##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062071_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1877007
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.221950157884333	32.0	32.0	32.0	32.0	32.0
2	30.746003078304984	32.0	32.0	32.0	32.0	32.0
3	30.784235754048865	32.0	32.0	32.0	32.0	32.0
4	30.776740843268033	32.0	32.0	32.0	32.0	32.0
5	30.685870111299533	32.0	32.0	32.0	32.0	32.0
6	34.34221449360604	36.0	36.0	36.0	32.0	36.0
7	34.28817953262828	36.0	36.0	36.0	32.0	36.0
8	34.236993255752374	36.0	36.0	36.0	32.0	36.0
9	34.390942068942735	36.0	36.0	36.0	32.0	36.0
10	34.05451018563063	36.0	36.0	36.0	32.0	36.0
11	34.36614940700807	36.0	36.0	36.0	32.0	36.0
12	34.2046039252917	36.0	36.0	36.0	32.0	36.0
13	34.278858310064905	36.0	36.0	36.0	32.0	36.0
14	34.187794185104266	36.0	36.0	36.0	32.0	36.0
15	34.14887104842976	36.0	36.0	36.0	32.0	36.0
16	34.1403947880855	36.0	36.0	36.0	32.0	36.0
17	34.07834121023523	36.0	36.0	36.0	32.0	36.0
18	34.09175352036513	36.0	36.0	36.0	32.0	36.0
19	34.092381115254234	36.0	36.0	36.0	32.0	36.0
20	34.066278388945804	36.0	36.0	36.0	32.0	36.0
21	34.06442437348396	36.0	36.0	36.0	32.0	36.0
22	34.0353680087501	36.0	36.0	36.0	32.0	36.0
23	34.010496497882	36.0	36.0	36.0	32.0	36.0
24	33.964752395702305	36.0	36.0	36.0	32.0	36.0
25	33.612456959403985	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	25.0
5	164.0
6	397.0
7	69.0
8	327.0
9	247.0
10	132.0
11	40.0
12	82.0
13	90.0
14	314.0
15	548.0
16	789.0
17	1115.0
18	1551.0
19	2208.0
20	3114.0
21	4307.0
22	6030.0
23	8533.0
24	11932.0
25	16305.0
26	21986.0
27	28158.0
28	36910.0
29	48782.0
30	64041.0
31	87505.0
32	124021.0
33	178323.0
34	391826.0
35	837136.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.104616210813674	18.115827754377477	12.460455387710349	25.319100647098498
2	16.969214253558196	18.944115758881836	37.75983548259062	26.326834504969355
3	19.438357587526873	22.779495949642914	27.96027693201101	29.821869530819207
4	13.750472213090253	15.316061745491396	34.30438283014555	36.6290832112728
5	15.143517694422268	36.04394479932891	32.70038596128449	16.11215154496433
6	33.676937839855	34.74885382236913	16.34390659985073	15.230301737925153
7	30.489814008385167	30.35381234009804	19.05335372113898	20.103019930377812
8	27.65672160018518	33.560239219214765	18.062778776628285	20.720260403971768
9	26.84149353867063	15.170882386043294	18.364449166804018	39.623174908482056
10	16.936828319093944	26.286013032843513	29.45102547619492	27.32613317186762
11	36.70968468216127	22.186369429945938	21.27825874938845	19.825687138504353
12	23.69235447413837	23.883072507528695	27.73125561817983	24.69331740015311
13	29.33017741125836	20.02815577537474	24.568501273091282	26.073165540275618
14	22.943730794525898	19.219155628547398	24.299881960175828	33.53723161675088
15	26.28793023701213	26.780605197974243	21.10083912121938	25.83062544379424
16	25.31244365879392	26.981476878369136	22.509865378522225	25.196214084314718
17	23.79835967193439	26.732493165299726	24.250023338000933	25.219123824764957
18	23.93009723237097	26.222711732421633	24.97623860599822	24.870952429209183
19	26.039579619199888	25.05320887797662	24.951190753627564	23.956020749195932
20	26.180431906496516	24.5226413092109	24.61260183216577	24.68432495212682
21	25.9487869168218	24.304857505320587	25.11215760136045	24.634197976497163
22	25.72134217135153	24.23201984340943	24.642988756219065	25.403649229019976
23	24.94612051728569	24.59442426000356	25.045988177972383	25.413467044738365
24	25.081414291694333	24.851849185590456	24.48374513740578	25.582991385309434
25	24.945263317304256	24.045427447366936	24.725516732414672	26.28379250291413
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	248.0
1	248.0
2	495.0
3	742.0
4	742.0
5	742.0
6	1541.0
7	2340.0
8	2340.0
9	2340.0
10	2688.0
11	3036.0
12	3036.0
13	3036.0
14	3520.5
15	4005.0
16	4005.0
17	4005.0
18	6993.5
19	9982.0
20	9982.0
21	9982.0
22	17100.5
23	24219.0
24	24219.0
25	24219.0
26	34953.5
27	45688.0
28	45688.0
29	45688.0
30	62482.0
31	79276.0
32	79276.0
33	79276.0
34	94851.5
35	110427.0
36	110427.0
37	110427.0
38	131579.0
39	152731.0
40	152731.0
41	152731.0
42	168983.0
43	185235.0
44	185235.0
45	185235.0
46	206687.0
47	228139.0
48	228139.0
49	228139.0
50	240619.0
51	253099.0
52	253099.0
53	253099.0
54	246912.5
55	240726.0
56	240726.0
57	240726.0
58	218095.0
59	195464.0
60	195464.0
61	195464.0
62	171040.0
63	146616.0
64	146616.0
65	146616.0
66	122509.0
67	98402.0
68	98402.0
69	98402.0
70	76560.0
71	54718.0
72	54718.0
73	54718.0
74	40033.5
75	25349.0
76	25349.0
77	25349.0
78	17851.5
79	10354.0
80	10354.0
81	10354.0
82	6949.0
83	3544.0
84	3544.0
85	3544.0
86	2508.5
87	1473.0
88	1473.0
89	1473.0
90	887.5
91	302.0
92	302.0
93	302.0
94	239.0
95	176.0
96	176.0
97	176.0
98	446.0
99	716.0
100	716.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	6.925919828748641E-4
2	1.065526127499791E-4
3	6.393156764998746E-4
4	0.010974919113247847
5	0.03478942806286817
6	0.06430450179461239
7	0.10815090194122878
8	0.11049505942172831
9	0.11923237366722661
10	0.13383008161397372
11	0.14256739585947203
12	0.13383008161397372
13	0.12978108232947452
14	0.11806029492697682
15	0.13564147603072338
16	0.12088393916485128
17	0.1269041617852251
18	0.11278594059585287
19	0.09856116679373066
20	0.0927007730924818
21	0.09291387831798177
22	0.10261016607822987
23	0.08124636722185904
24	0.091741799577732
25	0.08870505011435759
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1877007.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.9701436586375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.41988788571506	34.484720261886125
2	15.79744397332114	15.472062016256267
3	6.0205471598288485	8.844811779612622
4	2.8199947482372805	5.523821917511316
5	1.477562945156734	3.6178234844502386
6	0.8731496782472308	2.5654959107556
7	0.5448023476478184	1.8675334460913642
8	0.39759349824829054	1.557616858156722
9	0.2597951393335872	1.1449984765483359
>10	1.2151166929615569	10.59415083907773
>50	0.09641007001229816	3.2929638038491187
>100	0.07016719497423579	6.751170037053465
>500	0.005128221983392374	1.7582823937383438
>1k	0.0022913332266221247	2.1565100863427102
>5k	1.0911110602962497E-4	0.36803868867018297
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6888	0.366967198310928	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4162	0.22173598713270648	No Hit
GTATATGGATGATAGTAATGAAGAC	4032	0.21481006730395785	No Hit
ATCTTATACCACACGCTAGGATTAC	3964	0.21118727847045854	No Hit
CCTCACAACCGTCCCAACGATCATC	3219	0.17149643022109134	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2576	0.13723976522197306	No Hit
GTTGTGAGGAGTCCAGACAGGATCG	2245	0.11960530781185152	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.327630637498954E-5	0.0	0.0	0.0	5.327630637498954E-5
2	5.327630637498954E-5	0.0	0.0	0.0	5.327630637498954E-5
3	5.327630637498954E-5	0.0	0.0	0.0	5.327630637498954E-5
4	5.327630637498954E-5	0.0	0.0	0.0	5.327630637498954E-5
5	1.0655261274997909E-4	0.0	0.0	0.0	5.327630637498954E-5
6	1.0655261274997909E-4	0.0	0.0	0.0	1.5982891912496864E-4
7	1.0655261274997909E-4	0.0	0.0	0.0	1.5982891912496864E-4
8	1.0655261274997909E-4	0.0	0.0	0.0	1.5982891912496864E-4
9	1.0655261274997909E-4	0.0	0.0	0.0	1.5982891912496864E-4
10	1.0655261274997909E-4	0.0	0.0	0.0	1.5982891912496864E-4
11	1.0655261274997909E-4	0.0	0.0	5.327630637498954E-5	1.5982891912496864E-4
12	1.0655261274997909E-4	0.0	1.0655261274997909E-4	5.327630637498954E-5	2.1310522549995817E-4
13	1.0655261274997909E-4	0.0	1.0655261274997909E-4	5.327630637498954E-5	3.196578382499373E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTATA	605	0.0	15.542267	2
TTATACC	610	0.0	15.419397	4
CACGCTA	555	0.0	15.407591	12
ACGCTAG	550	0.0	15.200129	13
GGTATCA	950	0.0	15.094085	1
ACGTTAG	110	1.2732926E-11	14.676067	1
ATCTTAT	605	0.0	14.597585	1
CTTATAC	670	0.0	14.463581	3
TATACCA	685	0.0	14.425405	5
GTATCAA	3005	0.0	13.209467	1
ACACGCT	665	0.0	13.145424	11
CGCTAGG	645	0.0	13.108287	14
AACGATC	475	0.0	12.998722	16
CCACACG	675	0.0	12.809909	9
CCAACGA	475	0.0	12.799766	14
ATACCAC	735	0.0	12.410586	6
AGCCTAG	315	0.0	12.360236	1
CACACGC	685	0.0	12.345476	10
CGTCCCA	490	0.0	12.216671	10
CAACGAT	490	0.0	12.2137375	15
>>END_MODULE