085725959218	29.992942836979537	20.086914534041526
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	515.0
1	278.0
2	41.0
3	97.5
4	154.0
5	133.5
6	113.0
7	80.0
8	47.0
9	70.0
10	93.0
11	167.5
12	242.0
13	404.0
14	566.0
15	721.0
16	876.0
17	847.0
18	818.0
19	733.5
20	649.0
21	615.0
22	581.0
23	501.0
24	421.0
25	363.0
26	305.0
27	319.5
28	334.0
29	408.0
30	482.0
31	602.0
32	722.0
33	842.5
34	963.0
35	998.5
36	1034.0
37	1028.0
38	1022.0
39	1055.0
40	1088.0
41	1108.5
42	1129.0
43	1167.0
44	1205.0
45	1245.5
46	1286.0
47	1369.0
48	1452.0
49	1481.5
50	1511.0
51	1576.0
52	1641.0
53	1564.5
54	1488.0
55	1467.0
56	1446.0
57	1313.0
58	1180.0
59	1047.5
60	915.0
61	809.5
62	704.0
63	579.0
64	454.0
65	428.0
66	402.0
67	368.0
68	334.0
69	295.5
70	257.0
71	219.5
72	182.0
73	150.0
74	118.0
75	93.5
76	69.0
77	56.0
78	43.0
79	33.5
80	24.0
81	19.0
82	14.0
83	9.5
84	5.0
85	3.0
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025996211980539977
2	0.011141233705945705
3	0.007427489137297137
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0037137445686485685
29	0.0037137445686485685
30	0.0
31	0.0037137445686485685
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0037137445686485685
42	0.0
43	0.0
44	0.0037137445686485685
45	0.0
46	0.0
47	0.0
48	0.007427489137297137
49	0.007427489137297137
50	0.014854978274594274
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	26927.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.11750287815204
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.43059644531677	55.46848887733502
2	12.2941882019409	16.748988004605042
3	3.3529604187111546	6.851858729156608
4	1.0903936321011884	2.970995654918855
5	0.5888125613346418	2.005422067070227
6	0.32711808963035655	1.3369480447134845
7	0.20717479009922582	0.9878560552605192
8	0.13084723585214264	0.7130389571805251
9	0.08177952240758914	0.5013555167675567
>10	0.4307054846799695	5.236379841794482
>50	0.03271180896303566	1.6377613547740186
>100	0.03271180896303566	5.540906896423664
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	403	1.496639061165373	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	292	1.0844134140453818	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	243	0.9024399301816021	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	234	0.8690162290637651	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	204	0.7576038920043079	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	116	0.4307943699632339	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	90	0.3342370111783711	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	80	0.29709956549188543	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	72	0.2673896089426969	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	69	0.2562483752367512	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	65	0.24139339696215692	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	65	0.24139339696215692	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	48	0.17825973929513128	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	41	0.1522635273145913	No Hit
ATACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	38	0.1411222936086456	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT	38	0.1411222936086456	No Hit
ATATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	36	0.13369480447134846	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	36	0.13369480447134846	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35	0.1299810599026999	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34	0.12626731533405133	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31	0.11512608162810563	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	30	0.11141233705945705	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0037137445686485685	0.0
22	0.0	0.0	0.0	0.0037137445686485685	0.0
23	0.0	0.0	0.0	0.014854978274594274	0.0
24	0.0	0.0	0.0	0.018568722843242843	0.0
25	0.0	0.0	0.0	0.018568722843242843	0.0
26	0.0	0.0	0.0	0.018568722843242843	0.0
27	0.0	0.0	0.0	0.025996211980539977	0.0
28	0.0	0.0	0.0	0.033423701117837115	0.0
29	0.0	0.0	0.0	0.04085119025513425	0.0
30	0.0	0.0	0.0	0.07798863594161994	0.0
31	0.0	0.0	0.0	0.1411222936086456	0.0
32	0.0	0.0	0.0	0.17083225015783415	0.0
33	0.0	0.0	0.0	0.21168344041296838	0.0
34	0.0	0.0	0.0	0.23767965239350838	0.0
35	0.0	0.0	0.0	0.27853084264864264	0.0
36	0.0	0.0	0.0	0.326809522041074	0.0
37	0.0	0.0	0.0	0.39365692427674825	0.0
38	0.0	0.0	0.0	0.47535930478701677	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGAT	30	0.0056493296	29.330854	18
ATGGGTG	40	6.857203E-4	27.497675	5
TGGGTGG	40	6.857203E-4	27.497675	6
CATGGGT	45	0.0013637092	24.442379	4
ATGGGGT	75	0.0012476435	17.598515	5
GAGTACA	260	0.0	16.921646	12
AGTACAT	265	0.0	16.60237	13
CATGGGA	120	5.9571885E-6	16.498606	17
AACGCAG	430	0.0	14.835956	6
GGTATCA	285	0.0	14.692738	1
GTATCAA	425	0.0	14.519882	1
GGGTGGT	100	0.008477136	13.198885	20
ACATGGG	335	0.0	13.133218	16
ACGCAGA	490	0.0	13.019308	7
CGCAGAG	490	0.0	13.019308	8
AGAGTAC	510	0.0	12.508747	11
GCAGAGT	510	0.0	12.508747	9
GTACATG	355	1.8189894E-12	12.39332	14
TACATGG	355	1.8189894E-12	12.39332	15
CAGAGTA	515	0.0	12.387302	10
>>END_MODULE