FastQCFastQC Report
Thu 2 Feb 2017
SRR4062077_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062077_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3074977
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34550.027.9631561
GGTATCA14000.025.4657631
TCAACGC48550.019.6653044
CAACGCA50100.019.1008075
ATCAACG50750.018.8128193
TATCAAC51350.018.7227422
AACGCAG51350.018.5930026
TATACCG2450.017.0603435
CGTCTTA2301.8189894E-1216.26002915
GTATAGG4850.015.4279021
TTAACGG1158.226606E-515.30380535
AACGTCG1301.3701392E-515.23097122
GTATTAG3050.015.1526711
ACGCAGA63250.015.060097
TAGGACG3550.014.872474
CGCAATA3850.014.857136
GTCCTAC6400.014.7862561
ACGTCGT1351.9576713E-514.66686223
CGCGTAA3150.014.66590810
GTAATAC3500.014.4563943
Produced by FastQC (version 0.11.5)