##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062077_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3074977
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2817481236445	32.0	32.0	32.0	32.0	32.0
2	30.86099245620374	32.0	32.0	32.0	32.0	32.0
3	30.87657175972373	32.0	32.0	32.0	32.0	32.0
4	30.88386352158081	32.0	32.0	32.0	32.0	32.0
5	30.822697535623842	32.0	32.0	32.0	32.0	32.0
6	34.470363843371835	36.0	36.0	36.0	32.0	36.0
7	34.41711466459749	36.0	36.0	36.0	32.0	36.0
8	34.37780542748775	36.0	36.0	36.0	32.0	36.0
9	34.49422711129221	36.0	36.0	36.0	32.0	36.0
10	34.21741983761179	36.0	36.0	36.0	32.0	36.0
11	34.475531036492306	36.0	36.0	36.0	32.0	36.0
12	34.323569574666735	36.0	36.0	36.0	32.0	36.0
13	34.38581231664497	36.0	36.0	36.0	32.0	36.0
14	34.30858799919479	36.0	36.0	36.0	32.0	36.0
15	34.256375250936834	36.0	36.0	36.0	32.0	36.0
16	34.26495645333282	36.0	36.0	36.0	32.0	36.0
17	34.214314448530835	36.0	36.0	36.0	32.0	36.0
18	34.21068775473768	36.0	36.0	36.0	32.0	36.0
19	34.215068925718796	36.0	36.0	36.0	32.0	36.0
20	34.201327684727396	36.0	36.0	36.0	32.0	36.0
21	34.18614870940498	36.0	36.0	36.0	32.0	36.0
22	34.150633647015894	36.0	36.0	36.0	32.0	36.0
23	34.12799640452595	36.0	36.0	36.0	32.0	36.0
24	34.09478444879425	36.0	36.0	36.0	32.0	36.0
25	33.74976951047113	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	42.0
5	222.0
6	589.0
7	136.0
8	467.0
9	424.0
10	234.0
11	57.0
12	125.0
13	121.0
14	551.0
15	767.0
16	1188.0
17	1506.0
18	1999.0
19	2834.0
20	4214.0
21	5671.0
22	8460.0
23	12608.0
24	17630.0
25	24586.0
26	33109.0
27	43014.0
28	57011.0
29	75584.0
30	99009.0
31	135143.0
32	192657.0
33	276534.0
34	622166.0
35	1456316.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.812706954853844	16.525558497612643	11.393078122347514	26.268656425186006
2	16.963585649156137	18.93209329915196	36.992633096463734	27.111687955228163
3	19.528768007138954	22.238493431298803	27.78080762650323	30.451930935059014
4	13.356323326951077	14.958641103389597	34.22414951063057	37.46088605902875
5	15.508496823877183	35.6042278038185	33.0040913705815	15.88318400172282
6	35.74615607888157	33.83067093067601	16.140694085964824	14.2824789044776
7	31.389931613675703	29.728827894145898	19.57264325660449	19.30859723557391
8	28.526144790900272	32.90786329572165	18.581064803661768	19.984927109716317
9	27.158869960553066	14.643225975331667	17.98263892054223	40.21526514357304
10	16.389603995537648	26.580400165938027	30.846075546087853	26.18392029243647
11	36.99025556707272	21.201069826255814	21.86774105202621	19.940933554645255
12	24.838628481295622	23.058079419241626	28.258828747070595	23.844463352392154
13	29.492172555118046	19.471304361416667	24.81950754449184	26.217015538973442
14	23.612450456867933	18.975434680550574	24.77996565921097	32.63214920337053
15	25.309625700838108	26.825337695044492	21.82593359874385	26.039103005373555
16	25.96178828383279	25.667591539271488	23.022262710269604	25.34835746662612
17	24.336564837651505	25.754877780762374	24.72141502446013	25.187142357125996
18	24.91428932330318	25.160800098708485	25.279009373074135	24.645901204914196
19	25.748155265918715	24.780062517028124	24.86739625985027	24.604385957202883
20	25.942088281178542	24.455838742152856	24.67765974448434	24.924413232184257
21	26.120715638363457	24.1222311262197	24.539934211682667	25.21711902373418
22	26.03584890243946	24.010164231242122	24.634718870658332	25.319267995660084
23	24.92983095639495	24.279270189873007	24.901873995144147	25.889024858587895
24	24.929970347658244	24.560569220804158	24.946895943390395	25.5625644881472
25	25.22573778548808	24.31888086395844	24.8783837884109	25.57699756214258
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	214.0
1	214.0
2	539.0
3	864.0
4	864.0
5	864.0
6	1928.5
7	2993.0
8	2993.0
9	2993.0
10	3529.0
11	4065.0
12	4065.0
13	4065.0
14	5302.5
15	6540.0
16	6540.0
17	6540.0
18	11397.0
19	16254.0
20	16254.0
21	16254.0
22	25182.5
23	34111.0
24	34111.0
25	34111.0
26	50646.0
27	67181.0
28	67181.0
29	67181.0
30	87953.5
31	108726.0
32	108726.0
33	108726.0
34	137321.0
35	165916.0
36	165916.0
37	165916.0
38	202012.5
39	238109.0
40	238109.0
41	238109.0
42	276259.5
43	314410.0
44	314410.0
45	314410.0
46	355353.5
47	396297.0
48	396297.0
49	396297.0
50	415802.0
51	435307.0
52	435307.0
53	435307.0
54	417117.5
55	398928.0
56	398928.0
57	398928.0
58	365550.5
59	332173.0
60	332173.0
61	332173.0
62	289789.5
63	247406.0
64	247406.0
65	247406.0
66	201161.5
67	154917.0
68	154917.0
69	154917.0
70	117845.5
71	80774.0
72	80774.0
73	80774.0
74	59249.0
75	37724.0
76	37724.0
77	37724.0
78	28529.0
79	19334.0
80	19334.0
81	19334.0
82	13252.5
83	7171.0
84	7171.0
85	7171.0
86	5084.5
87	2998.0
88	2998.0
89	2998.0
90	2009.5
91	1021.0
92	1021.0
93	1021.0
94	663.0
95	305.0
96	305.0
97	305.0
98	772.0
99	1239.0
100	1239.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010081376218423748
2	2.6016454757222575E-4
3	5.203290951444515E-4
4	0.009951293944637634
5	0.030764457750415693
6	0.05895979059355566
7	0.10289507856481529
8	0.10526908006141183
9	0.11411467467886752
10	0.128488765932233
11	0.13652134633852545
12	0.1287164099113587
13	0.12344807782302113
14	0.11092765897110775
15	0.1304400000390247
16	0.11587078537498004
17	0.11876511596672105
18	0.1075129992842223
19	0.09281370234639154
20	0.08715512343669563
21	0.08777301423717966
22	0.09847228125608744
23	0.07795180256632814
24	0.08803317878475188
25	0.08478112194009906
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3074977.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.1597822272782
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.25779482097826	34.076618679806856
2	14.468336775841303	13.646472230791925
3	5.128371582301501	7.255586610057025
4	2.3620964615415954	4.455838189045042
5	1.3288575580238353	3.1334316523738392
6	0.8637134958658363	2.4439524223076394
7	0.5793967860621174	1.9126958377712049
8	0.4294181800985093	1.6201014286303859
9	0.34355423689586595	1.4581748695741004
>10	2.02000319423161	17.753713329099842
>50	0.14882077809409683	4.774605092676941
>100	0.06477352657890448	5.395631952193839
>500	0.0036122197332005097	1.1051880899747024
>1k	0.0011809179893618987	0.7805046904687206
>5k	6.946576408011168E-5	0.1874849252278064
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5723	0.186115213219481	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3454	0.11232604341430846	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.252056844652822E-5	1.6260284223264108E-4
2	0.0	0.0	0.0	3.252056844652822E-5	1.6260284223264108E-4
3	0.0	0.0	0.0	3.252056844652822E-5	1.6260284223264108E-4
4	0.0	0.0	0.0	3.252056844652822E-5	1.6260284223264108E-4
5	0.0	0.0	0.0	3.252056844652822E-5	1.951234106791693E-4
6	0.0	0.0	0.0	3.252056844652822E-5	3.577262529118104E-4
7	0.0	0.0	0.0	3.252056844652822E-5	3.577262529118104E-4
8	0.0	0.0	0.0	3.252056844652822E-5	3.577262529118104E-4
9	0.0	0.0	0.0	3.252056844652822E-5	3.577262529118104E-4
10	0.0	0.0	0.0	3.252056844652822E-5	3.577262529118104E-4
11	0.0	0.0	0.0	3.252056844652822E-5	3.577262529118104E-4
12	0.0	0.0	0.0	3.252056844652822E-5	7.47973074270149E-4
13	0.0	0.0	0.0	3.252056844652822E-5	9.756170533958466E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGATTG	65	5.4543318E-5	13.154242	5
GTATCAA	3060	0.0	13.127401	1
GGTATCA	1010	0.0	12.975305	1
CCGACCA	220	0.0	11.660769	9
CGTCGTA	250	0.0	11.402014	10
TTTAGAC	125	1.837725E-8	11.398485	3
CGTCTTA	85	5.322274E-5	11.177352	15
CGTTGCA	60	0.0058886795	11.080598	2
TACGCTG	130	3.268724E-8	10.961867	5
TAGGACA	345	0.0	10.738403	4
CTTACAC	400	0.0	10.68608	3
TTTGCGC	90	9.529209E-5	10.554668	17
CCGTCGT	200	1.8189894E-12	10.451506	9
GTAGGAC	475	0.0	10.398619	3
TAACGCC	185	6.002665E-11	10.269575	4
CGCAAGA	455	0.0	10.228244	2
TCTTATA	205	3.6379788E-12	10.192606	2
AGTATCG	75	0.0026467396	10.134297	8
GTATTAT	235	0.0	10.102572	1
CGACCAT	255	0.0	10.0606	10
>>END_MODULE