FastQCFastQC Report
Thu 2 Feb 2017
SRR4062072_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062072_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841209
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10210.12137292872520386No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9010.10710774611303493No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8940.1062756104606584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG1850.026.1611734
AGGACGT2250.022.4880375
GTATCAA13700.022.3256321
GTGTTAT602.8678903E-422.00441
TATACTA602.8725062E-421.9991685
GTCCTAG1451.2732926E-1121.2456281
GGTATCA6900.021.0476861
CGCAATA3050.020.91848436
TCTATAG654.9317075E-420.3069233
ATACGAA3150.020.2556140
CGAATGC3100.019.8725843
GATATAC3700.019.6255461
TACGAAT3150.019.5571441
CTAGCGG3150.019.55597929
TGTAGGA3050.019.4746722
GGACGTG2500.019.3592686
TAGCGGC3300.019.33375230
GCGCAAT3300.019.33375235
TATGGCG1901.8189894E-1218.52561616
GGACCGT600.00741189118.332646
Produced by FastQC (version 0.11.5)