FastQCFastQC Report
Thu 2 Feb 2017
SRR4062071_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062071_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1877007
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68880.366967198310928No Hit
TATCAACGCAGAGTACTTTTTTTTT41620.22173598713270648No Hit
GTATATGGATGATAGTAATGAAGAC40320.21481006730395785No Hit
ATCTTATACCACACGCTAGGATTAC39640.21118727847045854No Hit
CCTCACAACCGTCCCAACGATCATC32190.17149643022109134No Hit
GGTATCAACGCAGAGTACTTTTTTT25760.13723976522197306No Hit
GTTGTGAGGAGTCCAGACAGGATCG22450.11960530781185152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTATA6050.015.5422672
TTATACC6100.015.4193974
CACGCTA5550.015.40759112
ACGCTAG5500.015.20012913
GGTATCA9500.015.0940851
ACGTTAG1101.2732926E-1114.6760671
ATCTTAT6050.014.5975851
CTTATAC6700.014.4635813
TATACCA6850.014.4254055
GTATCAA30050.013.2094671
ACACGCT6650.013.14542411
CGCTAGG6450.013.10828714
AACGATC4750.012.99872216
CCACACG6750.012.8099099
CCAACGA4750.012.79976614
ATACCAC7350.012.4105866
AGCCTAG3150.012.3602361
CACACGC6850.012.34547610
CGTCCCA4900.012.21667110
CAACGAT4900.012.213737515
Produced by FastQC (version 0.11.5)