FastQCFastQC Report
Thu 2 Feb 2017
SRR4062077_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062077_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3074977
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57230.186115213219481No Hit
TATCAACGCAGAGTACTTTTTTTTT34540.11232604341430846No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATTG655.4543318E-513.1542425
GTATCAA30600.013.1274011
GGTATCA10100.012.9753051
CCGACCA2200.011.6607699
CGTCGTA2500.011.40201410
TTTAGAC1251.837725E-811.3984853
CGTCTTA855.322274E-511.17735215
CGTTGCA600.005888679511.0805982
TACGCTG1303.268724E-810.9618675
TAGGACA3450.010.7384034
CTTACAC4000.010.686083
TTTGCGC909.529209E-510.55466817
CCGTCGT2001.8189894E-1210.4515069
GTAGGAC4750.010.3986193
TAACGCC1856.002665E-1110.2695754
CGCAAGA4550.010.2282442
TCTTATA2053.6379788E-1210.1926062
AGTATCG750.002646739610.1342978
GTATTAT2350.010.1025721
CGACCAT2550.010.060610
Produced by FastQC (version 0.11.5)