FastQCFastQC Report
Thu 2 Feb 2017
SRR4062073_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062073_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2285130
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGCCCGTGAAGGCAGCCAA96620.42282058351166013No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGATAAGGA73980.32374525738141813No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAG66160.2895240095749476No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTG61420.2687812071960895No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGC58710.2569219256672487No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATC54910.2402926748149996No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTT54350.2378420483736155No Hit
AGCCAAGACCATCCACACCCAGGTTCCGGCCGGATCCGTCGGGTTGCCCC51440.22510754311570896No Hit
CCCGTGAAGGCAGCCAAGACCATCCACACCCAGGTTCCGGCCGGATCCGT50580.22134408108072626No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGCCCGTGA50040.21898097701224875No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA47700.20874085938217957No Hit
GCTTAACCCCCTGAAATCGGTTTGTCCGGAGCTAGGGGTCGATGGCAGGT47350.20720921785631455No Hit
GTCAAGAACGCACCGGACACGACAGAAGTGCCGTGCTCTACCTGCCATCG45370.19854450293856368No Hit
GCCAAGACCATCCACACCCAGGTTCCGGCCGGATCCGTCGGGTTGCCCCA44250.19364325005579552No Hit
CTTTCACACCTGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACA42190.18462844564641837No Hit
CTCCTTAACTGCTTAACCCCCTGAAATCGGTTTGTCCGGAGCTAGGGGTC40120.17556988005058793No Hit
CTTCTGTCGTGTCCGGTGCGTTCTTGACAGCCCTTGAAAATCCAAGGGAA39800.17416952208408276No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGG39580.17320677598211043No Hit
CCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTATTTTGCCGACTT39270.17185017920205853No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGC38120.1668176427599305No Hit
GTTCCAGCTAGATCCCGAGGCTTCCAGTCAAGGCCCGACGTACCCAACCC37850.16563609072569174No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACG37260.16305418072494782No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCA35350.15469579411236997No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTT33820.14800033258501705No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTC33020.1444994376687541No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAG32640.14283651258352917No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAG32100.14047340851505166No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGTGTGAAAGTTATTC32020.14012331902342537No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGG32000.14003579665051877No Hit
GGATTGGCTCTAAGGGTTGGGTACGTCGGGCCTTGACTGGAAGCCTCGGG31320.13706003597169528No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGA30380.13294648444508628No Hit
ACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTT29780.13032081325788905No Hit
GAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAT29590.1294893507152766No Hit
CGACAGAAGTGCCGTGCTCTACCTGCCATCGACCCCTAGCTCCGGACAAA29340.1283953210539444No Hit
GTAGAGCACGGCACTTCTGTCGTGTCCGGTGCGTTCTTGACAGCCCTTGA28860.12629478410418662No Hit
GTCCGGAGCTAGGGGTCGATGGCAGGTAGAGCACGGCACTTCTGTCGTGT28380.12419424715442884No Hit
GGGTTAGTCGATCCTAAGAGATAGGGAAGCTCCGTTTCAAAGTGCACGAT27670.12108720291624546No Hit
GCTCTAAGGGTTGGGTACGTCGGGCCTTGACTGGAAGCCTCGGGATCTAG27330.1195993225768337No Hit
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT27330.1195993225768337No Hit
ATCCTAAGAGATAGGGAAGCTCCGTTTCAAAGTGCACGATTTTCCGTGCC27320.11955556139038041No Hit
ATCGAAAGGGAATCCGGTTAAGATTCCGGAACCAGGATGTGGATCTTTAA26650.11662356189801018No Hit
CTCTAAGGGTTGGGTACGTCGGGCCTTGACTGGAAGCCTCGGGATCTAGC26210.11469806969406555No Hit
CCCTTATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCT26070.11408541308371951No Hit
TGATTGGACCGCTCGGCGATTGAGAGTTTCTAGTGGGCCATTTTTGGTAA26010.1138228459649998No Hit
AGCTGGAACTGTTGGGGCAACCCGACGGATCCGGCCGGAACCTGGGTGTG25550.1118098313881486No Hit
GTTCTTGACAGCCCTTGAAAATCCAAGGGAATGAATAACTTTCACACCTG25160.1101031451164704No Hit
GCGTACGCCGGACGCCCGTGAAGGCAGCCAAGACCATCCACACCCAGGTT25090.10979681681129738No Hit
CCCAGGTTCCGGCCGGATCCGTCGGGTTGCCCCAACAGTTCCAGCTAGAT24810.10857150359060534No Hit
GGTAACAGCAGTTGGACATGGGTTAGTCGATCCTAAGAGATAGGGAAGCT24610.10769627986153961No Hit
CTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTA24250.10612087714922126No Hit
GGATAAGGATTGGCTCTAAGGGTTGGGTACGTCGGGCCTTGACTGGAAGC24210.10594583240340812No Hit
TCCACACCCAGGTTCCGGCCGGATCCGTCGGGTTGCCCCAACAGTTCCAG24170.10577078765759498No Hit
GTTAAGGAGAAAAGAGAACTCTTCCCGGGGCCCTTGCCAGCGTCTCCAAG24130.10559574291178182No Hit
GTTGTTAAGCGTACGCCGGACGCCCGTGAAGGCAGCCAAGACCATCCACA23990.1049830863014358No Hit
TCCTTATCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACAT23570.10314511647039776No Hit
GCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAA23230.10165723613098597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63500.026.027881
GTCCTAC6600.026.0089741
GGTATCA28350.024.0647431
CCTACAG7300.023.8075793
GTCCTAA2900.023.5253621
TCCTACA8300.023.3267172
CTACAGT8550.021.870284
TACAGTG9500.021.0726575
GTCCTAG2100.020.9596161
CCTGTAC1900.020.8415453
ATTTCTA4400.020.49944715
TAGGACG5600.020.4275764
TTAACGC1101.2672353E-719.9994623
CTGTAGG6200.019.8778291
GTAGGAC7900.019.7716223
TGTAGGA7400.019.6228122
GTGTGCA6750.019.5546029
GTTCTTA3900.019.17897221
TCCTAGA2200.019.0011522
TAGGACC4650.018.9238074
Produced by FastQC (version 0.11.5)