##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062076_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	958883
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.247457719033502	32.0	32.0	32.0	32.0	32.0
2	30.781588577542827	32.0	32.0	32.0	32.0	32.0
3	30.817946506508093	32.0	32.0	32.0	32.0	32.0
4	30.817311392526513	32.0	32.0	32.0	32.0	32.0
5	30.73774485521174	32.0	32.0	32.0	32.0	32.0
6	34.38970239330554	36.0	36.0	36.0	32.0	36.0
7	34.317276456043125	36.0	36.0	36.0	32.0	36.0
8	34.25882824077598	36.0	36.0	36.0	32.0	36.0
9	34.39468318866848	36.0	36.0	36.0	32.0	36.0
10	34.084373171700825	36.0	36.0	36.0	32.0	36.0
11	34.39291446401699	36.0	36.0	36.0	32.0	36.0
12	34.196180347341645	36.0	36.0	36.0	32.0	36.0
13	34.294240277489536	36.0	36.0	36.0	32.0	36.0
14	34.19541278758722	36.0	36.0	36.0	32.0	36.0
15	34.146685257742604	36.0	36.0	36.0	32.0	36.0
16	34.145036464302734	36.0	36.0	36.0	32.0	36.0
17	34.07351887560839	36.0	36.0	36.0	32.0	36.0
18	34.089008773750294	36.0	36.0	36.0	32.0	36.0
19	34.082847438112886	36.0	36.0	36.0	32.0	36.0
20	34.05215443385689	36.0	36.0	36.0	32.0	36.0
21	34.03142927760738	36.0	36.0	36.0	32.0	36.0
22	34.00021587618093	36.0	36.0	36.0	32.0	36.0
23	33.96409676675882	36.0	36.0	36.0	32.0	36.0
24	33.93881005294702	36.0	36.0	36.0	32.0	36.0
25	33.58975912598304	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	25.0
5	95.0
6	210.0
7	50.0
8	195.0
9	128.0
10	69.0
11	20.0
12	49.0
13	58.0
14	195.0
15	231.0
16	312.0
17	452.0
18	654.0
19	894.0
20	1341.0
21	1939.0
22	2827.0
23	4250.0
24	5935.0
25	8454.0
26	11356.0
27	14407.0
28	19408.0
29	25618.0
30	32994.0
31	45224.0
32	64326.0
33	90723.0
34	198403.0
35	428041.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.795797988056826	17.558928493211827	11.033983609942496	25.611289908788848
2	16.851812840370346	19.35670916755138	37.79849866824072	25.992979323837552
3	19.779408390657395	22.753202708170818	27.841660444103304	29.625728457068483
4	12.666581140151884	15.370992118001558	35.2585143209977	36.70391242084886
5	14.722003718162451	36.6788797105592	33.24229190705012	15.356824664228231
6	35.087785211925386	35.14257595823797	16.18236865092659	13.58727017891005
7	30.943471478896033	29.90471735739448	19.65760475765884	19.494206406050647
8	27.946394077662806	34.136447641819714	18.565357668654006	19.351800611863467
9	26.682378542742637	14.278628576679456	18.650108284551333	40.38888459602658
10	16.937301436446088	26.624905222130085	30.292887116684874	26.144906224738957
11	37.97658338903743	20.90898646390374	21.575033422459892	19.53939672459893
12	24.552264058720564	23.693555192567185	28.601522323088428	23.152658425623823
13	29.929998506606957	19.382779611738695	24.81173326538221	25.875488616272136
14	23.098032663631425	20.332275176475502	24.559333361179565	32.010358798713504
15	25.358495374286928	27.887545771477868	21.48935636894579	25.264602485289416
16	25.45172969422419	26.02869474319573	24.093433876672545	24.426141685907538
17	23.55703106969089	26.555599301138212	25.155787070639136	24.731582558531766
18	24.550219799726424	25.07909657613633	26.653510008457854	23.717173615679396
19	25.813796883564326	24.813927387570796	24.782293216401744	24.58998251246313
20	25.765726640365553	24.636045604829828	24.688139758487747	24.91008799631687
21	26.73405516907325	24.308403136770195	24.125612780918818	24.83192891323774
22	25.987133335560603	24.84871959775491	24.82961379126019	24.334533275424295
23	24.264240002588664	24.846793866118038	25.591034870770322	25.297931260522972
24	24.955136001336264	25.112146947212377	25.479306187003797	24.453410864447562
25	24.720149740636774	24.943340957467353	25.34692245618907	24.989586845706803
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1018.0
1	1018.0
2	701.0
3	384.0
4	384.0
5	384.0
6	826.5
7	1269.0
8	1269.0
9	1269.0
10	1478.5
11	1688.0
12	1688.0
13	1688.0
14	2065.0
15	2442.0
16	2442.0
17	2442.0
18	4051.0
19	5660.0
20	5660.0
21	5660.0
22	9460.5
23	13261.0
24	13261.0
25	13261.0
26	20618.5
27	27976.0
28	27976.0
29	27976.0
30	36393.5
31	44811.0
32	44811.0
33	44811.0
34	52981.5
35	61152.0
36	61152.0
37	61152.0
38	68638.0
39	76124.0
40	76124.0
41	76124.0
42	87006.0
43	97888.0
44	97888.0
45	97888.0
46	109032.5
47	120177.0
48	120177.0
49	120177.0
50	122943.5
51	125710.0
52	125710.0
53	125710.0
54	117143.0
55	108576.0
56	108576.0
57	108576.0
58	101328.0
59	94080.0
60	94080.0
61	94080.0
62	84216.0
63	74352.0
64	74352.0
65	74352.0
66	61348.0
67	48344.0
68	48344.0
69	48344.0
70	36845.0
71	25346.0
72	25346.0
73	25346.0
74	19484.0
75	13622.0
76	13622.0
77	13622.0
78	11287.0
79	8952.0
80	8952.0
81	8952.0
82	6129.5
83	3307.0
84	3307.0
85	3307.0
86	2502.5
87	1698.0
88	1698.0
89	1698.0
90	1139.0
91	580.0
92	580.0
93	580.0
94	338.5
95	97.0
96	97.0
97	97.0
98	233.0
99	369.0
100	369.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.38592091005889E-4
2	1.0428801011176547E-4
3	7.300160707823582E-4
4	0.012097409172964793
5	0.03597936348855908
6	0.0721673029973417
7	0.11482109913305379
8	0.12024407565886558
9	0.12733566034646562
10	0.14402174196434808
11	0.15048759859127755
12	0.14589892614635988
13	0.1389116294688716
14	0.12962999656892446
15	0.14694180624747752
16	0.13411438100373038
17	0.13828590140820102
18	0.1244155960633362
19	0.11033671469824785
20	0.10449658613198899
21	0.09959504965673602
22	0.11044100270835963
23	0.0896876886961183
24	0.10303655399042427
25	0.10011648970729484
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	958883.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.63056892187853
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.36674353808833	36.41233221740879
2	15.402844313976589	15.289038526355595
3	5.204344860841862	7.748837889277091
4	2.2127409243805047	4.392783638149113
5	1.1008505289555803	2.731791902500818
6	0.6323937319101942	1.8831636418399715
7	0.4062138492806058	1.4112437110619875
8	0.2518256760097643	0.9998601255601006
9	0.19232045462816297	0.859047622065905
>10	1.0144306726265913	9.423406969350253
>50	0.10217171953092208	3.5004095605563736
>100	0.09942739755595251	9.95615549574854
>500	0.010743214507089082	3.570189886000429
>1k	0.002949117707828375	1.8217388141250839
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1936	0.20190158757637794	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1585	0.16529649602714827	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1284	0.13390580498350685	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1257	0.13109002871048916	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1243	0.12962999656892446	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1217	0.12691850830601856	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1164	0.12139124377009498	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1161	0.1210783797397597	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1156	0.12055693968920086	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1130	0.11784545142629498	No Hit
GAACTACGACGGTATCTGATCGTCT	1099	0.11461252311283025	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1091	0.1137782190319361	No Hit
TCGTAGTTCCGACCATAAACGATGC	1079	0.11252676291059494	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1023	0.10668663434433606	No Hit
ACCATACTCCCCCCGGAACCCAAAG	996	0.1038708580713184	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	965	0.10063792975785367	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.1715204044706183E-4
2	0.0	0.0	0.0	0.0	4.1715204044706183E-4
3	0.0	0.0	0.0	0.0	4.1715204044706183E-4
4	0.0	0.0	0.0	0.0	4.1715204044706183E-4
5	0.0	0.0	0.0	0.0	4.1715204044706183E-4
6	0.0	0.0	0.0	0.0	5.214400505588273E-4
7	0.0	0.0	0.0	1.0428801011176546E-4	6.257280606705928E-4
8	0.0	0.0	0.0	1.0428801011176546E-4	6.257280606705928E-4
9	0.0	0.0	0.0	1.0428801011176546E-4	6.257280606705928E-4
10	0.0	0.0	0.0	1.0428801011176546E-4	6.257280606705928E-4
11	0.0	0.0	0.0	1.0428801011176546E-4	6.257280606705928E-4
12	0.0	0.0	0.0	1.0428801011176546E-4	7.300160707823583E-4
13	0.0	0.0	0.0	1.0428801011176546E-4	7.300160707823583E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTCGCC	25	0.0060368404	18.996502	3
GTAATAC	40	2.7636078E-4	16.621939	3
GGTTTAT	40	2.766912E-4	16.619335	1
AAGACGG	345	0.0	14.593802	5
CCGACGC	40	0.0052762786	14.250353	19
CAAGACG	375	0.0	13.679625	4
TGTACCG	50	0.0014991243	13.299635	5
GAATTTC	130	1.4551915E-11	13.154859	18
CGCAATA	65	5.4603206E-5	13.150738	2
AACCGCG	60	4.08682E-4	12.667643	7
AGAATTT	150	1.8189894E-12	12.667642	17
AGCCCCG	225	0.0	12.667641	16
CGGTCCA	130	1.9645086E-10	12.423384	10
CGCAAGA	345	0.0	12.388376	2
CGGACCA	370	0.0	12.325273	9
GCGCAAG	360	0.0	12.134753	1
CGAACGA	80	2.8614133E-5	11.875914	16
AATACTG	80	2.8644741E-5	11.874674	5
ATTACAC	80	2.8690707E-5	11.872813	3
TGCGGTA	145	9.640644E-11	11.793395	15
>>END_MODULE