##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062072_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	841209
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.218461761583626	32.0	32.0	32.0	32.0	32.0
2	30.71740316615728	32.0	32.0	32.0	32.0	32.0
3	30.75894100039348	32.0	32.0	32.0	32.0	32.0
4	30.777969565232898	32.0	32.0	32.0	32.0	32.0
5	30.677958747469415	32.0	32.0	32.0	32.0	32.0
6	34.34573809837983	36.0	36.0	36.0	32.0	36.0
7	34.25413066194014	36.0	36.0	36.0	32.0	36.0
8	34.19049130477681	36.0	36.0	36.0	32.0	36.0
9	34.344148719283794	36.0	36.0	36.0	32.0	36.0
10	34.0115643080376	36.0	36.0	36.0	32.0	36.0
11	34.34044928192637	36.0	36.0	36.0	32.0	36.0
12	34.13643933909409	36.0	36.0	36.0	32.0	36.0
13	34.23514727017899	36.0	36.0	36.0	32.0	36.0
14	34.13077130653619	36.0	36.0	36.0	32.0	36.0
15	34.07921574781059	36.0	36.0	36.0	32.0	36.0
16	34.09197951995283	36.0	36.0	36.0	32.0	36.0
17	34.01407616894256	36.0	36.0	36.0	32.0	36.0
18	34.02068926984852	36.0	36.0	36.0	32.0	36.0
19	34.012187221011665	36.0	36.0	36.0	32.0	36.0
20	33.990206952136745	36.0	36.0	36.0	32.0	36.0
21	33.95886753470303	36.0	36.0	36.0	32.0	36.0
22	33.93475224349716	36.0	36.0	36.0	32.0	36.0
23	33.885696658024344	36.0	36.0	36.0	32.0	36.0
24	33.87867105558785	36.0	36.0	36.0	32.0	36.0
25	33.51393648902948	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	1.0
4	10.0
5	75.0
6	175.0
7	50.0
8	143.0
9	120.0
10	61.0
11	22.0
12	39.0
13	40.0
14	137.0
15	195.0
16	301.0
17	379.0
18	545.0
19	732.0
20	1205.0
21	1790.0
22	2625.0
23	3903.0
24	5457.0
25	7763.0
26	10469.0
27	13310.0
28	17950.0
29	23606.0
30	30670.0
31	41216.0
32	57978.0
33	81258.0
34	174042.0
35	364941.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.599511175674806	17.24508500930811	11.679358822256724	25.47604499276036
2	16.707401736550295	19.30557008492549	37.88611140169851	26.100916776825706
3	19.85515877969132	22.483556170552202	28.025539796266514	29.635745253489965
4	12.999380585578463	15.527685209073173	34.563800279865944	36.90913392548242
5	15.087861998927366	36.29865350015043	33.039369928923165	15.574114571999035
6	35.17186907094231	34.54058612842867	16.307726346516883	13.979818454112142
7	30.91333835540307	30.01936233995128	19.378524820568114	19.68877448407754
8	28.218121663998858	33.740665147304725	18.501187125490173	19.540026063206252
9	26.839678447559674	14.63637294179539	18.322038054022144	40.20191055662279
10	16.877562292576314	26.650223553331397	30.12529938052634	26.34691477356595
11	37.63171272073153	21.100464171639935	21.48356351437099	19.78425959325755
12	24.62233491661012	23.560468078521005	28.41862790614621	23.398569098722668
13	29.84108656318446	19.248047804970955	24.764070088562995	26.14679554328159
14	23.66896653506992	19.75248544084514	24.602561554309545	31.975986469775396
15	25.651638739292125	27.031341513935114	21.719307267783762	25.597712478988992
16	25.89213979048805	25.777754501317034	23.481359720904653	24.848745987290258
17	24.10639997238467	25.94817601917837	24.88534280507503	25.06008120336193
18	24.779102317521374	25.27029021385181	25.840030087787785	24.11057738083903
19	25.907101997879433	24.807191366361987	24.612865491508803	24.672841144249773
20	25.85590195145169	24.60506901475488	24.50368871965731	25.03534031413612
21	26.863540468084956	24.344034945139413	24.023474452137844	24.768950134637787
22	25.971526869538415	24.349608057112867	24.788736417069597	24.89012865627912
23	24.85124730960191	24.455168360323572	24.975817649011468	25.717766681063047
24	25.018472928384533	24.8247600304134	25.057977032884786	25.09879000831728
25	25.23339354089474	24.51447242231916	25.02207213960539	25.23006189718072
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	842.0
1	842.0
2	567.0
3	292.0
4	292.0
5	292.0
6	650.0
7	1008.0
8	1008.0
9	1008.0
10	1124.5
11	1241.0
12	1241.0
13	1241.0
14	1600.5
15	1960.0
16	1960.0
17	1960.0
18	3366.5
19	4773.0
20	4773.0
21	4773.0
22	7892.5
23	11012.0
24	11012.0
25	11012.0
26	17027.5
27	23043.0
28	23043.0
29	23043.0
30	30228.5
31	37414.0
32	37414.0
33	37414.0
34	43650.0
35	49886.0
36	49886.0
37	49886.0
38	57474.5
39	65063.0
40	65063.0
41	65063.0
42	74771.5
43	84480.0
44	84480.0
45	84480.0
46	93934.0
47	103388.0
48	103388.0
49	103388.0
50	107873.5
51	112359.0
52	112359.0
53	112359.0
54	106142.0
55	99925.0
56	99925.0
57	99925.0
58	92979.5
59	86034.0
60	86034.0
61	86034.0
62	76381.5
63	66729.0
64	66729.0
65	66729.0
66	55146.0
67	43563.0
68	43563.0
69	43563.0
70	33338.5
71	23114.0
72	23114.0
73	23114.0
74	17824.5
75	12535.0
76	12535.0
77	12535.0
78	9928.5
79	7322.0
80	7322.0
81	7322.0
82	5149.5
83	2977.0
84	2977.0
85	2977.0
86	2175.5
87	1374.0
88	1374.0
89	1374.0
90	906.0
91	438.0
92	438.0
93	438.0
94	286.5
95	135.0
96	135.0
97	135.0
98	218.5
99	302.0
100	302.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.321356523765201E-4
2	1.188765217680743E-4
3	0.0014265182612168915
4	0.010936640002662834
5	0.034236438269205395
6	0.06419332175476011
7	0.10912864698309219
8	0.11221943654906212
9	0.1230372000299569
10	0.13563811133737275
11	0.14479160351351447
12	0.1401554191645596
13	0.13421159307615585
14	0.12077854611636347
15	0.13944216003395113
16	0.1267223722047672
17	0.1302886678578094
18	0.11899539828984236
19	0.10318482089468847
20	0.09617110611037209
21	0.09581447654506788
22	0.10793988176541144
23	0.08559109567301348
24	0.09391245219677868
25	0.09308031654440217
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	841209.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.903792157723
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.07803799118506	38.661053331798385
2	15.432696469500309	16.328963329113396
3	5.334891833677564	8.467080262584346
4	2.314518996112742	4.897873276618007
5	1.1867057233029454	3.139061646899968
6	0.6375176948660373	2.02362621756381
7	0.43990858742912536	1.6290982734423574
8	0.2775208077412455	1.1745522505749002
9	0.19051543526883694	0.9071090091270609
>10	0.9396016315923938	9.020022810865106
>50	0.08426938191413993	3.122854184409683
>100	0.07703791797654311	7.997255521834711
>500	0.006099776489779447	2.1930541828111676
>1k	6.777529433088274E-4	0.4383957023571509
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1578	0.18758715135002124	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1050	0.12482034785647801	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1040	0.12363158263879725	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	865	0.10282819132938426	No Hit
GAATAGGACCGCGGTTCTATTTTGT	864	0.10270931480761618	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.3213565237652E-4
2	0.0	0.0	0.0	0.0	8.3213565237652E-4
3	0.0	0.0	0.0	0.0	8.3213565237652E-4
4	0.0	0.0	0.0	0.0	8.3213565237652E-4
5	0.0	0.0	0.0	0.0	9.510121741445942E-4
6	0.0	0.0	0.0	1.1887652176807428E-4	9.510121741445942E-4
7	0.0	0.0	0.0	1.1887652176807428E-4	9.510121741445942E-4
8	0.0	0.0	0.0	1.1887652176807428E-4	9.510121741445942E-4
9	0.0	0.0	0.0	1.1887652176807428E-4	9.510121741445942E-4
10	0.0	0.0	0.0	2.3775304353614856E-4	0.0010698886959126686
11	0.0	0.0	0.0	2.3775304353614856E-4	0.0010698886959126686
12	0.0	0.0	0.0	2.3775304353614856E-4	0.0010698886959126686
13	0.0	0.0	0.0	2.3775304353614856E-4	0.001307641739448817
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGGT	25	0.006033534	18.997974	17
TCGACGC	35	0.0021671774	16.287857	14
AACCGCG	40	0.0052739084	14.251027	7
TCCGATA	60	2.563747E-5	14.251027	8
GGTATCA	535	0.0	13.664933	1
AATTGCA	85	2.6897033E-7	13.413529	14
TCTATAC	50	0.001500424	13.2977915	3
TTACACA	115	3.765308E-10	13.21677	4
CGATAAC	65	5.4369113E-5	13.156361	10
CCGATAA	65	5.4399497E-5	13.155578	9
TAGAACC	65	5.4490745E-5	13.153229	4
AATTCCG	65	5.4490745E-5	13.153229	5
TCCAACG	110	2.743036E-9	12.953165	18
TTAGGAC	125	1.0186341E-10	12.917855	3
CGGTCCA	155	0.0	12.873428	10
GTATAGG	75	1.4855421E-5	12.659289	1
CGAACGA	70	1.089017E-4	12.215166	16
TTCCGAT	70	1.089017E-4	12.215166	7
TGTTACA	70	1.0926544E-4	12.210804	2
GGCTAAA	55	0.0030798393	12.083866	1
>>END_MODULE