##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062076_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	958883
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.227438592612447	32.0	32.0	32.0	32.0	32.0
2	31.438530039639872	32.0	32.0	32.0	32.0	32.0
3	31.574492404182784	32.0	32.0	32.0	32.0	32.0
4	31.65518420912666	32.0	32.0	32.0	32.0	32.0
5	31.620918297644238	32.0	32.0	32.0	32.0	32.0
6	35.27667191930611	36.0	36.0	36.0	36.0	36.0
7	35.27410747713746	36.0	36.0	36.0	36.0	36.0
8	35.226483314439825	36.0	36.0	36.0	36.0	36.0
9	35.31217572946856	36.0	36.0	36.0	36.0	36.0
10	35.19148321536621	36.0	36.0	36.0	36.0	36.0
11	35.31168661870113	36.0	36.0	36.0	36.0	36.0
12	35.23093432671139	36.0	36.0	36.0	36.0	36.0
13	35.26793362693884	36.0	36.0	36.0	36.0	36.0
14	35.22738853436759	36.0	36.0	36.0	36.0	36.0
15	35.204288740127836	36.0	36.0	36.0	36.0	36.0
16	35.215715577395784	36.0	36.0	36.0	36.0	36.0
17	35.19231751944711	36.0	36.0	36.0	36.0	36.0
18	35.19355020372663	36.0	36.0	36.0	36.0	36.0
19	35.186299058383554	36.0	36.0	36.0	36.0	36.0
20	35.17198135747531	36.0	36.0	36.0	36.0	36.0
21	35.159647214519396	36.0	36.0	36.0	36.0	36.0
22	35.14115277880617	36.0	36.0	36.0	36.0	36.0
23	35.11405458225873	36.0	36.0	36.0	36.0	36.0
24	35.093695476924715	36.0	36.0	36.0	36.0	36.0
25	35.066905972887206	36.0	36.0	36.0	36.0	36.0
26	35.029883729297524	36.0	36.0	36.0	36.0	36.0
27	35.0228578460563	36.0	36.0	36.0	36.0	36.0
28	34.98732587813112	36.0	36.0	36.0	36.0	36.0
29	34.95886672305172	36.0	36.0	36.0	36.0	36.0
30	34.93607874996219	36.0	36.0	36.0	36.0	36.0
31	34.9454146126274	36.0	36.0	36.0	36.0	36.0
32	34.911745228562815	36.0	36.0	36.0	32.0	36.0
33	34.8923153293989	36.0	36.0	36.0	32.0	36.0
34	34.881424532502926	36.0	36.0	36.0	32.0	36.0
35	34.857921143664036	36.0	36.0	36.0	32.0	36.0
36	34.83118899803208	36.0	36.0	36.0	32.0	36.0
37	34.8143141551159	36.0	36.0	36.0	32.0	36.0
38	34.78582997091407	36.0	36.0	36.0	32.0	36.0
39	34.75278527203006	36.0	36.0	36.0	32.0	36.0
40	34.739045326697834	36.0	36.0	36.0	32.0	36.0
41	34.719174289251136	36.0	36.0	36.0	32.0	36.0
42	34.67127897772721	36.0	36.0	36.0	32.0	36.0
43	34.679599075173925	36.0	36.0	36.0	32.0	36.0
44	34.6066965417053	36.0	36.0	36.0	32.0	36.0
45	34.59669740729578	36.0	36.0	36.0	32.0	36.0
46	34.553815220417924	36.0	36.0	36.0	32.0	36.0
47	34.528801741192616	36.0	36.0	36.0	32.0	36.0
48	34.49431682488896	36.0	36.0	36.0	32.0	36.0
49	34.46531850079728	36.0	36.0	36.0	32.0	36.0
50	34.064959958618516	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	8.0
21	19.0
22	67.0
23	192.0
24	481.0
25	1143.0
26	2417.0
27	4662.0
28	7912.0
29	12607.0
30	18595.0
31	27654.0
32	41601.0
33	71034.0
34	160637.0
35	609851.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.36512903966118	18.07546264421773	11.451670109115186	26.107738207005905
2	16.224765317583895	19.670980519351847	37.68893746564892	26.415316697415335
3	19.286459202402803	23.021128816953112	28.225221091273152	29.467190889370933
4	12.363760750790242	15.598670536447095	35.44186308444304	36.59570562831962
5	14.390181075271958	36.91847701961553	33.160145711207726	15.531196193904783
6	35.24399795177921	35.26183123870428	16.011371588340996	13.482799221175515
7	30.85183489539391	29.989685915799946	19.75934498786609	19.39913420094005
8	28.173614507713662	33.96045190080542	18.54960407056961	19.31632952091131
9	26.86730289305369	13.881672737966987	18.692582932432842	40.55844143654648
10	16.853585738391168	26.590028804378434	30.49989466899994	26.05649078823046
11	38.14688549072201	20.800660768832067	21.543087112817727	19.50936662762819
12	24.59601432082955	23.504744583020035	28.666375355491752	23.232865740658664
13	30.01242070200431	19.181902275877246	24.921288624368145	25.884388397750296
14	23.201787913645354	19.99096865832432	24.667138743725772	32.140104684304546
15	25.284940915627875	27.883276687562507	21.585115180892767	25.24666721591685
16	25.598983808091575	25.791084125755837	24.151139774360345	24.458792291792246
17	23.83711047124623	26.317496503744458	25.17376989684873	24.671623128160576
18	24.64930549399666	24.94871637102754	26.75164748983974	23.65033064513606
19	25.940703923210652	24.574113838706076	24.869040331302152	24.61614190678112
20	25.890985543580964	24.430639015019576	24.95385250739924	24.72452293400022
21	26.880443182327774	24.040472090964172	24.304946484607612	24.774138242100445
22	25.98930213592274	24.626257843761962	25.050084316856175	24.334355703459128
23	24.33677970803498	24.573826602230515	25.86971076733112	25.21968292240338
24	25.11138040789424	24.79705405078446	25.608107826246563	24.48345771507473
25	24.87983388971103	24.63298365490608	25.526108036346535	24.961074419036354
26	24.048990693270035	25.967382643845504	26.097117875512577	23.88650878737188
27	24.804198240026366	24.853943880243367	25.605968642517606	24.735889237212657
28	24.32222161965985	25.02701101715773	25.833795684077476	24.81697167910494
29	24.307004004672116	24.792466210579008	25.605185216085435	25.29534456866344
30	24.108589866864676	25.33169112938718	25.986730269044735	24.57298873470341
31	25.228131211686993	24.83141755259793	24.952913717907805	24.987537517807272
32	24.822266046635804	24.842289469869442	25.04460947545931	25.29083500803544
33	24.228787752377773	24.683902052394462	25.817307692307693	25.270002502920075
34	25.120766397323536	24.92334775299413	25.895319102782842	24.060566746899497
35	25.409540297013184	24.78068162856666	25.92190889370933	23.88786918071083
36	23.797764058067745	25.571917236776237	25.58891623560821	25.04140246954781
37	25.106063007892672	25.156122244656174	25.178857481352935	24.558957266098215
38	24.42842348857994	24.990535863082357	25.54878958121064	25.032251067127063
39	25.66082993523627	24.134449925433064	25.148351705653532	25.05636843367714
40	25.98786920601284	24.655797063663726	25.426069109650612	23.930264620672826
41	24.33466301655586	25.17669143527571	26.476756615825835	24.01188893234259
42	25.7200457224862	25.72682483198238	25.28524406880066	23.26788537673076
43	24.70936083893475	24.74179603372843	25.74332392956035	24.80551919777647
44	24.095869109734693	25.469182321721146	25.316916427925566	25.118032140618595
45	24.382864758996565	25.626417038460016	25.281113315103465	24.709604887439955
46	24.297287601057928	25.10867116647338	25.208477602476286	25.385563629992408
47	24.92796245600313	24.857046017468388	25.706270368921913	24.50872115760657
48	25.44663250045367	25.37769517814197	24.435099839181014	24.74057248222335
49	24.657919986650537	25.76279671269451	24.693066622168452	24.886216678486505
50	24.723162569118102	25.930004818191488	24.635454797646386	24.71137781504402
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	584.0
1	319.0
2	54.0
3	168.5
4	283.0
5	270.5
6	258.0
7	197.0
8	136.0
9	199.5
10	263.0
11	413.5
12	564.0
13	1048.0
14	1532.0
15	1948.5
16	2365.0
17	2347.5
18	2330.0
19	2289.5
20	2249.0
21	2609.5
22	2970.0
23	3296.0
24	3622.0
25	4814.5
26	6007.0
27	8252.5
28	10498.0
29	12154.5
30	13811.0
31	15479.0
32	17147.0
33	20107.0
34	23067.0
35	27249.5
36	31432.0
37	35594.5
38	39757.0
39	41496.5
40	43236.0
41	45396.0
42	47556.0
43	48689.0
44	49822.0
45	53829.0
46	57836.0
47	61787.5
48	65739.0
49	68758.0
50	71777.0
51	68868.0
52	65959.0
53	65098.5
54	64238.0
55	66458.5
56	68679.0
57	66665.5
58	64652.0
59	59963.0
60	55274.0
61	48624.5
62	41975.0
63	36470.0
64	30965.0
65	26243.5
66	21522.0
67	18673.0
68	15824.0
69	14503.0
70	13182.0
71	10288.5
72	7395.0
73	6730.5
74	6066.0
75	4325.5
76	2585.0
77	2347.5
78	2110.0
79	1788.0
80	1466.0
81	1201.0
82	936.0
83	768.0
84	600.0
85	487.0
86	374.0
87	240.0
88	106.0
89	73.5
90	41.0
91	29.5
92	18.0
93	12.0
94	6.0
95	6.5
96	7.0
97	6.0
98	5.0
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02742774665939432
2	0.0035457923438000256
3	3.128640303352964E-4
4	0.0
5	0.0
6	2.0857602022353094E-4
7	0.0
8	0.0
9	0.0
10	1.0428801011176547E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	9.38592091005889E-4
17	0.0
18	0.0
19	0.0
20	1.0428801011176547E-4
21	0.0
22	0.0
23	1.0428801011176547E-4
24	7.300160707823582E-4
25	2.0857602022353094E-4
26	7.300160707823582E-4
27	5.214400505588273E-4
28	0.0015643201516764818
29	3.128640303352964E-4
30	9.38592091005889E-4
31	5.214400505588273E-4
32	6.257280606705928E-4
33	3.128640303352964E-4
34	7.300160707823582E-4
35	3.128640303352964E-4
36	3.128640303352964E-4
37	0.001981472192123544
38	0.0
39	0.001355744131452951
40	1.0428801011176547E-4
41	8.343040808941238E-4
42	0.0057358405561471005
43	0.005005824485364742
44	0.0035457923438000256
45	0.0017728961719000128
46	0.0028157762730176674
47	8.343040808941238E-4
48	0.004275808414582384
49	0.0044843844348059145
50	0.0017728961719000128
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	958883.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.9064691248918
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.21779597200812	38.00477266013666
2	15.694972143944428	16.29341174011377
3	5.274847920096824	8.213961921090165
4	2.206849216342711	4.581990028455383
5	1.0801616564121141	2.8033688834223693
6	0.6081447727162116	1.8939988721075027
7	0.3800740898435167	1.3809812806743695
8	0.2643011451842871	1.0975151385745394
9	0.18015198835346152	0.8415948259131134
>10	0.9096160949137949	8.711351275888992
>50	0.09144534868831684	3.33975095957208
>100	0.08114348006190217	8.652986196736446
>500	0.008477676927773133	2.8992763193411997
>1k	0.0020184945066126503	1.285039897973396
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1622	0.1691551524012836	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1273	0.13275863687227743	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1255	0.13088145269026563	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1226	0.12785710039702444	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1224	0.1276485243768009	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1152	0.1201397876487538	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	1146	0.11951405958808323	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1144	0.1193054835678597	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1134	0.11826260346674204	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1089	0.11356964301171257	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT	994	0.10366228205109487	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	969	0.10105508179830074	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	961	0.10022077771740662	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	1.0428801011176546E-4
4	0.0	0.0	0.0	0.0	1.0428801011176546E-4
5	0.0	0.0	0.0	0.0	1.0428801011176546E-4
6	0.0	0.0	0.0	0.0	2.0857602022353092E-4
7	0.0	0.0	0.0	0.0	2.0857602022353092E-4
8	0.0	0.0	0.0	0.0	2.0857602022353092E-4
9	0.0	0.0	0.0	0.0	2.0857602022353092E-4
10	0.0	0.0	0.0	1.0428801011176546E-4	2.0857602022353092E-4
11	0.0	0.0	0.0	1.0428801011176546E-4	2.0857602022353092E-4
12	0.0	0.0	0.0	1.0428801011176546E-4	3.128640303352964E-4
13	0.0	0.0	0.0	1.0428801011176546E-4	3.128640303352964E-4
14	0.0	0.0	0.0	1.0428801011176546E-4	3.128640303352964E-4
15	0.0	0.0	0.0	1.0428801011176546E-4	3.128640303352964E-4
16	0.0	0.0	0.0	1.0428801011176546E-4	3.128640303352964E-4
17	0.0	0.0	0.0	1.0428801011176546E-4	3.128640303352964E-4
18	0.0	0.0	0.0	3.128640303352964E-4	3.128640303352964E-4
19	0.0	0.0	0.0	3.128640303352964E-4	3.128640303352964E-4
20	0.0	0.0	0.0	4.1715204044706183E-4	3.128640303352964E-4
21	0.0	0.0	0.0	5.214400505588273E-4	3.128640303352964E-4
22	0.0	0.0	0.0	7.300160707823583E-4	3.128640303352964E-4
23	0.0	0.0	0.0	9.385920910058892E-4	3.128640303352964E-4
24	0.0	0.0	0.0	0.001355744131452951	3.128640303352964E-4
25	0.0	0.0	0.0	0.00229433622245884	3.128640303352964E-4
26	0.0	0.0	0.0	0.0028157762730176674	3.128640303352964E-4
27	0.0	0.0	0.0	0.003650080353911791	3.128640303352964E-4
28	0.0	0.0	0.0	0.0057358405561471005	3.128640303352964E-4
29	0.0	0.0	0.0	0.008551616829164768	3.128640303352964E-4
30	0.0	0.0	0.0	0.013974593354976571	3.128640303352964E-4
31	0.0	0.0	0.0	0.023777666305482524	3.128640303352964E-4
32	0.0	0.0	0.0	0.033372163235764946	3.128640303352964E-4
33	0.0	0.0	0.0	0.041923780064929715	3.128640303352964E-4
34	0.0	0.0	0.0	0.05245686908621803	4.1715204044706183E-4
35	0.0	0.0	0.0	0.065805734380524	4.1715204044706183E-4
36	0.0	0.0	0.0	0.08322183206918883	4.1715204044706183E-4
37	0.0	0.0	0.0	0.10950241061735373	4.1715204044706183E-4
38	0.0	0.0	0.0	0.14548177410591281	4.1715204044706183E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTATAC	100	6.002665E-11	26.398561	3
CAGTACG	45	0.001398447	24.443113	9
GTATCAA	1275	0.0	24.340776	1
GATTGCG	55	1.5938724E-4	23.998693	10
GGTATCA	590	0.0	23.875557	1
GATCGAA	65	1.8136487E-5	23.691015	11
GTGATCG	75	2.0645057E-6	23.46539	9
TAGGACG	475	0.0	22.6935	4
TGTAGGA	710	0.0	21.07257	2
GTAGGAC	715	0.0	20.921936	3
CGCAATA	285	0.0	20.843142	36
TACACTA	65	4.9329526E-4	20.306585	5
ACCGCGC	55	0.004484362	19.99891	8
GGACGTG	525	0.0	19.694164	6
GTCCTAG	170	5.456968E-12	19.420834	1
GTCCTAC	400	0.0	19.258993	1
CGTCTTA	80	8.989864E-5	19.248951	15
AGGACGT	515	0.0	19.222252	5
CTGTAGG	745	0.0	18.90816	1
AATACGA	315	0.0	18.858082	39
>>END_MODULE