FastQCFastQC Report
Thu 2 Feb 2017
SRR4062075_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062075_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031537
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36930.1817835461524944No Hit
TATCAACGCAGAGTACTTTTTTTTT22740.11193495368285195No Hit
GCGCAAGACGGACCAGAGCGAAAGC22630.11139349172572294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9000.015.1950911
CGTCTTA653.3760298E-614.61426615
AGCGCTA851.7193088E-814.52829914
CGATAAC1700.013.41470610
GTCTTAA1001.0124495E-813.2957051
CCGATAA1750.013.0311079
GTATCAA26400.012.9863351
TAACGAA1800.012.66632213
CGAACGA1800.012.66569716
CGTTATT1800.012.1361652
AGTAGCG550.003074164512.0881962
ACGAACG1900.011.99908215
CGTCCCG658.0171507E-411.69371911
CGGACCA4000.011.6397669
GCGTTAT1800.011.6073621
ATTCCGA1900.011.5019556
CGTCGTA3500.011.402510
GATAACG2000.011.40137611
GTACCGG752.0806435E-411.39631751
ATAACGA2050.011.12247112
Produced by FastQC (version 0.11.5)