FastQCFastQC Report
Thu 2 Feb 2017
SRR4062071_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062071_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1877007
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTTATACCACACGCTAGGATTACATATATTTCAGAAACAGCACATGAA39670.2113471073895835No Hit
GTATATGGATGATAGTAATGAAGACCAGAGGCTTCCTCATCACCTTGAAC39640.21118727847045854No Hit
CCTCACAACCGTCCCAACGATCATCTTCCCGCGAGGAAGGAGTATCTAAA32120.1711234960764664No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24660.1313793715207242No Hit
GTTGTGAGGAGTCCAGACAGGATCGCCAACAAAGAGTCGCATAGCCTTGA21340.1136916378042277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12450.031.6483441
TTATACC6750.027.0505084
GTATCAA34450.026.7726961
ATCTTAT6950.025.9714581
CACGCTA6500.025.38403112
ACGCTAG6800.024.26414713
GTATATG9400.023.885791
TATACCA8850.023.1174965
GATAGTA9500.022.23106811
CGCTAGG7550.021.85380414
TATATGG10350.021.469252
ACACGCT7650.021.28055611
GTATCTA7850.021.02086442
GTATTAG1700.020.7175461
TATCTAA7900.020.60986743
CTTATAC9800.020.4275593
TGATAGT10250.020.38977610
TATGGAT10600.020.3386144
AGGATTA8000.020.07400318
ATGATAG10600.019.923549
Produced by FastQC (version 0.11.5)