FastQCFastQC Report
Thu 2 Feb 2017
SRR4062073_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062073_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2285130
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTAAGTTGGTTGTTAAGCGTAC109480.4790974692905874No Hit
GTATCAACGCAGAGTACTTTTTTTT78250.3424312839969717No Hit
ATGTAGATAAGGGAAGTCGGCAAAA73940.323570212635605No Hit
GTATCAACGCAGAGTACATGGGCAG65040.28462275669217946No Hit
ACTAGAGGCTGTTCACCTTGGAGAC64730.28326615991212756No Hit
CCCCTTGTCCGTACCAGTTCTAAGT61920.27096926651875386No Hit
GCCTCTAGTTGATAGAACAATGTAG59460.2602040146512452No Hit
TATCTACATTGTTCTATCAACTAGA59370.25981016397316564No Hit
GTCCGTACCAGTTCTAAGTTGGTTG57720.25258956820837325No Hit
CCCGTGAAGGCAGCCAAGACCATCC52860.23132163159207572No Hit
GTTCTTGACAGCCCTTGAAAATCCA52510.2297899900662107No Hit
ATCCTAAGAGATAGGGAAGCTCCGT51060.22344461803048402No Hit
AGCCAAGACCATCCACACCCAGGTT50990.22313828972531105No Hit
GCTTAACCCCCTGAAATCGGTTTGT47470.207734352093754No Hit
GTCAAGAACGCACCGGACACGACAG46950.20545877039818303No Hit
TCGCAATGCTATGTTTTAATTAGAC46500.20348951700778511No Hit
TATCAACGCAGAGTACTTTTTTTTT46240.20235172615999963No Hit
GCCAAGACCATCCACACCCAGGTTC45160.19762551802304465No Hit
CCCTTAGAGCCAATCCTTATCCCGA43940.19228665327574362No Hit
CTTCTGTCGTGTCCGGTGCGTTCTT43250.1892671314104668No Hit
CTCCTTAACTGCTTAACCCCCTGAA42980.18808557937622805No Hit
CTTTCACACCTGGTCGTACTCATAA42770.18716659446070902No Hit
GTTTTAATTAGACAGTCAGATTCCC42110.18427835615479207No Hit
GTACATGGGCAGAAATCACATTGCG41200.18029608818754295No Hit
GTTCCAGCTAGATCCCGAGGCTTCC40230.17605125310157407No Hit
GTCAGATTCCCCTTGTCCGTACCAG39600.173294298355017No Hit
ACGCAGAGTACATGGGCAGAAATCA39070.17097495547299277No Hit
GTCGTACTCATAACCGCAGCAGGTC36940.16165382275844262No Hit
TATCAACGCAGAGTACATGGGCAGA36410.1593344798764184No Hit
AGATAGGGAAGCTCCGTTTCAAAGT36290.15880934563897897No Hit
GTTCTATCAACTAGAGGCTGTTCAC34970.15303286902714508No Hit
GGGTTAGTCGATCCTAAGAGATAGG34500.15097609326384057No Hit
CCTTGGAGACCTGCTGCGGTTATGA34350.15031967546704125No Hit
GGATTGGCTCTAAGGGTTGGGTACG33910.1483941832630966No Hit
AGATAAGGGAAGTCGGCAAAATAGA33320.1458122732623527No Hit
GTCCGGAGCTAGGGGTCGATGGCAG32470.14209257241382328No Hit
TGATAGAACAATGTAGATAAGGGAA32370.14165496054929042No Hit
TCTCCAAGGTGAACAGCCTCTAGTT32120.14056093088795824No Hit
GAGTACGACCAGGTGTGAAAGTTAT31900.13959818478598593No Hit
GGTATCAACGCAGAGTACTTTTTTT31090.13605352868326967No Hit
GTAGAGCACGGCACTTCTGTCGTGT30660.1341717976657783No Hit
GCTCTAAGGGTTGGGTACGTCGGGC30400.13303400681799285No Hit
GGGTTAAGCAGTTAAGGAGAAAAGA30160.13198373834311397No Hit
CGACAGAAGTGCCGTGCTCTACCTG29120.1274325749519721No Hit
GTTGTTAAGCGTACGCCGGACGCCC28380.12419424715442884No Hit
ACATTGTTCTATCAACTAGAGGCTG27770.12152481478077833No Hit
GAACAATGTAGATAAGGGAAGTCGG27470.12021197918717973No Hit
CTCTAAGGGTTGGGTACGTCGGGCC27450.12012445681427314No Hit
AAATCACATTGCGTCAACACCACTT27080.11850529291550153No Hit
CCCAGGTTCCGGCCGGATCCGTCGG26910.11776135274579565No Hit
ATCGAAAGGGAATCCGGTTAAGATT26720.11692989020318319No Hit
AGCTGGAACTGTTGGGGCAACCCGA26700.11684236783027661No Hit
GGATAAGGATTGGCTCTAAGGGTTG26490.11592338291475758No Hit
GGTATCAACGCAGAGTACATGGGCA26400.115529532236678No Hit
CCCTTATCTACATTGTTCTATCAAC26230.11478559206697211No Hit
GAGATAGGGAAGCTCCGTTTCAAAG25690.11242248799849461No Hit
GCGTACGCCGGACGCCCGTGAAGGC25650.11224744325268148No Hit
GTTAAGGAGAAAAGAGAACTCTTCC25580.11194111494750845No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25200.11027818986228353No Hit
TGATTGGACCGCTCGGCGATTGAGA24990.10935920494676453No Hit
GGTAACAGCAGTTGGACATGGGTTA24940.10914039901449808No Hit
GCTATGTTTTAATTAGACAGTCAGA24870.10883407070932506No Hit
CTAGTTGATAGAACAATGTAGATAA24330.10647096664084757No Hit
CTTTCTGGCCATCGCAATGCTATGT24330.10647096664084757No Hit
TCCTTATCCCGAAGTTACGGATCTA24290.10629592189503442No Hit
GGCAAGGGCCCCGGGAAGAGTTCTC23880.10450171325044964No Hit
TCCACACCCAGGTTCCGGCCGGATC23750.10393281782655692No Hit
GAGCTAGGGGTCGATGGCAGGTAGA23630.10340768358911748No Hit
CCCCAACAGTTCCAGCTAGATCCCG23110.10113210189354653No Hit
GGGTTGGGTACGTCGGGCCTTGACT23060.1009132959612801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAAA902.2955646E-914.7751713
TTACGCT400.005282448614.2490484
TTTAGAA903.652167E-813.71922
GTCCAAT4250.013.4073431
TGGTTGT16300.013.40694910
TAGGACC3700.013.3504594
GGTATCA21950.013.2393681
TAAGGTT3300.012.9536794
TAACCTA3450.012.6672085
GTATCAA58750.012.5116181
TGGACCG5250.012.4862485
TCCTGTA1600.012.4660052
ATTGGAC4450.012.3798383
ACCTATT3100.012.2599317
TGATTGG4250.012.0666081
ACACAGT951.038834E-612.0015646
CTATTCT3100.011.9534329
GACGGCT3900.011.935883513
ACGGCTC3900.011.93562114
AGGACCT3350.011.9109565
Produced by FastQC (version 0.11.5)