FastQCFastQC Report
Thu 2 Feb 2017
SRR4062074_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062074_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1159369
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13700.023.4522461
CTTAGCG500.0025810821.9989643
CTAGCGG2500.021.11991729
GTATTAG2000.020.9062291
CAATACG2550.020.7084838
CGCAATA2550.020.70669436
TAGCGGC2550.020.70580330
ATACGAA2650.019.92702940
AATAGCG2500.019.3590895
TACGAAT2850.019.30066741
TCTAGCG2850.018.52624328
CGCCGGT2850.018.5254447
CGTCGTA4050.018.46826710
GTCCTAT2300.018.179331
ATACCGT4250.018.1167966
GGTATCA8550.018.0170651
GTACTAA1102.788278E-618.0053651
AATACGA2950.017.9005539
ATAGCGT2600.017.7683946
CCGTCGT4150.017.4931539
Produced by FastQC (version 0.11.5)