##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062078_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1218000
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.317379310344826	32.0	32.0	32.0	32.0	32.0
2	31.48040804597701	32.0	32.0	32.0	32.0	32.0
3	31.591926929392447	32.0	32.0	32.0	32.0	32.0
4	31.663452380952382	32.0	32.0	32.0	32.0	32.0
5	31.642816912972087	32.0	32.0	32.0	32.0	32.0
6	35.28821428571428	36.0	36.0	36.0	36.0	36.0
7	35.28926272577997	36.0	36.0	36.0	36.0	36.0
8	35.24440558292282	36.0	36.0	36.0	36.0	36.0
9	35.325658456486046	36.0	36.0	36.0	36.0	36.0
10	35.21011001642036	36.0	36.0	36.0	36.0	36.0
11	35.31826765188834	36.0	36.0	36.0	36.0	36.0
12	35.244851395730706	36.0	36.0	36.0	36.0	36.0
13	35.2813776683087	36.0	36.0	36.0	36.0	36.0
14	35.243802955665025	36.0	36.0	36.0	36.0	36.0
15	35.22806239737274	36.0	36.0	36.0	36.0	36.0
16	35.23607963875205	36.0	36.0	36.0	36.0	36.0
17	35.21938998357964	36.0	36.0	36.0	36.0	36.0
18	35.216820197044335	36.0	36.0	36.0	36.0	36.0
19	35.205568965517244	36.0	36.0	36.0	36.0	36.0
20	35.20351724137931	36.0	36.0	36.0	36.0	36.0
21	35.193865353037765	36.0	36.0	36.0	36.0	36.0
22	35.180871100164204	36.0	36.0	36.0	36.0	36.0
23	35.15466912972085	36.0	36.0	36.0	36.0	36.0
24	35.14177011494253	36.0	36.0	36.0	36.0	36.0
25	35.121648604269296	36.0	36.0	36.0	36.0	36.0
26	35.0839039408867	36.0	36.0	36.0	36.0	36.0
27	35.06826354679803	36.0	36.0	36.0	36.0	36.0
28	35.04846223316913	36.0	36.0	36.0	36.0	36.0
29	35.03670443349754	36.0	36.0	36.0	36.0	36.0
30	35.01719786535304	36.0	36.0	36.0	36.0	36.0
31	35.01995812807882	36.0	36.0	36.0	36.0	36.0
32	34.983865353037764	36.0	36.0	36.0	36.0	36.0
33	34.965245484400654	36.0	36.0	36.0	36.0	36.0
34	34.95752216748768	36.0	36.0	36.0	36.0	36.0
35	34.949841543513955	36.0	36.0	36.0	36.0	36.0
36	34.927096880131366	36.0	36.0	36.0	36.0	36.0
37	34.91065763546798	36.0	36.0	36.0	36.0	36.0
38	34.8941091954023	36.0	36.0	36.0	32.0	36.0
39	34.88187520525452	36.0	36.0	36.0	32.0	36.0
40	34.868910509031195	36.0	36.0	36.0	32.0	36.0
41	34.846889162561574	36.0	36.0	36.0	32.0	36.0
42	34.81661165845649	36.0	36.0	36.0	32.0	36.0
43	34.8128842364532	36.0	36.0	36.0	32.0	36.0
44	34.76179802955665	36.0	36.0	36.0	32.0	36.0
45	34.75988095238095	36.0	36.0	36.0	32.0	36.0
46	34.73581855500821	36.0	36.0	36.0	32.0	36.0
47	34.70976765188834	36.0	36.0	36.0	32.0	36.0
48	34.69386617405583	36.0	36.0	36.0	32.0	36.0
49	34.67353530377668	36.0	36.0	36.0	32.0	36.0
50	34.26891954022989	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	0.0
19	2.0
20	13.0
21	31.0
22	78.0
23	228.0
24	507.0
25	1294.0
26	2751.0
27	5195.0
28	9077.0
29	14448.0
30	21792.0
31	32431.0
32	48956.0
33	83292.0
34	191699.0
35	806204.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.87287832151293	17.991521498470846	12.056159807106699	26.07944037290953
2	16.283083390465087	19.66386002652008	37.00101400309538	27.052042579919455
3	19.019438487482716	22.758777532931145	28.090075829477275	30.131708150108867
4	12.919725648153774	15.785247594823637	34.93995885050714	36.35506790651545
5	14.968883415435139	36.212479474548445	32.89540229885057	15.923234811165846
6	35.34685607037121	34.42271662186637	16.29173446918632	13.938692838576088
7	31.29770114942529	29.830459770114942	19.624712643678162	19.24712643678161
8	28.376518883415436	33.00582922824302	18.720689655172414	19.89696223316913
9	26.87857142857143	14.8123973727422	18.660098522167488	39.64893267651888
10	16.994991789819373	26.474548440065682	30.418637110016423	26.11182266009852
11	37.30878489326765	21.405500821018062	21.63727422003284	19.648440065681445
12	24.799096880131362	23.49679802955665	28.085960591133002	23.61814449917898
13	29.460673234811164	19.348275862068963	24.98711001642036	26.203940886699506
14	23.691954022988508	19.496141215106732	24.858538587848933	31.95336617405583
15	25.345977011494252	26.985960591133008	21.9252052545156	25.742857142857144
16	25.99011486136996	25.88198589479388	23.342309871181207	24.785589372654947
17	24.316995073891626	26.065188834154352	24.701149425287355	24.916666666666668
18	25.002565683551463	25.207574062047673	25.626375719520293	24.16348453488057
19	25.672660098522165	25.0004105090312	25.12577996715928	24.20114942528736
20	25.89121803355676	24.485055780150343	24.76863635020148	24.855089836091416
21	26.57857142857143	24.291297208538587	24.529556650246306	24.600574712643677
22	25.910673234811167	24.320935960591132	24.91863711001642	24.84975369458128
23	24.61847290640394	24.34384236453202	25.56009852216749	25.477586206896554
24	24.820544061636127	24.78778509130229	25.333275314104338	25.05839553295725
25	25.020258670587857	24.28117639041804	25.443330320189624	25.25523461880448
26	24.76826651034406	25.177381826183055	25.55160370248129	24.5027479609916
27	25.355645375152815	24.729453356617462	25.020915486655547	24.893985781574173
28	24.85632042351813	24.662558251805436	25.453042230301122	25.028079094375315
29	24.676764127304196	24.91075504353052	25.40115074269538	25.011330086469908
30	24.732138898604415	25.091750124386074	25.557192142412617	24.618918834596897
31	25.30186955097443	24.756464117609873	24.810897927984808	25.130768403430892
32	25.176130201290487	24.853631923388598	24.63310484231821	25.337133033002708
33	24.64016683155514	24.724321528413498	25.199446631554316	25.436065008477048
34	25.340170822280296	24.780807083139365	25.31751055631774	24.561511538262597
35	25.801027587894232	24.468923594291617	25.36547679060228	24.364572027211867
36	24.6522156066194	25.04811181645917	25.017241435932462	25.28243114098897
37	25.548347793102828	24.86730044984429	24.937827233190774	24.64652452386211
38	24.930459770114943	24.648111658456486	25.274137931034485	25.147290640394086
39	25.582456955616113	24.594843787331065	24.73975502201583	25.08294423503699
40	25.620197044334976	24.708949096880133	25.081855500821014	24.588998357963877
41	24.69439479537239	25.145546813487435	25.4544155836692	24.70564280747098
42	25.770849515001537	25.095694212039653	24.97130405224551	24.162152220713303
43	25.35061049454533	24.371750166468654	25.09879329724528	25.178846041740734
44	24.765138913292294	25.035674582127076	24.902172003211945	25.297014501368686
45	25.0456907805611	25.12771250523412	24.95915334532049	24.867443368884288
46	24.91528916629104	24.905272474541594	24.992384851149495	25.187053508017872
47	25.03596094542803	24.778733452217917	25.32159594347399	24.863709658880055
48	25.664189827168606	25.260314462826884	24.381706966624243	24.69378874338027
49	25.056139276310336	25.351635079514562	24.49150336671448	25.100722277460623
50	25.204826676278202	25.38988703436261	24.614751790052555	24.79053449930664
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	361.0
1	212.5
2	64.0
3	161.5
4	259.0
5	249.5
6	240.0
7	188.5
8	137.0
9	180.5
10	224.0
11	407.0
12	590.0
13	1091.5
14	1593.0
15	1976.5
16	2360.0
17	2373.0
18	2386.0
19	2346.0
20	2306.0
21	2668.5
22	3031.0
23	3658.5
24	4286.0
25	5355.5
26	6425.0
27	8249.5
28	10074.0
29	11759.0
30	13444.0
31	15585.0
32	17726.0
33	20502.0
34	23278.0
35	26996.5
36	30715.0
37	36260.5
38	41806.0
39	46410.5
40	51015.0
41	55776.5
42	60538.0
43	63912.0
44	67286.0
45	73857.0
46	80428.0
47	85675.0
48	90922.0
49	94505.5
50	98089.0
51	96316.5
52	94544.0
53	93822.0
54	93100.0
55	93283.5
56	93467.0
57	89842.5
58	86218.0
59	78577.0
60	70936.0
61	62167.5
62	53399.0
63	45639.0
64	37879.0
65	31852.0
66	25825.0
67	21889.5
68	17954.0
69	15900.5
70	13847.0
71	10723.0
72	7599.0
73	6591.0
74	5583.0
75	4134.0
76	2685.0
77	2376.0
78	2067.0
79	1745.5
80	1424.0
81	1122.0
82	820.0
83	696.5
84	573.0
85	425.5
86	278.0
87	198.0
88	118.0
89	76.0
90	34.0
91	25.0
92	16.0
93	15.0
94	14.0
95	17.0
96	20.0
97	17.0
98	14.0
99	8.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.027257799671592777
2	0.00451559934318555
3	3.284072249589491E-4
4	1.6420361247947455E-4
5	0.0
6	1.6420361247947455E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	8.210180623973727E-4
17	0.0
18	8.210180623973728E-5
19	0.0
20	3.284072249589491E-4
21	0.0
22	0.0
23	0.0
24	0.0010673234811165847
25	2.463054187192118E-4
26	4.926108374384236E-4
27	2.463054187192118E-4
28	9.852216748768472E-4
29	3.284072249589491E-4
30	0.0014778325123152708
31	5.747126436781609E-4
32	7.389162561576354E-4
33	4.1050903119868636E-4
34	7.389162561576354E-4
35	1.6420361247947455E-4
36	3.284072249589491E-4
37	0.0018883415435139571
38	0.0
39	0.0010673234811165847
40	0.0
41	5.747126436781609E-4
42	0.004761904761904762
43	0.004351395730706076
44	0.0037766830870279143
45	0.0024630541871921183
46	0.00270935960591133
47	9.852216748768472E-4
48	0.004105090311986864
49	0.0038587848932676518
50	0.0015599343185550081
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1218000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.96872779099306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.10313108225394	45.46673548622689
2	14.357761471359007	16.933178557851544
3	4.181371319275089	7.397104413581948
4	1.6259888125375888	3.835299667109165
5	0.7923828296518417	2.3362903693998134
6	0.4398573830897408	1.5562698174166487
7	0.269248129041305	1.1114053740769623
8	0.1998080947624924	0.9425943320389087
9	0.14824053167480614	0.7867400003931355
>10	0.7566589147625602	8.1459199354032
>50	0.06547275744150602	2.7256247980893527
>100	0.05447694366174264	6.33898088875775
>500	0.004761470915161627	1.868329061389119
>1k	8.402595732638165E-4	0.5555272982657622
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1373	0.11272577996715928	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	1.6420361247947455E-4	0.0
22	0.0	0.0	0.0	1.6420361247947455E-4	0.0
23	0.0	0.0	0.0	2.463054187192118E-4	0.0
24	0.0	0.0	0.0	4.1050903119868636E-4	0.0
25	0.0	0.0	0.0	6.568144499178982E-4	0.0
26	0.0	0.0	0.0	0.0010673234811165845	0.0
27	0.0	0.0	0.0	0.0013136288998357964	0.0
28	0.0	0.0	0.0	0.002134646962233169	0.0
29	0.0	0.0	0.0	0.003037766830870279	0.0
30	0.0	0.0	0.0	0.005747126436781609	0.0
31	0.0	0.0	0.0	0.011001642036124795	0.0
32	0.0	0.0	0.0	0.01683087027914614	0.0
33	0.0	0.0	0.0	0.02257799671592775	0.0
34	0.0	0.0	0.0	0.02889983579638752	0.0
35	0.0	0.0	0.0	0.03555008210180624	0.0
36	0.0	0.0	0.0	0.04384236453201971	0.0
37	0.0	0.0	0.0	0.05845648604269294	0.0
38	0.0	0.0	0.0	0.07931034482758621	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1635	0.0	24.897055	1
GGTATCA	800	0.0	24.203974	1
CGTTGCG	55	0.0044846972	19.999178	16
GTATTAT	125	2.2837412E-8	19.363178	1
TCGCGTG	60	0.00741389	18.332579	25
CTAGATA	265	0.0	18.263403	3
TACGCTC	85	1.430844E-4	18.117647	34
TAACGGC	75	0.0012908782	17.6	36
CTATTCC	240	0.0	17.41595	4
GTATTAC	115	4.2662577E-6	17.220219	1
TCGATTT	115	4.2746105E-6	17.216684	30
TAGGACG	115	4.2746105E-6	17.216684	4
ATCGATT	115	4.2746105E-6	17.216684	29
ATCAACG	2325	0.0	17.03156	3
CCTATTC	300	0.0	16.865974	3
GTCCTAA	235	0.0	16.853832	1
TCAACGC	2345	0.0	16.792488	4
CAACGCA	2410	0.0	16.522142	5
AACGCAG	2410	0.0	16.522142	6
GTATTAG	270	0.0	16.298973	1
>>END_MODULE