##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062075_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2031537
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.975529365204768	32.0	32.0	32.0	32.0	32.0
2	31.367610336410316	32.0	32.0	32.0	32.0	32.0
3	31.51062471419423	32.0	32.0	32.0	32.0	32.0
4	31.61347836637974	32.0	32.0	32.0	32.0	32.0
5	31.541548098804007	32.0	32.0	32.0	32.0	32.0
6	35.21352896846083	36.0	36.0	36.0	36.0	36.0
7	35.2251738462061	36.0	36.0	36.0	36.0	36.0
8	35.15645149460728	36.0	36.0	36.0	36.0	36.0
9	35.28043693026511	36.0	36.0	36.0	36.0	36.0
10	35.12966930949325	36.0	36.0	36.0	36.0	36.0
11	35.280641701332534	36.0	36.0	36.0	36.0	36.0
12	35.18792618593705	36.0	36.0	36.0	36.0	36.0
13	35.237057459450654	36.0	36.0	36.0	36.0	36.0
14	35.19739044870953	36.0	36.0	36.0	36.0	36.0
15	35.15830329450067	36.0	36.0	36.0	36.0	36.0
16	35.174416710106684	36.0	36.0	36.0	36.0	36.0
17	35.15830723240581	36.0	36.0	36.0	36.0	36.0
18	35.157164255438126	36.0	36.0	36.0	36.0	36.0
19	35.150977806458855	36.0	36.0	36.0	36.0	36.0
20	35.15393960336435	36.0	36.0	36.0	36.0	36.0
21	35.14081161209469	36.0	36.0	36.0	36.0	36.0
22	35.127214025636746	36.0	36.0	36.0	36.0	36.0
23	35.09216814658064	36.0	36.0	36.0	36.0	36.0
24	35.07673401961175	36.0	36.0	36.0	36.0	36.0
25	35.06232079455112	36.0	36.0	36.0	36.0	36.0
26	35.01284298538496	36.0	36.0	36.0	36.0	36.0
27	35.005846804660706	36.0	36.0	36.0	36.0	36.0
28	34.98477212081296	36.0	36.0	36.0	36.0	36.0
29	34.965350372648885	36.0	36.0	36.0	36.0	36.0
30	34.9389811753367	36.0	36.0	36.0	36.0	36.0
31	34.948785574665884	36.0	36.0	36.0	36.0	36.0
32	34.91940240320506	36.0	36.0	36.0	32.0	36.0
33	34.89644146279393	36.0	36.0	36.0	32.0	36.0
34	34.88322634537299	36.0	36.0	36.0	32.0	36.0
35	34.87098536723673	36.0	36.0	36.0	32.0	36.0
36	34.85134506533723	36.0	36.0	36.0	32.0	36.0
37	34.84155247972348	36.0	36.0	36.0	32.0	36.0
38	34.820798242906726	36.0	36.0	36.0	32.0	36.0
39	34.80526173040412	36.0	36.0	36.0	32.0	36.0
40	34.793372210301854	36.0	36.0	36.0	32.0	36.0
41	34.76539683992957	36.0	36.0	36.0	32.0	36.0
42	34.73425834725137	36.0	36.0	36.0	32.0	36.0
43	34.728810255486366	36.0	36.0	36.0	32.0	36.0
44	34.68036122403875	36.0	36.0	36.0	32.0	36.0
45	34.65923534742414	36.0	36.0	36.0	32.0	36.0
46	34.63858054271224	36.0	36.0	36.0	32.0	36.0
47	34.612927059659754	36.0	36.0	36.0	32.0	36.0
48	34.5853897812346	36.0	36.0	36.0	32.0	36.0
49	34.57914229472562	36.0	36.0	36.0	32.0	36.0
50	34.13494118000312	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	6.0
20	18.0
21	43.0
22	123.0
23	306.0
24	880.0
25	2086.0
26	4518.0
27	8650.0
28	15120.0
29	25019.0
30	37871.0
31	58099.0
32	90012.0
33	161225.0
34	368618.0
35	1258941.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.279837790036964	17.87923669143671	12.741685163775735	26.09924035475059
2	16.553256950170447	19.316538882803943	36.41537344474938	27.714830722276236
3	18.96575741988201	22.15679276999904	27.958071113714507	30.919378696404443
4	13.242042847361382	15.383869454506613	33.96005093680302	37.41403676132899
5	15.65559261563664	35.67271266667192	32.03221601783085	16.6394786998606
6	36.305914643811036	33.62818441630807	15.707933020072517	14.357967919808381
7	31.897179327770058	29.510661139816797	19.006889857285394	19.585269675127748
8	29.011876229672414	32.28023905053169	18.244068407319187	20.46381631247671
9	27.117989975077982	14.504092221800539	18.33877502600248	40.039142777119
10	17.38098660324011	25.846108560222415	29.352617920627544	27.42028691590993
11	37.77031872911987	21.152162131430536	21.158659674916088	19.918859464533504
12	25.14708082948075	23.04950539887061	27.316129273613658	24.487284498034985
13	29.731971408839712	19.02052485384219	24.58882117332837	26.65868256398973
14	24.22353124752343	18.938714874501425	24.14487159229687	32.69288228567828
15	25.88148776025246	26.569439788692012	21.337735911282934	26.211336539772596
16	26.591389920680314	25.37220788707602	22.5800703510563	25.456331841187364
17	24.611710246970645	25.604357685830976	24.370415109348244	25.413516957850142
18	25.52862736797545	24.514615795445316	25.12981563202209	24.826941204557144
19	26.248549743371647	24.42653025763252	24.717935238196496	24.606984760799335
20	26.442533460003055	23.75962944184925	24.232081239263017	25.565755858884682
21	27.11893857652535	23.603470477510612	24.143702105205126	25.13388884075891
22	26.56126863552079	23.51062274524166	24.525568571972848	25.402540047264704
23	25.114236167000648	23.637226395581276	25.06629217188759	26.182245265530486
24	25.268197932387636	24.242021499151868	24.690109189071052	25.799671379389444
25	25.718361454133404	23.71731101586831	24.68475776667177	25.87956976332651
26	25.162943933307307	24.736663603289546	24.917611235292465	25.182781228110677
27	25.74525038246998	23.985790034318928	24.594345548088832	25.67461403512226
28	25.54094078927614	23.957584197347092	24.890536926527943	25.61093808684883
29	25.238626155228218	24.203837018988196	25.09090461599045	25.466632209793133
30	25.235969447402013	24.38177850695516	25.04803070420913	25.3342213414337
31	25.96429879381431	23.822659594157695	24.3561988808424	25.85684273118559
32	25.733539418760337	24.071138851697253	24.145122863668583	26.05019886587383
33	25.328409629575297	23.890234615927973	24.58773743768868	26.19361831680805
34	25.937876568296197	24.061483511723335	24.580507840496498	25.42013207948397
35	26.131178899066764	23.970197845861076	24.876706287031798	25.021916968040358
36	25.312043600700363	24.409471299175447	24.541244098650917	25.737241001473276
37	26.206862263947546	23.92730054388731	24.499443018833002	25.366394173332147
38	25.471699506334367	23.802224621062773	24.747518750581456	25.978557122021407
39	26.185663936362918	23.845347326140033	24.13709931479877	25.831889422698275
40	26.098761676504047	24.04770378289935	24.540581835329604	25.312952705267
41	25.117806633203827	24.513772288256945	24.960780346806583	25.40764073173265
42	26.479113132685512	24.197113349282766	24.253280956178436	25.070492561853285
43	25.925247695095422	23.64276575750371	24.522589763166717	25.90939678423415
44	25.398272474834616	24.267411483656343	24.355131602329553	25.979184439179487
45	25.407180715039217	24.490553387720908	24.396877333594556	25.70538856364532
46	25.602949190070458	24.215286149508263	24.330965757359316	25.850798903061968
47	25.550256804557804	24.202143631374952	24.787620612491423	25.459978951575824
48	26.20577488132878	24.582700165841885	23.75634582734624	25.45517912548309
49	25.646335724398117	24.53643994541835	24.029364218302966	25.78786011188057
50	25.673160557795004	24.60119350292567	24.17850070317534	25.54714523610398
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	54.0
1	56.0
2	58.0
3	164.0
4	270.0
5	284.5
6	299.0
7	241.5
8	184.0
9	257.5
10	331.0
11	562.0
12	793.0
13	1560.0
14	2327.0
15	3083.0
16	3839.0
17	3664.0
18	3489.0
19	3301.5
20	3114.0
21	3550.0
22	3986.0
23	4797.0
24	5608.0
25	7199.5
26	8791.0
27	11052.0
28	13313.0
29	15841.0
30	18369.0
31	21255.5
32	24142.0
33	28142.5
34	32143.0
35	37673.0
36	43203.0
37	52500.0
38	61797.0
39	69692.5
40	77588.0
41	85937.5
42	94287.0
43	99572.5
44	104858.0
45	115723.0
46	126588.0
47	135425.0
48	144262.0
49	151099.5
50	157937.0
51	156727.5
52	155518.0
53	156326.0
54	157134.0
55	157736.0
56	158338.0
57	153530.0
58	148722.0
59	139047.0
60	129372.0
61	114513.0
62	99654.0
63	87549.0
64	75444.0
65	65019.5
66	54595.0
67	48650.0
68	42705.0
69	37051.0
70	31397.0
71	24339.0
72	17281.0
73	14651.0
74	12021.0
75	9118.0
76	6215.0
77	5444.5
78	4674.0
79	3819.5
80	2965.0
81	2255.5
82	1546.0
83	1373.5
84	1201.0
85	942.0
86	683.0
87	455.5
88	228.0
89	156.5
90	85.0
91	58.5
92	32.0
93	28.5
94	25.0
95	25.5
96	26.0
97	30.0
98	34.0
99	23.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02899282661354433
2	0.005513067199859023
3	9.844762856891111E-5
4	0.0
5	4.922381428445556E-5
6	1.4767144285336668E-4
7	0.0
8	0.0
9	0.0
10	4.922381428445556E-5
11	0.0
12	4.922381428445556E-5
13	0.0
14	0.0
15	0.0
16	5.414619571290112E-4
17	0.0
18	9.844762856891111E-5
19	0.0
20	3.4456669999118896E-4
21	4.922381428445556E-5
22	0.0
23	0.0
24	7.383572142668335E-4
25	1.9689525713782223E-4
26	4.922381428445556E-4
27	2.461190714222778E-4
28	0.001476714428533667
29	5.906857714134667E-4
30	0.0011813715428269334
31	4.4301432856010006E-4
32	8.368048428357446E-4
33	1.9689525713782223E-4
34	0.0016243858713870336
35	2.9534288570673336E-4
36	4.922381428445556E-4
37	0.0024611907142227784
38	0.0
39	8.368048428357446E-4
40	0.0
41	0.001476714428533667
42	0.005759186271281301
43	0.0053161719427212
44	0.0037410098856186227
45	0.0033472193713429783
46	0.0031503241142051557
47	0.0015259382428181225
48	0.004134800399894267
49	0.004184024214178722
50	0.0018705049428093114
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2031537.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.963447357482
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.27606686290353	33.680206418163976
2	14.402889161304937	13.240128755225752
3	4.737412161496093	6.53243383486862
4	2.1765307722354925	4.001634302863429
5	1.2003633917337977	2.7586419782902474
6	0.7839533166638851	2.161991820072234
7	0.5363861874839687	1.7257910804189904
8	0.3877687991419037	1.4258552628986323
9	0.305189725664211	1.2624814700650202
>10	1.9573280410761122	16.693797729922373
>50	0.14374580814646495	4.526019994083868
>100	0.08063327397084637	7.240281239074356
>500	0.008503370239674365	2.6235046566620728
>1k	0.0032291279391168477	2.127231457390419
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2210	0.10878462956864679	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	2098	0.10327156236878776	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	9.844762856891113E-5
13	0.0	0.0	0.0	0.0	9.844762856891113E-5
14	0.0	0.0	0.0	0.0	1.9689525713782226E-4
15	0.0	0.0	0.0	0.0	1.9689525713782226E-4
16	0.0	0.0	0.0	4.9223814284455564E-5	1.9689525713782226E-4
17	0.0	0.0	0.0	4.9223814284455564E-5	1.9689525713782226E-4
18	0.0	0.0	0.0	1.4767144285336668E-4	1.9689525713782226E-4
19	0.0	0.0	0.0	3.4456669999118896E-4	1.9689525713782226E-4
20	0.0	0.0	0.0	5.906857714134667E-4	2.461190714222778E-4
21	0.0	0.0	0.0	7.87581028551289E-4	2.461190714222778E-4
22	0.0	0.0	0.0	0.0010337000999735668	2.461190714222778E-4
23	0.0	0.0	0.0	0.0015259382428181225	2.461190714222778E-4
24	0.0	0.0	0.0	0.0020674001999471336	2.461190714222778E-4
25	0.0	0.0	0.0	0.0027565335999295117	2.461190714222778E-4
26	0.0	0.0	0.0	0.003150324114205156	2.461190714222778E-4
27	0.0	0.0	0.0	0.003593338442765256	2.461190714222778E-4
28	0.0	0.0	0.0	0.004134800399894267	2.461190714222778E-4
29	0.0	0.0	0.0	0.0055622910141434785	2.461190714222778E-4
30	0.0	0.0	0.0	0.008121929356935167	2.461190714222778E-4
31	0.0	0.0	0.0	0.011666043985415969	2.461190714222778E-4
32	0.0	0.0	0.0	0.01767134932811955	2.9534288570673336E-4
33	0.0	0.0	0.0	0.02382432611367649	2.9534288570673336E-4
34	0.0	0.0	0.0	0.030912555370638094	3.4456669999118896E-4
35	0.0	0.0	0.0	0.03923137998471108	3.4456669999118896E-4
36	0.0	0.0	0.0	0.04843623325590427	3.4456669999118896E-4
37	0.0	0.0	0.0	0.06625525402687718	3.4456669999118896E-4
38	0.0	0.0	0.0	0.08500952726925476	3.4456669999118896E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2885	0.0	29.519653	1
GGTATCA	1025	0.0	28.125067	1
TCAACGC	4385	0.0	19.314684	4
GGCGTTA	660	0.0	19.002127	42
TAGATCG	70	8.129224E-4	18.856005	5
ATCAACG	4545	0.0	18.68314	3
CAACGCA	4535	0.0	18.675829	5
AACGCAG	4575	0.0	18.560627	6
TATCAAC	4570	0.0	18.534166	2
CAACGCG	145	6.31735E-9	18.206245	21
AGCGCTA	170	1.2732926E-10	18.11789	14
ATTACTC	100	2.485652E-5	17.598936	3
TCGATCG	190	3.274181E-11	17.369509	41
CGTTATT	725	0.0	17.297634	44
AATCGGT	80	0.001989127	16.499815	19
ATACCGT	670	0.0	16.41692	6
TACCGTC	660	0.0	16.332346	7
CCTATTC	515	0.0	16.23203	3
GGACCGT	150	1.8406536E-7	16.132359	6
CCGCCTA	165	3.1113814E-8	16.001003	41
>>END_MODULE