##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062074_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1159369
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.223081693576418	32.0	32.0	32.0	32.0	32.0
2	30.80558476205591	32.0	32.0	32.0	32.0	32.0
3	30.83048968878761	32.0	32.0	32.0	32.0	32.0
4	30.83924790122903	32.0	32.0	32.0	32.0	32.0
5	30.713045630856094	32.0	32.0	32.0	32.0	32.0
6	34.393476968937414	36.0	36.0	36.0	32.0	36.0
7	34.3261800168885	36.0	36.0	36.0	32.0	36.0
8	34.26595329010867	36.0	36.0	36.0	32.0	36.0
9	34.410066165301984	36.0	36.0	36.0	32.0	36.0
10	34.05920461906434	36.0	36.0	36.0	32.0	36.0
11	34.39931635225713	36.0	36.0	36.0	32.0	36.0
12	34.19847175489426	36.0	36.0	36.0	32.0	36.0
13	34.31076128480234	36.0	36.0	36.0	32.0	36.0
14	34.19028626778877	36.0	36.0	36.0	32.0	36.0
15	34.13328457117622	36.0	36.0	36.0	32.0	36.0
16	34.14483481963033	36.0	36.0	36.0	32.0	36.0
17	34.063908039631905	36.0	36.0	36.0	32.0	36.0
18	34.06790762906374	36.0	36.0	36.0	32.0	36.0
19	34.07725236745161	36.0	36.0	36.0	32.0	36.0
20	34.067495335824916	36.0	36.0	36.0	32.0	36.0
21	34.04688067388381	36.0	36.0	36.0	32.0	36.0
22	34.02148151278842	36.0	36.0	36.0	32.0	36.0
23	33.979909761258064	36.0	36.0	36.0	32.0	36.0
24	33.942536845473704	36.0	36.0	36.0	32.0	36.0
25	33.59228597625088	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	17.0
5	95.0
6	247.0
7	47.0
8	204.0
9	139.0
10	89.0
11	21.0
12	43.0
13	57.0
14	144.0
15	232.0
16	372.0
17	519.0
18	634.0
19	836.0
20	1325.0
21	1941.0
22	2860.0
23	4359.0
24	6366.0
25	9231.0
26	12987.0
27	17305.0
28	23516.0
29	31568.0
30	42188.0
31	58284.0
32	83386.0
33	117463.0
34	247292.0
35	495602.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.967292186351735	17.475132746110773	11.678983849654463	25.878591217883027
2	16.792453806649497	19.146671542894996	37.22353424199088	26.837340408464627
3	19.509058414786036	22.36234694194403	27.978874624363016	30.149720018906915
4	13.191598382751652	15.237571091038799	34.59538168113432	36.975448845075235
5	15.428672502232557	35.86888527461529	33.01449112801287	15.687951095139283
6	35.487145140631846	34.07349036964409	16.32983149034893	14.109532999375125
7	31.32001771731557	29.75205255791585	19.755411249804652	19.172518474963933
8	28.48933697373488	33.022569453438955	18.706707629818826	19.781385943007336
9	26.99074349933549	14.736048538985852	18.636576778696316	39.63663118298234
10	17.027616435989895	26.322803532485477	30.444151750048583	26.205428281476046
11	37.23758662178702	21.24545351682512	21.662896464993267	19.8540633963946
12	24.554142465398485	23.456135426338175	28.107183728110897	23.88253838015244
13	29.613342119376767	19.31354861442771	24.90670586416581	26.166403402029715
14	23.692661877461635	19.406729238934354	24.666046643368297	32.23456224023572
15	25.397295625947898	26.75950141126316	21.79468798474385	26.0485149780451
16	26.134359730769297	25.728561978503688	23.09083340961652	25.046244881110496
17	24.386412468996774	25.95738654028174	24.748433430286784	24.907767560434703
18	25.02922916180953	25.0907960521203	25.36055056170073	24.519424224369434
19	25.779169145255832	24.986164508099698	24.999633068077525	24.235033278566945
20	25.861114828260508	24.42667886850932	24.586137382963752	25.126068920266427
21	26.50918408575267	24.281538243714106	24.364935751112828	24.844341919420394
22	26.094550666327066	24.000552574435662	24.776919656540453	25.127977102696825
23	24.729182510572436	24.289452080691483	25.192651823103024	25.788713585633054
24	24.991366538776326	24.666316723705066	25.054218136484664	25.288098601033944
25	25.20201531863168	24.377723753548217	24.884575008115146	25.535685919704953
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	469.0
1	469.0
2	409.0
3	349.0
4	349.0
5	349.0
6	778.5
7	1208.0
8	1208.0
9	1208.0
10	1520.0
11	1832.0
12	1832.0
13	1832.0
14	2390.5
15	2949.0
16	2949.0
17	2949.0
18	5057.5
19	7166.0
20	7166.0
21	7166.0
22	11023.0
23	14880.0
24	14880.0
25	14880.0
26	21521.5
27	28163.0
28	28163.0
29	28163.0
30	35451.5
31	42740.0
32	42740.0
33	42740.0
34	54142.0
35	65544.0
36	65544.0
37	65544.0
38	77736.0
39	89928.0
40	89928.0
41	89928.0
42	104834.5
43	119741.0
44	119741.0
45	119741.0
46	134171.5
47	148602.0
48	148602.0
49	148602.0
50	154225.0
51	159848.0
52	159848.0
53	159848.0
54	151710.5
55	143573.0
56	143573.0
57	143573.0
58	132200.5
59	120828.0
60	120828.0
61	120828.0
62	106487.5
63	92147.0
64	92147.0
65	92147.0
66	75009.5
67	57872.0
68	57872.0
69	57872.0
70	44283.0
71	30694.0
72	30694.0
73	30694.0
74	23165.5
75	15637.0
76	15637.0
77	15637.0
78	12243.5
79	8850.0
80	8850.0
81	8850.0
82	6206.0
83	3562.0
84	3562.0
85	3562.0
86	2636.0
87	1710.0
88	1710.0
89	1710.0
90	1114.0
91	518.0
92	518.0
93	518.0
94	318.0
95	118.0
96	118.0
97	118.0
98	279.5
99	441.0
100	441.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011212996034912093
2	2.5876144695950986E-4
3	4.3126907826584977E-4
4	0.010005442615767716
5	0.03312146521081726
6	0.06391407739899893
7	0.10177950247074055
8	0.1082485386447283
9	0.11834023507614917
10	0.1331758913684944
11	0.13852362793899095
12	0.1331758913684944
13	0.1269656166414662
14	0.1162701435004731
15	0.1329171299215349
16	0.12101410336139745
17	0.12291168730576718
18	0.11031863022040439
19	0.09625925826893768
20	0.09263659801150453
21	0.09151529840801333
22	0.09953690326375814
23	0.0803885561887544
24	0.09358538998368941
25	0.08979022209494993
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1159369.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.975684765085845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.59529240652851	46.94186873752257
2	12.596713229330755	14.857995769783944
3	3.5595152993898753	6.2977455663995245
4	1.4528296659881086	3.4272649759471863
5	0.7401200849737156	2.1824544409859206
6	0.45574222252919605	1.6126625790012883
7	0.28993752078571067	1.1969484679200018
8	0.21448411827000863	1.0119478196967533
9	0.14446750889883292	0.7668063237253323
>10	0.8050875449408681	8.610461844054678
>50	0.07601657676421737	3.1645947241276966
>100	0.06465112238860549	7.578372020421289
>500	0.004701896422012024	1.959453891707015
>1k	4.408027895636273E-4	0.39142283870690725
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1994	0.1719901084124209	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1354	0.11678766639439213	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1169	0.10083071049855569	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	8.625381565316995E-5
6	8.625381565316995E-5	0.0	0.0	0.0	8.625381565316995E-5
7	8.625381565316995E-5	0.0	0.0	0.0	8.625381565316995E-5
8	8.625381565316995E-5	0.0	0.0	0.0	8.625381565316995E-5
9	8.625381565316995E-5	0.0	0.0	8.625381565316995E-5	1.725076313063399E-4
10	8.625381565316995E-5	0.0	0.0	8.625381565316995E-5	1.725076313063399E-4
11	8.625381565316995E-5	0.0	0.0	8.625381565316995E-5	1.725076313063399E-4
12	8.625381565316995E-5	0.0	0.0	8.625381565316995E-5	6.037767095721897E-4
13	8.625381565316995E-5	0.0	0.0	8.625381565316995E-5	6.900305252253596E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCACCGT	35	0.0021684323	16.287195	6
GTGCTAC	75	9.703799E-7	13.928585	1
AATATAG	55	1.9579622E-4	13.819438	5
GGTATCA	655	0.0	13.1939745	1
AACCGCG	60	4.0860017E-4	12.668365	7
CGGTTCT	175	0.0	12.48685	12
TGCTCGA	70	1.0890725E-4	12.215924	10
GCTCGTA	70	1.0890725E-4	12.215924	9
GTATTAG	110	3.8276994E-8	12.086788	1
CTATAGA	55	0.0030754756	12.086788	1
TCTATAC	80	2.8710856E-5	11.872491	3
GTATCAA	1430	0.0	11.422679	1
ACTGCGC	75	2.0703636E-4	11.401529	8
GTATAGG	100	1.9359122E-6	11.396115	1
CTGCGTG	85	5.312648E-5	11.177969	9
AATATTG	60	0.0058701527	11.084341	5
GATAGGT	60	0.0058833756	11.080992	3
CAAGACG	215	0.0	11.047516	4
ACTCTAA	155	3.110472E-10	11.033737	10
CTCGTAG	95	1.3572344E-5	11.001475	10
>>END_MODULE