FastQCFastQC Report
Thu 2 Feb 2017
SRR4062074_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062074_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1159369
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19940.1719901084124209No Hit
TATCAACGCAGAGTACTTTTTTTTT13540.11678766639439213No Hit
GCGCAAGACGGACCAGAGCGAAAGC11690.10083071049855569No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGT350.002168432316.2871956
GTGCTAC759.703799E-713.9285851
AATATAG551.9579622E-413.8194385
GGTATCA6550.013.19397451
AACCGCG604.0860017E-412.6683657
CGGTTCT1750.012.4868512
TGCTCGA701.0890725E-412.21592410
GCTCGTA701.0890725E-412.2159249
GTATTAG1103.8276994E-812.0867881
CTATAGA550.003075475612.0867881
TCTATAC802.8710856E-511.8724913
GTATCAA14300.011.4226791
ACTGCGC752.0703636E-411.4015298
GTATAGG1001.9359122E-611.3961151
CTGCGTG855.312648E-511.1779699
AATATTG600.005870152711.0843415
GATAGGT600.005883375611.0809923
CAAGACG2150.011.0475164
ACTCTAA1553.110472E-1011.03373710
CTCGTAG951.3572344E-511.00147510
Produced by FastQC (version 0.11.5)