FastQCFastQC Report
Thu 2 Feb 2017
SRR4062072_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062072_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841209
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15780.18758715135002124No Hit
GCGCAAGACGGACCAGAGCGAAAGC10500.12482034785647801No Hit
TATCAACGCAGAGTACTTTTTTTTT10400.12363158263879725No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG8650.10282819132938426No Hit
GAATAGGACCGCGGTTCTATTTTGT8640.10270931480761618No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGGT250.00603353418.99797417
TCGACGC350.002167177416.28785714
AACCGCG400.005273908414.2510277
TCCGATA602.563747E-514.2510278
GGTATCA5350.013.6649331
AATTGCA852.6897033E-713.41352914
TCTATAC500.00150042413.29779153
TTACACA1153.765308E-1013.216774
CGATAAC655.4369113E-513.15636110
CCGATAA655.4399497E-513.1555789
TAGAACC655.4490745E-513.1532294
AATTCCG655.4490745E-513.1532295
TCCAACG1102.743036E-912.95316518
TTAGGAC1251.0186341E-1012.9178553
CGGTCCA1550.012.87342810
GTATAGG751.4855421E-512.6592891
CGAACGA701.089017E-412.21516616
TTCCGAT701.089017E-412.2151667
TGTTACA701.0926544E-412.2108042
GGCTAAA550.003079839312.0838661
Produced by FastQC (version 0.11.5)