FastQCFastQC Report
Thu 2 Feb 2017
SRR4062078_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062078_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218000
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13730.11272577996715928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16350.024.8970551
GGTATCA8000.024.2039741
CGTTGCG550.004484697219.99917816
GTATTAT1252.2837412E-819.3631781
TCGCGTG600.0074138918.33257925
CTAGATA2650.018.2634033
TACGCTC851.430844E-418.11764734
TAACGGC750.001290878217.636
CTATTCC2400.017.415954
GTATTAC1154.2662577E-617.2202191
TCGATTT1154.2746105E-617.21668430
TAGGACG1154.2746105E-617.2166844
ATCGATT1154.2746105E-617.21668429
ATCAACG23250.017.031563
CCTATTC3000.016.8659743
GTCCTAA2350.016.8538321
TCAACGC23450.016.7924884
CAACGCA24100.016.5221425
AACGCAG24100.016.5221426
GTATTAG2700.016.2989731
Produced by FastQC (version 0.11.5)