FastQCFastQC Report
Thu 2 Feb 2017
SRR4062076_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062076_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences958883
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16220.1691551524012836No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA12730.13275863687227743No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12550.13088145269026563No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12260.12785710039702444No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC12240.1276485243768009No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA11520.1201397876487538No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11460.11951405958808323No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG11440.1193054835678597No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC11340.11826260346674204No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10890.11356964301171257No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT9940.10366228205109487No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG9690.10105508179830074No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC9610.10022077771740662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATAC1006.002665E-1126.3985613
CAGTACG450.00139844724.4431139
GTATCAA12750.024.3407761
GATTGCG551.5938724E-423.99869310
GGTATCA5900.023.8755571
GATCGAA651.8136487E-523.69101511
GTGATCG752.0645057E-623.465399
TAGGACG4750.022.69354
TGTAGGA7100.021.072572
GTAGGAC7150.020.9219363
CGCAATA2850.020.84314236
TACACTA654.9329526E-420.3065855
ACCGCGC550.00448436219.998918
GGACGTG5250.019.6941646
GTCCTAG1705.456968E-1219.4208341
GTCCTAC4000.019.2589931
CGTCTTA808.989864E-519.24895115
AGGACGT5150.019.2222525
CTGTAGG7450.018.908161
AATACGA3150.018.85808239
Produced by FastQC (version 0.11.5)