FastQCFastQC Report
Thu 2 Feb 2017
SRR4062070_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062070_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800245
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31040.17242097603381762No Hit
GTCCTACAGTGGACATTTCTAAATT20970.11648414521356816No Hit
TATCAACGCAGAGTACTTTTTTTTT19660.10920735788739866No Hit
CTGTAGGACGTGGAATATGGCAAGA19360.107540918041711No Hit
GTCCTAAAGTGTGTATTTCTCATTT19290.1071520820777172No Hit
GTCCTACAGTGTGCATTTCTCATTT19170.10648550613944215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA1350.014.7786449
GGTATCA7300.014.0485841
CGCGTAA1450.013.1045810
GCGTAAC1550.012.87172111
TAACGTG604.0905582E-412.6674085
GCGATCG751.4779982E-512.66740814
GTATCAA20150.012.2997721
GCGCACT550.003067420812.09161811
GTCCTAC11750.011.7990391
TGTAGGA15150.011.7855352
AACCGCG658.0186815E-411.6933187
CGCACTC658.020553E-411.69299212
TAGAAAT3150.011.4597144
GTAGGAC14350.011.4514033
CTGTAGG15200.011.4324461
GGACGTG10500.011.4013016
ACTCGTG752.0730321E-411.40066715
TGAGTTA5300.011.29311512
AGGACGT11200.011.1970845
TAAGCTG1201.2744385E-711.0839825
Produced by FastQC (version 0.11.5)