##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062070_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1800245
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.303618674124913	32.0	32.0	32.0	32.0	32.0
2	30.868832297826128	32.0	32.0	32.0	32.0	32.0
3	30.90024913275693	32.0	32.0	32.0	32.0	32.0
4	30.90718985471422	32.0	32.0	32.0	32.0	32.0
5	30.83424978266847	32.0	32.0	32.0	32.0	32.0
6	34.50442189813053	36.0	36.0	36.0	32.0	36.0
7	34.44296859594111	36.0	36.0	36.0	32.0	36.0
8	34.407449541590175	36.0	36.0	36.0	32.0	36.0
9	34.52277939947063	36.0	36.0	36.0	32.0	36.0
10	34.2270118789387	36.0	36.0	36.0	32.0	36.0
11	34.5062916436374	36.0	36.0	36.0	32.0	36.0
12	34.33514382764568	36.0	36.0	36.0	32.0	36.0
13	34.420416665509414	36.0	36.0	36.0	32.0	36.0
14	34.334006204711024	36.0	36.0	36.0	32.0	36.0
15	34.28475457507172	36.0	36.0	36.0	32.0	36.0
16	34.284670697599495	36.0	36.0	36.0	32.0	36.0
17	34.22680912875747	36.0	36.0	36.0	32.0	36.0
18	34.232191173979096	36.0	36.0	36.0	32.0	36.0
19	34.2201328152557	36.0	36.0	36.0	32.0	36.0
20	34.20142202866832	36.0	36.0	36.0	32.0	36.0
21	34.181052023474585	36.0	36.0	36.0	32.0	36.0
22	34.14393985263117	36.0	36.0	36.0	32.0	36.0
23	34.11649969865213	36.0	36.0	36.0	32.0	36.0
24	34.08245822096437	36.0	36.0	36.0	32.0	36.0
25	33.742862221530956	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	30.0
5	123.0
6	350.0
7	84.0
8	315.0
9	244.0
10	124.0
11	24.0
12	64.0
13	72.0
14	264.0
15	385.0
16	615.0
17	860.0
18	1201.0
19	1571.0
20	2324.0
21	3163.0
22	4698.0
23	7057.0
24	9818.0
25	13972.0
26	19243.0
27	24737.0
28	32976.0
29	43951.0
30	57961.0
31	79197.0
32	113697.0
33	163595.0
34	366946.0
35	850583.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.274113262634685	17.19075914483928	11.790761477883574	25.744366114642464
2	16.264164778029595	18.90720126205395	38.75438830378172	26.074245656134735
3	19.79907078849662	22.308686194476724	28.042101146738542	29.850141870288116
4	12.939322948928126	15.087571545789027	34.97945358821958	36.99365191706327
5	14.940315085672564	36.306975436475284	33.42444105993162	15.328268417920532
6	35.302755761085855	34.66172726969552	16.201185010616186	13.834331958602444
7	31.00415920641452	29.815838346993473	19.665595355923532	19.514407090668477
8	27.788828061356412	34.49315898784716	18.601213893885767	19.11679905691066
9	26.965606679575888	14.514419435457052	18.52309923601294	39.99687464895412
10	16.649665728556016	26.609564279119436	30.941800060068747	25.798969932255805
11	37.39592881998522	20.857455301122254	22.34871576942588	19.39790010946664
12	24.962498685994003	23.027948142788095	28.88254705253855	23.127006118679354
13	30.001345912985055	19.573912869375253	24.974360913797053	25.450380303842635
14	23.081282059267103	20.151849149609173	24.587706146926536	32.17916264419719
15	24.83397592344983	28.557420414120223	21.53290558263605	25.075698079793895
16	25.18307684778595	25.852875618968806	24.634902944801105	24.329144588444134
17	23.610470011556476	26.614653255667285	25.49698964366235	24.27788708911389
18	24.360080097380262	25.113231248654987	27.049548159356195	23.477140494608552
19	25.873310548780793	24.70845169013231	24.873192122482816	24.545045638604083
20	25.812370007177577	24.9051375554094	24.97758049478027	24.304911942632753
21	25.993077037692675	24.360854684508176	24.368249111000907	25.27781916679825
22	25.903748108763935	25.00802089826276	24.807957175909838	24.280273817063467
23	24.203915490514806	25.07899693351805	25.654712798059155	25.062374777907987
24	25.087369918061864	24.870710312152173	25.598691035729654	24.443228734056312
25	24.730446307750984	25.0172067377026	25.64393227605945	24.608414678486966
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	515.0
1	515.0
2	533.0
3	551.0
4	551.0
5	551.0
6	1150.5
7	1750.0
8	1750.0
9	1750.0
10	2122.0
11	2494.0
12	2494.0
13	2494.0
14	3295.0
15	4096.0
16	4096.0
17	4096.0
18	7033.0
19	9970.0
20	9970.0
21	9970.0
22	16492.0
23	23014.0
24	23014.0
25	23014.0
26	36487.5
27	49961.0
28	49961.0
29	49961.0
30	66613.5
31	83266.0
32	83266.0
33	83266.0
34	99318.5
35	115371.0
36	115371.0
37	115371.0
38	132748.0
39	150125.0
40	150125.0
41	150125.0
42	170103.5
43	190082.0
44	190082.0
45	190082.0
46	210280.0
47	230478.0
48	230478.0
49	230478.0
50	236869.5
51	243261.0
52	243261.0
53	243261.0
54	226488.0
55	209715.0
56	209715.0
57	209715.0
58	193615.0
59	177515.0
60	177515.0
61	177515.0
62	156175.5
63	134836.0
64	134836.0
65	134836.0
66	109525.5
67	84215.0
68	84215.0
69	84215.0
70	64327.0
71	44439.0
72	44439.0
73	44439.0
74	33405.0
75	22371.0
76	22371.0
77	22371.0
78	17638.5
79	12906.0
80	12906.0
81	12906.0
82	9039.5
83	5173.0
84	5173.0
85	5173.0
86	3763.5
87	2354.0
88	2354.0
89	2354.0
90	1583.5
91	813.0
92	813.0
93	813.0
94	508.0
95	203.0
96	203.0
97	203.0
98	487.0
99	771.0
100	771.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001222055886837625
2	2.777399742812784E-4
3	4.99931953706301E-4
4	0.009665351104988487
5	0.030218109201803088
6	0.06138053431616253
7	0.1013750906126666
8	0.1055411902268858
9	0.11370674547075538
10	0.12803812814366933
11	0.13537046346469508
12	0.1282047721282381
13	0.12270552063746878
14	0.11181811364564266
15	0.12848251210251938
16	0.11626195323414312
17	0.11770620110040576
18	0.10742982205199848
19	0.09209857547167191
20	0.08821021583173401
21	0.0884879558060153
22	0.10098625464867281
23	0.08004466058786443
24	0.08726589991917766
25	0.08504398012492745
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1800245.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.94836110390486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.58182770212059	39.783004394667856
2	13.53759979656618	14.065122454243378
3	4.1940838761612715	6.536273510966723
4	1.7675476280277467	3.672848097965436
5	0.966827441086542	2.511255051736399
6	0.6080193488453481	1.8951365215187546
7	0.43001360080548673	1.5636951269963668
8	0.2866468435034223	1.1912666988488227
9	0.2120722865657202	0.9915126950382143
>10	1.2363788224752545	11.659411539937173
>50	0.09451163507909398	3.357090591520672
>100	0.07364487787250738	7.718017347229586
>500	0.00793202401905436	2.80671147821418
>1k	0.002894116871817131	2.248654491116422
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3104	0.17242097603381762	No Hit
GTCCTACAGTGGACATTTCTAAATT	2097	0.11648414521356816	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1966	0.10920735788739866	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1936	0.107540918041711	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1929	0.1071520820777172	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1917	0.10648550613944215	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	5.5547994856255673E-5
6	0.0	0.0	0.0	0.0	1.6664398456876702E-4
7	0.0	0.0	0.0	5.5547994856255673E-5	1.6664398456876702E-4
8	0.0	0.0	0.0	5.5547994856255673E-5	1.6664398456876702E-4
9	0.0	0.0	0.0	5.5547994856255673E-5	1.6664398456876702E-4
10	0.0	0.0	0.0	1.1109598971251135E-4	2.221919794250227E-4
11	0.0	0.0	0.0	1.1109598971251135E-4	2.221919794250227E-4
12	0.0	0.0	0.0	1.1109598971251135E-4	6.110279434188125E-4
13	0.0	0.0	0.0	1.1109598971251135E-4	6.665759382750681E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGTA	135	0.0	14.778644	9
GGTATCA	730	0.0	14.048584	1
CGCGTAA	145	0.0	13.10458	10
GCGTAAC	155	0.0	12.871721	11
TAACGTG	60	4.0905582E-4	12.667408	5
GCGATCG	75	1.4779982E-5	12.667408	14
GTATCAA	2015	0.0	12.299772	1
GCGCACT	55	0.0030674208	12.091618	11
GTCCTAC	1175	0.0	11.799039	1
TGTAGGA	1515	0.0	11.785535	2
AACCGCG	65	8.0186815E-4	11.693318	7
CGCACTC	65	8.020553E-4	11.692992	12
TAGAAAT	315	0.0	11.459714	4
GTAGGAC	1435	0.0	11.451403	3
CTGTAGG	1520	0.0	11.432446	1
GGACGTG	1050	0.0	11.401301	6
ACTCGTG	75	2.0730321E-4	11.400667	15
TGAGTTA	530	0.0	11.293115	12
AGGACGT	1120	0.0	11.197084	5
TAAGCTG	120	1.2744385E-7	11.083982	5
>>END_MODULE