FastQCFastQC Report
Thu 2 Feb 2017
SRR4062078_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062078_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218000
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20340.16699507389162563No Hit
GCGCAAGACGGACCAGAGCGAAAGC14160.11625615763546798No Hit
TATCAACGCAGAGTACTTTTTTTTT12720.10443349753694582No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACC350.002170023316.2855034
GCGTTAT1300.015.3402811
TTACGGC400.005280127314.249228518
CGTTATT1500.013.2960032
AAGACGG2650.012.9054935
CCGTCGT2150.012.8153689
CGTCGTA2150.012.81536810
CAAGACG2450.012.79575354
GTTATTC1650.012.6649413
CGCAAGA2350.012.5281492
ACCGTCG2300.012.3916538
CCTTATA853.9545976E-612.2904222
GCGTAAC1103.8044163E-812.09224411
ACCGTTC550.003067140512.0912498
GTCCAAT550.003076594812.0862821
CTTATAC802.869162E-511.8733823
GTCGTAG2250.011.82352611
ATACCGT2250.011.8225556
GGTATCA7150.011.8206481
GGACCGT658.0193323E-411.69263656
Produced by FastQC (version 0.11.5)