FastQCFastQC Report
Thu 2 Feb 2017
SRR4062076_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062076_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences958883
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19360.20190158757637794No Hit
GCGCAAGACGGACCAGAGCGAAAGC15850.16529649602714827No Hit
TATCAACGCAGAGTACTTTTTTTTT12840.13390580498350685No Hit
GAATAGGACCGCGGTTCTATTTTGT12570.13109002871048916No Hit
GGGTAGGCACACGCTGAGCCAGTCA12430.12962999656892446No Hit
GATTAAGAGGGACGGCCGGGGGCAT12170.12691850830601856No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG11640.12139124377009498No Hit
GAATAACGCCGCCGCATCGCCAGTC11610.1210783797397597No Hit
GTCTTGCGCCGGTCCAAGAATTTCA11560.12055693968920086No Hit
ATCAGATACCGTCGTAGTTCCGACC11300.11784545142629498No Hit
GAACTACGACGGTATCTGATCGTCT10990.11461252311283025No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC10910.1137782190319361No Hit
TCGTAGTTCCGACCATAAACGATGC10790.11252676291059494No Hit
GTATCTGATCGTCTTCGAACCTCCG10230.10668663434433606No Hit
ACCATACTCCCCCCGGAACCCAAAG9960.1038708580713184No Hit
GTGCATGGCCGTTCTTAGTTGGTGG9650.10063792975785367No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGCC250.006036840418.9965023
GTAATAC402.7636078E-416.6219393
GGTTTAT402.766912E-416.6193351
AAGACGG3450.014.5938025
CCGACGC400.005276278614.25035319
CAAGACG3750.013.6796254
TGTACCG500.001499124313.2996355
GAATTTC1301.4551915E-1113.15485918
CGCAATA655.4603206E-513.1507382
AACCGCG604.08682E-412.6676437
AGAATTT1501.8189894E-1212.66764217
AGCCCCG2250.012.66764116
CGGTCCA1301.9645086E-1012.42338410
CGCAAGA3450.012.3883762
CGGACCA3700.012.3252739
GCGCAAG3600.012.1347531
CGAACGA802.8614133E-511.87591416
AATACTG802.8644741E-511.8746745
ATTACAC802.8690707E-511.8728133
TGCGGTA1459.640644E-1111.79339515
Produced by FastQC (version 0.11.5)