##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062072_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	841209
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25899746674132	32.0	32.0	32.0	32.0	32.0
2	31.445939118578142	32.0	32.0	32.0	32.0	32.0
3	31.57479294681821	32.0	32.0	32.0	32.0	32.0
4	31.65641356666417	32.0	32.0	32.0	32.0	32.0
5	31.627685866413696	32.0	32.0	32.0	32.0	32.0
6	35.28131891123371	36.0	36.0	36.0	36.0	36.0
7	35.27685509784132	36.0	36.0	36.0	36.0	36.0
8	35.23385745991781	36.0	36.0	36.0	36.0	36.0
9	35.31323131350235	36.0	36.0	36.0	36.0	36.0
10	35.192330324568566	36.0	36.0	36.0	36.0	36.0
11	35.312373025015184	36.0	36.0	36.0	36.0	36.0
12	35.23587479449221	36.0	36.0	36.0	36.0	36.0
13	35.27696089794569	36.0	36.0	36.0	36.0	36.0
14	35.23657378844021	36.0	36.0	36.0	36.0	36.0
15	35.206002313337116	36.0	36.0	36.0	36.0	36.0
16	35.22075370092331	36.0	36.0	36.0	36.0	36.0
17	35.2029626406755	36.0	36.0	36.0	36.0	36.0
18	35.19516552961274	36.0	36.0	36.0	36.0	36.0
19	35.18617727580185	36.0	36.0	36.0	36.0	36.0
20	35.18028813291346	36.0	36.0	36.0	36.0	36.0
21	35.16072343496087	36.0	36.0	36.0	36.0	36.0
22	35.153028557706826	36.0	36.0	36.0	36.0	36.0
23	35.114784791888816	36.0	36.0	36.0	36.0	36.0
24	35.10097015129415	36.0	36.0	36.0	36.0	36.0
25	35.07938693000194	36.0	36.0	36.0	36.0	36.0
26	35.043215181958345	36.0	36.0	36.0	36.0	36.0
27	35.03377757489518	36.0	36.0	36.0	36.0	36.0
28	35.006384857984166	36.0	36.0	36.0	36.0	36.0
29	34.98280094483059	36.0	36.0	36.0	36.0	36.0
30	34.966306827435275	36.0	36.0	36.0	36.0	36.0
31	34.96556147164379	36.0	36.0	36.0	36.0	36.0
32	34.941144234072624	36.0	36.0	36.0	36.0	36.0
33	34.91843644088449	36.0	36.0	36.0	32.0	36.0
34	34.89681161280966	36.0	36.0	36.0	32.0	36.0
35	34.88564791865042	36.0	36.0	36.0	32.0	36.0
36	34.845270319266675	36.0	36.0	36.0	32.0	36.0
37	34.84370947053586	36.0	36.0	36.0	32.0	36.0
38	34.81948837922562	36.0	36.0	36.0	32.0	36.0
39	34.78744045772216	36.0	36.0	36.0	32.0	36.0
40	34.78448756492144	36.0	36.0	36.0	32.0	36.0
41	34.7598028551763	36.0	36.0	36.0	32.0	36.0
42	34.720111173323154	36.0	36.0	36.0	32.0	36.0
43	34.72410304692413	36.0	36.0	36.0	32.0	36.0
44	34.6710662867373	36.0	36.0	36.0	32.0	36.0
45	34.64597501928772	36.0	36.0	36.0	32.0	36.0
46	34.60629284755632	36.0	36.0	36.0	32.0	36.0
47	34.586256209812305	36.0	36.0	36.0	32.0	36.0
48	34.558209672031566	36.0	36.0	36.0	32.0	36.0
49	34.53052689640743	36.0	36.0	36.0	32.0	36.0
50	34.11267116733178	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	4.0
20	9.0
21	21.0
22	56.0
23	167.0
24	373.0
25	957.0
26	1985.0
27	3830.0
28	6826.0
29	10581.0
30	16139.0
31	23583.0
32	35996.0
33	60942.0
34	139520.0
35	540217.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.2384531227703	17.72915378395091	12.130880464253437	25.90151262902535
2	16.119218807954578	19.6681788353448	37.68557353039078	26.527028826309834
3	19.112537104422575	22.73138478400679	28.500357224797224	29.655720886773413
4	12.746891676147069	15.898783774305791	34.877539351100616	36.47678519844652
5	14.681012685313638	36.548229988029135	33.0072550341235	15.76350229253372
6	35.197757507961775	34.79987684363064	16.152148044417128	13.850217603990455
7	30.992892372763485	29.954743708162894	19.474232919524162	19.578130999549458
8	28.17147700512001	33.762834206481386	18.493739367981085	19.571949420417518
9	26.894029902200284	14.345543140884132	18.273698926188377	40.48672803072721
10	16.86358562497548	26.624180197786757	30.310065631727667	26.20216854551009
11	37.788468739635455	20.941882457272808	21.47504365740262	19.794605145689122
12	24.69802391557865	23.282680047407958	28.491730354763206	23.52756568225019
13	29.93346481076641	19.049487107246833	24.85803171387848	26.159016368108283
14	23.652742659671972	19.49349091605059	24.757462176462685	32.09630424781475
15	25.498300660121327	26.84136760305703	21.974562801872068	25.685768934949575
16	25.970245065114923	25.649276930118102	23.516384234520718	24.864093770246253
17	24.196246117195606	25.883579467171653	24.954083943467083	24.966090472165657
18	24.96056262013987	24.986834393912556	26.032474765426343	24.02012822052123
19	26.023378256770908	24.637872395564003	24.746406659938256	24.59234268772683
20	25.979900333805674	24.365555249117936	24.73478616465844	24.91975825241795
21	27.072701314417703	24.099361751954625	24.19672162328268	24.63121531034499
22	26.020644096770244	24.1307451537014	25.021130301744275	24.82748044778408
23	24.878864680316877	24.103313330353494	25.154658538672958	25.863163450656675
24	25.023300047551118	24.669281978126488	25.118758915834523	25.188659058487872
25	25.285126354305604	24.178381989667216	25.234365898483844	25.302125757543337
26	24.455185662455243	25.471348210422207	25.705536350278884	24.367929776843667
27	25.151003260790745	24.380681568270354	25.37472940667398	25.09358576426492
28	24.771872429255488	24.80206753241813	25.353544239392484	25.072515798933896
29	24.537715628688915	24.573973226438802	25.316481277408663	25.57182986746362
30	24.81781934812018	25.022170761223872	25.300820971994703	24.859188918661243
31	25.221230521966138	24.61257911279547	24.775797547325023	25.39039281791337
32	25.18577516776529	24.681974072907316	24.727028488893907	25.405222270433487
33	24.970994094207377	24.344514079751974	25.17023138153703	25.514260444503616
34	25.297224556852775	24.716981356373463	25.321713411451984	24.664080675321774
35	25.650642469689387	24.402792653888994	25.43868512744188	24.50787974897974
36	24.526543407270744	25.23088847334842	24.886502370997864	25.356065748382978
37	25.499888253279885	24.939014821894748	24.85532366129807	24.7057732635273
38	24.998306009564804	24.605062475556018	25.21109498352966	25.185536531349523
39	25.67151013848058	24.18244866565779	24.87669415514247	25.269347040719165
40	26.007955226293618	24.61959467812955	24.83678234158496	24.535667753991877
41	24.594182379283	24.898032936164043	25.924539023279756	24.5832456612732
42	26.00685003976637	25.1409061026222	24.83989428921624	24.012349568395187
43	25.226736410378344	24.32193063274586	25.37367373019883	25.077659226676968
44	24.759471640062817	25.01768372348482	24.807143392112643	25.415701244339722
45	24.974470477886502	25.123546243801886	24.8673594211961	25.034623857115513
46	24.868370841749	24.89297886681828	24.542879186484846	25.69577110494788
47	25.0641046215955	24.79508513402829	25.224351315913047	24.916458928463165
48	25.784466289496745	24.90474656344622	24.170180592194033	25.14060655486301
49	25.15668465726717	25.379585819919637	24.550987897952876	24.912741624860317
50	25.32944047722358	25.47780014003821	24.19284854118942	24.99991084154879
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	439.0
1	245.0
2	51.0
3	127.0
4	203.0
5	180.0
6	157.0
7	122.0
8	87.0
9	126.5
10	166.0
11	285.5
12	405.0
13	775.0
14	1145.0
15	1450.5
16	1756.0
17	1803.0
18	1850.0
19	1851.5
20	1853.0
21	2006.0
22	2159.0
23	2582.5
24	3006.0
25	3915.5
26	4825.0
27	6723.0
28	8621.0
29	9964.0
30	11307.0
31	12415.5
32	13524.0
33	15769.5
34	18015.0
35	20799.0
36	23583.0
37	27119.0
38	30655.0
39	32990.5
40	35326.0
41	38121.0
42	40916.0
43	42148.5
44	43381.0
45	47560.5
46	51740.0
47	55005.0
48	58270.0
49	60892.0
50	63514.0
51	61670.0
52	59826.0
53	60219.5
54	60613.0
55	61318.0
56	62023.0
57	60254.5
58	58486.0
59	53792.0
60	49098.0
61	44129.5
62	39161.0
63	33855.0
64	28549.0
65	24234.0
66	19919.0
67	17477.5
68	15036.0
69	13455.5
70	11875.0
71	9305.0
72	6735.0
73	5988.0
74	5241.0
75	3882.0
76	2523.0
77	2241.5
78	1960.0
79	1706.5
80	1453.0
81	1116.5
82	780.0
83	648.5
84	517.0
85	395.0
86	273.0
87	188.5
88	104.0
89	69.5
90	35.0
91	25.5
92	16.0
93	13.0
94	10.0
95	11.5
96	13.0
97	9.0
98	5.0
99	4.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.034355314790973465
2	0.003922925218346451
3	2.377530435361486E-4
4	0.0
5	0.0
6	2.377530435361486E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.755060870722972E-4
17	0.0
18	2.377530435361486E-4
19	0.0
20	1.188765217680743E-4
21	0.0
22	0.0
23	1.188765217680743E-4
24	0.0010698886959126686
25	3.566295653042229E-4
26	5.943826088403714E-4
27	2.377530435361486E-4
28	0.0015453947829849658
29	7.132591306084458E-4
30	0.001307641739448817
31	5.943826088403714E-4
32	4.755060870722972E-4
33	1.188765217680743E-4
34	0.0011887652176807428
35	2.377530435361486E-4
36	2.377530435361486E-4
37	0.00249640695712956
38	0.0
39	9.510121741445944E-4
40	1.188765217680743E-4
41	7.132591306084458E-4
42	0.005468320001331417
43	0.0040418017401145255
44	0.004755060870722971
45	0.0030907895659699315
46	0.0023775304353614857
47	7.132591306084458E-4
48	0.0038040486965783774
49	0.0034474191312741545
50	0.0011887652176807428
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	841209.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.57745071068598
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.65892433738263	39.655388617149626
2	15.827479706205642	17.276469870796422
3	5.428353206411596	8.887970386893693
4	2.3647176291616727	5.162410394010457
5	1.1838479912561726	3.2305702695864182
6	0.649786872703201	2.127822661044585
7	0.4034063805823866	1.5411824296828005
8	0.28972817402524464	1.2650100109887872
9	0.1781177326733799	0.8749090598112482
>10	0.8709404929573776	8.466043920250137
>50	0.07552438552904335	2.844345478991116
>100	0.06370053645126947	6.681428711445871
>500	0.005253652473958299	1.864468060562833
>1k	2.189021864149291E-4	0.12198012878602155
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1021	0.12137292872520386	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	901	0.10710774611303493	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	894	0.1062756104606584	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	1.1887652176807428E-4	0.0
9	0.0	0.0	0.0	1.1887652176807428E-4	0.0
10	0.0	0.0	0.0	2.3775304353614856E-4	1.1887652176807428E-4
11	0.0	0.0	0.0	2.3775304353614856E-4	2.3775304353614856E-4
12	0.0	0.0	0.0	2.3775304353614856E-4	0.001307641739448817
13	0.0	0.0	0.0	2.3775304353614856E-4	0.0015453947829849658
14	0.0	0.0	0.0	2.3775304353614856E-4	0.0015453947829849658
15	0.0	0.0	0.0	2.3775304353614856E-4	0.00166427130475304
16	0.0	0.0	0.0	3.5662956530422283E-4	0.00166427130475304
17	0.0	0.0	0.0	3.5662956530422283E-4	0.0017831478265211143
18	0.0	0.0	0.0	7.132591306084457E-4	0.0017831478265211143
19	0.0	0.0	0.0	0.0010698886959126686	0.0017831478265211143
20	0.0	0.0	0.0	0.0011887652176807428	0.0017831478265211143
21	0.0	0.0	0.0	0.0011887652176807428	0.0017831478265211143
22	0.0	0.0	0.0	0.0011887652176807428	0.0017831478265211143
23	0.0	0.0	0.0	0.00166427130475304	0.0017831478265211143
24	0.0	0.0	0.0	0.0017831478265211143	0.0017831478265211143
25	0.0	0.0	0.0	0.002139777391825337	0.0017831478265211143
26	0.0	0.0	0.0	0.00249640695712956	0.002139777391825337
27	0.0	0.0	0.0	0.0027341600006657086	0.002258653913593411
28	0.0	0.0	0.0	0.0035662956530422285	0.002258653913593411
29	0.0	0.0	0.0	0.004398431305418749	0.002258653913593411
30	0.0	0.0	0.0	0.0072514678278525315	0.002258653913593411
31	0.0	0.0	0.0	0.01224428174211165	0.002258653913593411
32	0.0	0.0	0.0	0.019852379135268404	0.002258653913593411
33	0.0	0.0	0.0	0.023775304353614857	0.002258653913593411
34	0.0	0.0	0.0	0.029600253920250497	0.0023775304353614857
35	0.0	0.0	0.0	0.03542520348688614	0.0023775304353614857
36	0.0	0.0	0.0	0.043984313054187485	0.0023775304353614857
37	0.0	0.0	0.0	0.06003264349287751	0.0023775304353614857
38	0.0	0.0	0.0	0.07786412175808866	0.0023775304353614857
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	185	0.0	26.161173	4
AGGACGT	225	0.0	22.488037	5
GTATCAA	1370	0.0	22.325632	1
GTGTTAT	60	2.8678903E-4	22.0044	1
TATACTA	60	2.8725062E-4	21.999168	5
GTCCTAG	145	1.2732926E-11	21.245628	1
GGTATCA	690	0.0	21.047686	1
CGCAATA	305	0.0	20.918484	36
TCTATAG	65	4.9317075E-4	20.306923	3
ATACGAA	315	0.0	20.25561	40
CGAATGC	310	0.0	19.87258	43
GATATAC	370	0.0	19.625546	1
TACGAAT	315	0.0	19.55714	41
CTAGCGG	315	0.0	19.555979	29
TGTAGGA	305	0.0	19.474672	2
GGACGTG	250	0.0	19.359268	6
TAGCGGC	330	0.0	19.333752	30
GCGCAAT	330	0.0	19.333752	35
TATGGCG	190	1.8189894E-12	18.525616	16
GGACCGT	60	0.007411891	18.33264	6
>>END_MODULE