FastQCFastQC Report
Thu 2 Feb 2017
SRR4062075_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062075_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031537
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT22100.10878462956864679No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT20980.10327156236878776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28850.029.5196531
GGTATCA10250.028.1250671
TCAACGC43850.019.3146844
GGCGTTA6600.019.00212742
TAGATCG708.129224E-418.8560055
ATCAACG45450.018.683143
CAACGCA45350.018.6758295
AACGCAG45750.018.5606276
TATCAAC45700.018.5341662
CAACGCG1456.31735E-918.20624521
AGCGCTA1701.2732926E-1018.1178914
ATTACTC1002.485652E-517.5989363
TCGATCG1903.274181E-1117.36950941
CGTTATT7250.017.29763444
AATCGGT800.00198912716.49981519
ATACCGT6700.016.416926
TACCGTC6600.016.3323467
CCTATTC5150.016.232033
GGACCGT1501.8406536E-716.1323596
CCGCCTA1653.1113814E-816.00100341
Produced by FastQC (version 0.11.5)