##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062071_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1877007
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.306684524884563	32.0	32.0	32.0	32.0	32.0
2	31.504990657999677	32.0	32.0	32.0	32.0	32.0
3	31.60196632191569	32.0	32.0	32.0	32.0	32.0
4	31.66748712178484	32.0	32.0	32.0	32.0	32.0
5	31.64157832123162	32.0	32.0	32.0	32.0	32.0
6	35.28921842060259	36.0	36.0	36.0	36.0	36.0
7	35.29591045744635	36.0	36.0	36.0	36.0	36.0
8	35.24861761304034	36.0	36.0	36.0	36.0	36.0
9	35.3292054851154	36.0	36.0	36.0	36.0	36.0
10	35.22543655937351	36.0	36.0	36.0	36.0	36.0
11	35.32841113538735	36.0	36.0	36.0	36.0	36.0
12	35.26832558429457	36.0	36.0	36.0	36.0	36.0
13	35.29002875322255	36.0	36.0	36.0	36.0	36.0
14	35.25393618670575	36.0	36.0	36.0	36.0	36.0
15	35.23537259051245	36.0	36.0	36.0	36.0	36.0
16	35.249875466633846	36.0	36.0	36.0	36.0	36.0
17	35.23419678243075	36.0	36.0	36.0	36.0	36.0
18	35.22810996442741	36.0	36.0	36.0	36.0	36.0
19	35.22362303390451	36.0	36.0	36.0	36.0	36.0
20	35.215054072787154	36.0	36.0	36.0	36.0	36.0
21	35.21176958849914	36.0	36.0	36.0	36.0	36.0
22	35.18973504094551	36.0	36.0	36.0	36.0	36.0
23	35.162664816913306	36.0	36.0	36.0	36.0	36.0
24	35.14511506883032	36.0	36.0	36.0	36.0	36.0
25	35.129113530210596	36.0	36.0	36.0	36.0	36.0
26	35.092310257766755	36.0	36.0	36.0	36.0	36.0
27	35.08536622399384	36.0	36.0	36.0	36.0	36.0
28	35.06713773576764	36.0	36.0	36.0	36.0	36.0
29	35.048558689445485	36.0	36.0	36.0	36.0	36.0
30	35.02197754190581	36.0	36.0	36.0	36.0	36.0
31	35.015466644503725	36.0	36.0	36.0	36.0	36.0
32	34.99798189351451	36.0	36.0	36.0	36.0	36.0
33	34.96397882373374	36.0	36.0	36.0	36.0	36.0
34	34.966895168744706	36.0	36.0	36.0	36.0	36.0
35	34.939349187296585	36.0	36.0	36.0	36.0	36.0
36	34.919498435541264	36.0	36.0	36.0	36.0	36.0
37	34.90740524675721	36.0	36.0	36.0	36.0	36.0
38	34.89511014077198	36.0	36.0	36.0	32.0	36.0
39	34.86815126422011	36.0	36.0	36.0	32.0	36.0
40	34.85879594482066	36.0	36.0	36.0	32.0	36.0
41	34.824846151346264	36.0	36.0	36.0	32.0	36.0
42	34.80196983815191	36.0	36.0	36.0	32.0	36.0
43	34.783523982595696	36.0	36.0	36.0	32.0	36.0
44	34.75518045484114	36.0	36.0	36.0	32.0	36.0
45	34.719528483378056	36.0	36.0	36.0	32.0	36.0
46	34.69392975092794	36.0	36.0	36.0	32.0	36.0
47	34.65175729232762	36.0	36.0	36.0	32.0	36.0
48	34.615795785524504	36.0	36.0	36.0	32.0	36.0
49	34.596285469366926	36.0	36.0	36.0	32.0	36.0
50	34.1984680930865	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	6.0
20	18.0
21	47.0
22	138.0
23	330.0
24	953.0
25	2112.0
26	4520.0
27	8237.0
28	14294.0
29	22750.0
30	33922.0
31	50205.0
32	75702.0
33	126576.0
34	290271.0
35	1246923.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.65732290447532	18.61722765480736	12.905978259710968	25.81947118100635
2	16.48206389960536	19.30851350062576	37.512980090179994	26.696442509588884
3	18.946330371517984	23.09805743414232	28.179618348835007	29.775993845504694
4	13.537083239433844	15.56621792033807	34.47302007930711	36.42367876092097
5	14.806034080889502	36.319881939579275	32.60540062493174	16.26868335459948
6	33.76750063798512	34.904525991700595	16.230874431207624	15.097098939106651
7	30.52711044764351	30.309743117633552	19.098916519757253	20.064229914965686
8	27.70831435364919	33.54377474351454	18.097055578375574	20.6508553244607
9	26.92227572939259	14.948372595307315	18.300571068728033	39.82878060657205
10	16.901384438835038	26.170347883810706	29.614982583966164	27.31328509338809
11	37.00130047463862	22.06512815349117	21.25863142758658	19.67493994428364
12	23.64935239985786	23.810300121416702	27.75636958199943	24.78397789672601
13	29.4173117095461	19.88596739383497	24.687068295429906	26.00965260118902
14	22.973222795652866	18.97046734508715	24.40427766119146	33.65203219806852
15	26.4964382125373	26.672569681413016	21.034604559279746	25.79638754676994
16	25.329823456610562	26.87208076339286	22.579780701964687	25.21831507803189
17	23.811845134301578	26.613432981336775	24.393728952529212	25.180992931832435
18	24.078026388834132	26.121653313841296	25.010415523445317	24.78990477387925
19	26.224011124104624	24.793807155548333	25.058324298550083	23.92385742179696
20	26.160999124136126	24.408685330452144	24.825839476101276	24.604476069310454
21	26.01647196840502	24.109872792163266	25.291754372786034	24.581900866645675
22	25.701236063584204	24.14583429896639	24.814238838747006	25.3386907987024
23	24.98565533922392	24.328384847136793	25.290342860035004	25.39561695360428
24	25.163586031708146	24.6663015438515	24.583456313658967	25.586656110781387
25	25.02041818793044	23.736669573783313	24.99639052255111	26.24652171573514
26	25.10162504168891	24.475625440197593	25.027890280117504	25.39485923799599
27	25.140929750095765	24.688558704613055	24.978702705755303	25.191808839535877
28	25.007898330242373	24.5098107117874	24.676994255130186	25.805296702840042
29	25.174240995461904	24.951225307645505	24.874187399240704	25.000346297651888
30	25.31261571083413	24.696468005871118	24.308611949482813	25.68230433381194
31	25.156633258675033	24.558336831831287	24.866009304228673	25.419020605265008
32	25.083351536236208	24.222611734745524	24.780100053809555	25.91393667520871
33	24.78797316786388	24.62654561553711	24.908004941920712	25.677476274678302
34	25.187414923401093	24.078029392882737	25.102331664877447	25.632224018838723
35	25.15010639306724	24.205248582580094	24.87338852063024	25.771256503722423
36	25.358736964585017	24.147976400664465	24.66539726649199	25.827889368258532
37	25.455001702210634	24.344169409117136	24.919085057787253	25.281743830884974
38	24.892994481637245	24.202266801491312	24.71696947159466	26.187769245276787
39	25.235496600676292	24.901664419275317	24.74439142222845	25.118447557819934
40	25.43291040780392	24.441170907909143	24.94921430683456	25.17670437745238
41	25.126172956978394	25.264373168274474	24.58706426842594	25.022389606321195
42	25.430641549483752	24.790493305748974	24.63774216011538	25.141122984651894
43	25.169385555462615	24.1451070923758	24.697444924720195	25.988062427441395
44	24.86758955719689	24.50502922593236	24.793479135332237	25.83390208153851
45	25.292014747250875	24.3663157446083	24.94240687068451	25.39926263745631
46	25.164628259799375	24.621568113477355	24.441436027269685	25.77236759945358
47	25.277505622036145	24.497639570610165	24.879847457816748	25.345007349536946
48	25.17761764588356	25.100683298480224	23.89280338852112	25.82889566711509
49	25.561034345888377	25.059045731471784	24.054695554188076	25.32522436845176
50	24.949599889183688	25.723129708361302	23.755873797270084	25.571396605184926
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	104.0
1	90.5
2	77.0
3	210.0
4	343.0
5	377.5
6	412.0
7	354.0
8	296.0
9	406.0
10	516.0
11	905.5
12	1295.0
13	2568.0
14	3841.0
15	5076.5
16	6312.0
17	5794.5
18	5277.0
19	4650.0
20	4023.0
21	4056.5
22	4090.0
23	4931.5
24	5773.0
25	7164.5
26	8556.0
27	10680.0
28	12804.0
29	15706.0
30	18608.0
31	23143.5
32	27679.0
33	35034.0
34	42389.0
35	47086.5
36	51784.0
37	57311.5
38	62839.0
39	68867.5
40	74896.0
41	85836.5
42	96777.0
43	102619.5
44	108462.0
45	108476.0
46	108490.0
47	115216.0
48	121942.0
49	126290.0
50	130638.0
51	137055.5
52	143473.0
53	140279.0
54	137085.0
55	136614.5
56	136144.0
57	132551.5
58	128959.0
59	120012.0
60	111065.0
61	102339.5
62	93614.0
63	83242.0
64	72870.0
65	62891.0
66	52912.0
67	44820.5
68	36729.0
69	31942.0
70	27155.0
71	23199.5
72	19244.0
73	14054.0
74	8864.0
75	6597.5
76	4331.0
77	3543.0
78	2755.0
79	2336.0
80	1917.0
81	1426.5
82	936.0
83	659.0
84	382.0
85	273.0
86	164.0
87	111.5
88	59.0
89	46.0
90	33.0
91	29.0
92	25.0
93	23.5
94	22.0
95	20.0
96	18.0
97	19.0
98	20.0
99	14.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03596150680311794
2	0.005007972799249017
3	2.663815318749477E-4
4	0.0
5	1.065526127499791E-4
6	2.131052254999582E-4
7	0.0
8	0.0
9	0.0
10	5.327630637498955E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	9.589735147498118E-4
17	0.0
18	5.327630637498955E-5
19	1.065526127499791E-4
20	1.5982891912496864E-4
21	0.0
22	0.0
23	1.065526127499791E-4
24	6.925919828748641E-4
25	2.131052254999582E-4
26	4.794867573749059E-4
27	4.262104509999164E-4
28	0.0014917365784997071
29	4.794867573749059E-4
30	0.00117207874024977
31	5.86039370124885E-4
32	9.056972083748223E-4
33	2.131052254999582E-4
34	0.00117207874024977
35	2.663815318749477E-4
36	2.663815318749477E-4
37	0.0019179470294996236
38	5.327630637498955E-5
39	8.524209019998328E-4
40	1.065526127499791E-4
41	0.001065526127499791
42	0.005221078024748976
43	0.004048999284499206
44	0.004155551897249184
45	0.0025039863996245085
46	0.002717091625124467
47	9.589735147498118E-4
48	0.004368657122749142
49	0.003835894058999247
50	0.0014384602721247176
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1877007.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.424404399966186
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.50147768963521	35.04570617417393
2	16.473753441779703	16.61358411067269
3	6.273266183736835	9.489771328721362
4	2.870345017783516	5.789417517765765
5	1.5194758330782325	3.8309331941556146
6	0.878411668537771	2.657603112239857
7	0.5656162703277964	1.996460448514658
8	0.3609764134139749	1.456161651906852
9	0.25896485712014966	1.17523338127253
>10	1.1470803327071433	10.172434512391556
>50	0.08554550512332075	2.97097208678068
>100	0.05955734712794266	5.774180351984239
>500	0.0034027320790740037	1.163371025468121
>1k	0.0021267075494212527	1.864171103952167
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATCTTATACCACACGCTAGGATTACATATATTTCAGAAACAGCACATGAA	3967	0.2113471073895835	No Hit
GTATATGGATGATAGTAATGAAGACCAGAGGCTTCCTCATCACCTTGAAC	3964	0.21118727847045854	No Hit
CCTCACAACCGTCCCAACGATCATCTTCCCGCGAGGAAGGAGTATCTAAA	3212	0.1711234960764664	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2466	0.1313793715207242	No Hit
GTTGTGAGGAGTCCAGACAGGATCGCCAACAAAGAGTCGCATAGCCTTGA	2134	0.1136916378042277	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0655261274997909E-4
2	0.0	0.0	0.0	0.0	1.0655261274997909E-4
3	0.0	0.0	0.0	0.0	1.0655261274997909E-4
4	0.0	0.0	0.0	0.0	1.0655261274997909E-4
5	0.0	0.0	0.0	0.0	1.5982891912496864E-4
6	5.327630637498954E-5	0.0	0.0	0.0	2.1310522549995817E-4
7	5.327630637498954E-5	0.0	0.0	0.0	2.663815318749477E-4
8	5.327630637498954E-5	0.0	0.0	0.0	3.196578382499373E-4
9	5.327630637498954E-5	0.0	0.0	0.0	3.196578382499373E-4
10	5.327630637498954E-5	0.0	0.0	0.0	3.196578382499373E-4
11	5.327630637498954E-5	0.0	0.0	0.0	3.196578382499373E-4
12	5.327630637498954E-5	0.0	0.0	0.0	4.2621045099991634E-4
13	5.327630637498954E-5	0.0	0.0	0.0	4.794867573749059E-4
14	5.327630637498954E-5	0.0	0.0	0.0	4.794867573749059E-4
15	5.327630637498954E-5	0.0	0.0	0.0	4.794867573749059E-4
16	5.327630637498954E-5	0.0	0.0	5.327630637498954E-5	4.794867573749059E-4
17	5.327630637498954E-5	0.0	0.0	5.327630637498954E-5	4.794867573749059E-4
18	5.327630637498954E-5	0.0	0.0	5.327630637498954E-5	4.794867573749059E-4
19	5.327630637498954E-5	0.0	0.0	5.327630637498954E-5	5.327630637498955E-4
20	5.327630637498954E-5	0.0	0.0	1.0655261274997909E-4	6.393156764998746E-4
21	5.327630637498954E-5	0.0	0.0	2.1310522549995817E-4	6.393156764998746E-4
22	5.327630637498954E-5	0.0	0.0	4.2621045099991634E-4	6.393156764998746E-4
23	5.327630637498954E-5	0.0	0.0	4.794867573749059E-4	6.393156764998746E-4
24	1.5982891912496864E-4	0.0	0.0	7.458682892498536E-4	6.925919828748641E-4
25	1.5982891912496864E-4	0.0	0.0	0.0015450128848746969	6.925919828748641E-4
26	1.5982891912496864E-4	0.0	0.0	0.0018113944167496446	6.925919828748641E-4
27	1.5982891912496864E-4	0.0	5.327630637498954E-5	0.002131052254999582	6.925919828748641E-4
28	1.5982891912496864E-4	0.0	5.327630637498954E-5	0.002930196850624425	6.925919828748641E-4
29	1.5982891912496864E-4	0.0	5.327630637498954E-5	0.004262104509999164	7.458682892498536E-4
30	1.5982891912496864E-4	0.0	5.327630637498954E-5	0.006286604152248766	7.458682892498536E-4
31	2.1310522549995817E-4	0.0	5.327630637498954E-5	0.010388879743122961	7.991445956248432E-4
32	2.1310522549995817E-4	0.0	5.327630637498954E-5	0.015503405155121958	9.056972083748223E-4
33	2.1310522549995817E-4	0.0	5.327630637498954E-5	0.019605680745996154	9.589735147498118E-4
34	2.1310522549995817E-4	0.0	5.327630637498954E-5	0.02578573228549494	0.0010122498211248014
35	2.1310522549995817E-4	0.0	5.327630637498954E-5	0.03265837580786859	0.0010122498211248014
36	2.1310522549995817E-4	0.0	5.327630637498954E-5	0.0417686241979918	0.0010122498211248014
37	2.1310522549995817E-4	0.0	5.327630637498954E-5	0.05684581890211384	0.0010122498211248014
38	2.1310522549995817E-4	0.0	1.5982891912496864E-4	0.07613184180986006	0.0010122498211248014
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1245	0.0	31.648344	1
TTATACC	675	0.0	27.050508	4
GTATCAA	3445	0.0	26.772696	1
ATCTTAT	695	0.0	25.971458	1
CACGCTA	650	0.0	25.384031	12
ACGCTAG	680	0.0	24.264147	13
GTATATG	940	0.0	23.88579	1
TATACCA	885	0.0	23.117496	5
GATAGTA	950	0.0	22.231068	11
CGCTAGG	755	0.0	21.853804	14
TATATGG	1035	0.0	21.46925	2
ACACGCT	765	0.0	21.280556	11
GTATCTA	785	0.0	21.020864	42
GTATTAG	170	0.0	20.717546	1
TATCTAA	790	0.0	20.609867	43
CTTATAC	980	0.0	20.427559	3
TGATAGT	1025	0.0	20.389776	10
TATGGAT	1060	0.0	20.338614	4
AGGATTA	800	0.0	20.074003	18
ATGATAG	1060	0.0	19.92354	9
>>END_MODULE