##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062078_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1218000
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29670853858785	32.0	32.0	32.0	32.0	32.0
2	30.906605090311988	32.0	32.0	32.0	32.0	32.0
3	30.929509031198688	32.0	32.0	32.0	32.0	32.0
4	30.929288177339902	32.0	32.0	32.0	32.0	32.0
5	30.878669950738917	32.0	32.0	32.0	32.0	32.0
6	34.527314449917895	36.0	36.0	36.0	32.0	36.0
7	34.46180952380952	36.0	36.0	36.0	32.0	36.0
8	34.43308456486043	36.0	36.0	36.0	32.0	36.0
9	34.52766748768473	36.0	36.0	36.0	32.0	36.0
10	34.28049261083744	36.0	36.0	36.0	32.0	36.0
11	34.51018637110016	36.0	36.0	36.0	32.0	36.0
12	34.36958702791461	36.0	36.0	36.0	32.0	36.0
13	34.44279802955665	36.0	36.0	36.0	32.0	36.0
14	34.357628899835795	36.0	36.0	36.0	32.0	36.0
15	34.320291461412154	36.0	36.0	36.0	32.0	36.0
16	34.32460016420361	36.0	36.0	36.0	32.0	36.0
17	34.260970443349755	36.0	36.0	36.0	32.0	36.0
18	34.25771510673235	36.0	36.0	36.0	32.0	36.0
19	34.2648472906404	36.0	36.0	36.0	32.0	36.0
20	34.25143513957307	36.0	36.0	36.0	32.0	36.0
21	34.225745484400655	36.0	36.0	36.0	32.0	36.0
22	34.20140722495895	36.0	36.0	36.0	32.0	36.0
23	34.184431034482756	36.0	36.0	36.0	32.0	36.0
24	34.16555747126437	36.0	36.0	36.0	32.0	36.0
25	33.832554187192116	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	14.0
5	85.0
6	274.0
7	53.0
8	235.0
9	174.0
10	80.0
11	17.0
12	45.0
13	52.0
14	204.0
15	283.0
16	394.0
17	494.0
18	704.0
19	986.0
20	1410.0
21	1973.0
22	3050.0
23	4499.0
24	6318.0
25	9095.0
26	12312.0
27	16316.0
28	21860.0
29	29374.0
30	39184.0
31	52771.0
32	75399.0
33	109091.0
34	247348.0
35	583906.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.25140765627078	17.333343185695686	11.729729996009794	25.68551916202374
2	16.91966400574057	19.17640191231998	37.2687289049152	26.635205177024247
3	19.83610716335342	22.267405916792487	27.669662583713674	30.22682433614041
4	13.255843360336586	15.281990478520838	34.72490676406011	36.73725939708247
5	15.487295478474833	35.76771643843042	32.96539580386958	15.779592279225163
6	35.36646180110268	34.194940770821404	16.376401655903077	14.062195772172842
7	31.238832589524208	29.861511781772155	19.585850366973233	19.313805261730405
8	28.36777014037461	32.9454997357548	18.844011496926456	19.842718626944134
9	26.770783672261583	15.00797317069161	18.742129576353385	39.47911358069342
10	16.99873475686203	26.41924682271058	30.38753928707372	26.194479133353667
11	37.085818300208665	21.530295630897943	21.66619803767777	19.717688031215623
12	24.78384508699223	23.532501954581367	28.06408889378505	23.619564064641356
13	29.37071445188965	19.475059661652868	24.847691178296063	26.306534708161415
14	23.553829398105336	19.779873826110187	24.69087396995664	31.975422805827836
15	25.210213913415213	27.115703974087047	21.89348723260823	25.780594879889506
16	25.87330384438648	26.064754570690855	23.180005606242823	24.881935978679838
17	24.239340240898883	26.118423864970357	24.72107224007024	24.921163654060525
18	24.854781981869426	25.37129536855828	25.525575385556206	24.248347264016097
19	25.525854261486447	25.199912556234395	25.03768135952044	24.23655182275872
20	25.759808292373854	24.65745657034991	24.634857291718884	24.94787784555735
21	26.406099638495505	24.402252027556194	24.483691208266528	24.707957125681776
22	25.808424130797665	24.400210730661723	24.83942471515609	24.951940423384528
23	24.527943741921487	24.63468486716523	25.309232901904167	25.528138489009116
24	24.673578604112976	24.99786330405512	25.220654946615472	25.107903145216433
25	24.916919223461814	24.6855914430909	25.135673948629616	25.261815384817666
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	805.0
1	805.0
2	603.5
3	402.0
4	402.0
5	402.0
6	824.5
7	1247.0
8	1247.0
9	1247.0
10	1518.5
11	1790.0
12	1790.0
13	1790.0
14	2389.5
15	2989.0
16	2989.0
17	2989.0
18	5001.0
19	7013.0
20	7013.0
21	7013.0
22	11032.0
23	15051.0
24	15051.0
25	15051.0
26	22395.0
27	29739.0
28	29739.0
29	29739.0
30	37957.0
31	46175.0
32	46175.0
33	46175.0
34	58280.5
35	70386.0
36	70386.0
37	70386.0
38	83304.0
39	96222.0
40	96222.0
41	96222.0
42	112594.0
43	128966.0
44	128966.0
45	128966.0
46	143244.5
47	157523.0
48	157523.0
49	157523.0
50	163886.0
51	170249.0
52	170249.0
53	170249.0
54	159973.5
55	149698.0
56	149698.0
57	149698.0
58	137724.5
59	125751.0
60	125751.0
61	125751.0
62	110207.0
63	94663.0
64	94663.0
65	94663.0
66	77036.5
67	59410.0
68	59410.0
69	59410.0
70	44857.5
71	30305.0
72	30305.0
73	30305.0
74	22500.5
75	14696.0
76	14696.0
77	14696.0
78	11729.0
79	8762.0
80	8762.0
81	8762.0
82	6117.5
83	3473.0
84	3473.0
85	3473.0
86	2528.5
87	1584.0
88	1584.0
89	1584.0
90	1037.0
91	490.0
92	490.0
93	490.0
94	310.5
95	131.0
96	131.0
97	131.0
98	305.5
99	480.0
100	480.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0014778325123152708
2	2.463054187192118E-4
3	7.389162561576354E-4
4	0.010016420361247946
5	0.03300492610837438
6	0.06395730706075534
7	0.10591133004926108
8	0.10894909688013138
9	0.11658456486042693
10	0.13407224958949096
11	0.13842364532019705
12	0.13357963875205253
13	0.12783251231527096
14	0.11699507389162563
15	0.1330049261083744
16	0.12307060755336618
17	0.1277504105090312
18	0.11338259441707717
19	0.1
20	0.09417077175697866
21	0.09367816091954023
22	0.10517241379310344
23	0.08513957307060756
24	0.09589490968801313
25	0.09129720853858785
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1218000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.68464294235412
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.38532851797135	44.6394504453595
2	14.062524023718742	16.22383354352985
3	4.102242429639558	7.099091694501992
4	1.5820698327476443	3.65044533647671
5	0.8059393727192148	2.3245162474246377
6	0.45576899916672686	1.577452318867604
7	0.3004362286348027	1.213138960302224
8	0.20242250965203912	0.9341336154218484
9	0.1596266295226117	0.8287204615292967
>10	0.7996694389866307	8.445343099714972
>50	0.07381148465131812	2.97740654408032
>100	0.06330167166778371	7.088587564888497
>500	0.006001503306313772	2.3528867031284095
>1k	8.573576151876817E-4	0.6449934647742093
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2034	0.16699507389162563	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1416	0.11625615763546798	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1272	0.10443349753694582	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	8.210180623973728E-5	0.0
10	0.0	0.0	0.0	8.210180623973728E-5	0.0
11	0.0	0.0	0.0	1.6420361247947455E-4	0.0
12	0.0	0.0	0.0	1.6420361247947455E-4	0.0
13	0.0	0.0	0.0	1.6420361247947455E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGACC	35	0.0021700233	16.285503	4
GCGTTAT	130	0.0	15.340281	1
TTACGGC	40	0.0052801273	14.2492285	18
CGTTATT	150	0.0	13.296003	2
AAGACGG	265	0.0	12.905493	5
CCGTCGT	215	0.0	12.815368	9
CGTCGTA	215	0.0	12.815368	10
CAAGACG	245	0.0	12.7957535	4
GTTATTC	165	0.0	12.664941	3
CGCAAGA	235	0.0	12.528149	2
ACCGTCG	230	0.0	12.391653	8
CCTTATA	85	3.9545976E-6	12.290422	2
GCGTAAC	110	3.8044163E-8	12.092244	11
ACCGTTC	55	0.0030671405	12.091249	8
GTCCAAT	55	0.0030765948	12.086282	1
CTTATAC	80	2.869162E-5	11.873382	3
GTCGTAG	225	0.0	11.823526	11
ATACCGT	225	0.0	11.822555	6
GGTATCA	715	0.0	11.820648	1
GGACCGT	65	8.0193323E-4	11.6926365	6
>>END_MODULE