##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062070_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1800245
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.311077658874208	32.0	32.0	32.0	32.0	32.0
2	31.50651716849651	32.0	32.0	32.0	32.0	32.0
3	31.61136900810723	32.0	32.0	32.0	32.0	32.0
4	31.680156312057527	32.0	32.0	32.0	32.0	32.0
5	31.659475238092593	32.0	32.0	32.0	32.0	32.0
6	35.3180183808315	36.0	36.0	36.0	36.0	36.0
7	35.322908270818694	36.0	36.0	36.0	36.0	36.0
8	35.276549025271564	36.0	36.0	36.0	36.0	36.0
9	35.3553466333749	36.0	36.0	36.0	36.0	36.0
10	35.24819399581724	36.0	36.0	36.0	36.0	36.0
11	35.35230704709637	36.0	36.0	36.0	36.0	36.0
12	35.28592108296371	36.0	36.0	36.0	36.0	36.0
13	35.30919402636863	36.0	36.0	36.0	36.0	36.0
14	35.27900202472441	36.0	36.0	36.0	36.0	36.0
15	35.25766770633997	36.0	36.0	36.0	36.0	36.0
16	35.268977833572656	36.0	36.0	36.0	36.0	36.0
17	35.25200625470422	36.0	36.0	36.0	36.0	36.0
18	35.25349271904658	36.0	36.0	36.0	36.0	36.0
19	35.23319770364589	36.0	36.0	36.0	36.0	36.0
20	35.228585553632975	36.0	36.0	36.0	36.0	36.0
21	35.21249329952312	36.0	36.0	36.0	36.0	36.0
22	35.19535563215007	36.0	36.0	36.0	36.0	36.0
23	35.16826709697847	36.0	36.0	36.0	36.0	36.0
24	35.157253040558366	36.0	36.0	36.0	36.0	36.0
25	35.13122936044816	36.0	36.0	36.0	36.0	36.0
26	35.096641290491014	36.0	36.0	36.0	36.0	36.0
27	35.07396048871126	36.0	36.0	36.0	36.0	36.0
28	35.05590072462359	36.0	36.0	36.0	36.0	36.0
29	35.02407783385039	36.0	36.0	36.0	36.0	36.0
30	34.99714649950423	36.0	36.0	36.0	36.0	36.0
31	34.99409858102647	36.0	36.0	36.0	36.0	36.0
32	34.96980522095603	36.0	36.0	36.0	36.0	36.0
33	34.95770797863624	36.0	36.0	36.0	36.0	36.0
34	34.95115942552264	36.0	36.0	36.0	36.0	36.0
35	34.93184205483143	36.0	36.0	36.0	36.0	36.0
36	34.898370499570895	36.0	36.0	36.0	36.0	36.0
37	34.87013489830551	36.0	36.0	36.0	32.0	36.0
38	34.8553963488303	36.0	36.0	36.0	32.0	36.0
39	34.79846965274171	36.0	36.0	36.0	32.0	36.0
40	34.81366758413438	36.0	36.0	36.0	32.0	36.0
41	34.79050073739963	36.0	36.0	36.0	32.0	36.0
42	34.75907501478965	36.0	36.0	36.0	32.0	36.0
43	34.76845484920108	36.0	36.0	36.0	32.0	36.0
44	34.70720207527309	36.0	36.0	36.0	32.0	36.0
45	34.69151920988532	36.0	36.0	36.0	32.0	36.0
46	34.63723604287194	36.0	36.0	36.0	32.0	36.0
47	34.62050887518088	36.0	36.0	36.0	32.0	36.0
48	34.59493291190921	36.0	36.0	36.0	32.0	36.0
49	34.569309732841916	36.0	36.0	36.0	32.0	36.0
50	34.192480467936306	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	19.0
21	39.0
22	130.0
23	308.0
24	810.0
25	2002.0
26	4138.0
27	8058.0
28	13653.0
29	21813.0
30	32474.0
31	47970.0
32	72455.0
33	124503.0
34	285422.0
35	1186446.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.79329965304712	17.720546234083177	12.216197654234003	26.2699564586357
2	15.703800155874772	19.35852475581378	38.4538511234816	26.48382396482985
3	19.130028557255883	22.739985657491793	28.373225170157585	29.75676061509474
4	12.57495507555916	15.481115070448745	35.26908837408242	36.67484147990968
5	14.561740207582858	36.64334576682618	33.26741637943724	15.527497646153718
6	35.322767358202555	34.80561523882319	16.1708635051693	13.700753897804965
7	30.994336881924404	29.89848603940019	19.6670453188316	19.440131759843798
8	27.829045491030385	34.49797110948787	18.56719502067774	19.105788378803997
9	27.058039322425557	14.199900569089207	18.491149815719528	40.25091029276571
10	16.617358535842918	26.73626463968218	30.89431210435919	25.75206472011572
11	37.4426814128077	20.762062941432973	22.409616468869515	19.385639176889814
12	24.945382434057585	23.02092215226261	28.923174345714052	23.11052106796575
13	30.00547147749334	19.427244625037147	25.08941838471986	25.477865512749652
14	23.10135564881447	19.95861674383209	24.748520340287016	32.19150726706643
15	24.885057311643692	28.51150815583435	21.546178436823876	25.057256095698083
16	25.264534907942576	25.83923765508392	24.58223509708455	24.31399233988896
17	23.634949687403658	26.591769453602147	25.47542140097598	24.297859458018216
18	24.47366769227009	24.935828036357353	27.084818088244848	23.505686183127704
19	25.910862132654167	24.572933128546392	25.042646973050893	24.473557765748552
20	26.00483936012905	24.66890488924242	25.0723515446291	24.25390420599943
21	26.151621669062635	24.17811141156421	24.472016015606773	25.19825090376638
22	25.88114395540607	24.882835391849444	25.037314365544688	24.198706287199798
23	24.200539371030054	24.84667364719802	25.85087029820941	25.101916683562514
24	25.191863733421616	24.705094220979916	25.704074354695717	24.398967690902747
25	24.870683908812158	24.803081811314048	25.723347998044705	24.60288628182909
26	23.989745811669554	25.797282584544284	26.13223792383238	24.080733679953784
27	24.593581515332975	25.187836274981418	25.587061266343675	24.631520943341936
28	24.223665623647737	25.266703069564162	25.403075063478248	25.106556243309857
29	24.375415223337384	24.803914597863834	25.34328832441969	25.477381854379093
30	24.02130615459905	25.167770136165856	25.931949844214436	24.878973865020658
31	25.40325223664871	24.468709137347393	25.085849759865088	25.042188866138808
32	24.758031724729424	25.161701021421635	25.045160745591133	25.035106508257805
33	24.195678027910677	25.026343665827333	25.930943767035895	24.847034539226094
34	24.935175021886067	25.077323367210447	25.958598669504816	24.028902941398663
35	25.000833222237034	25.136092965382396	25.93343109807581	23.92964271430476
36	24.011145186833527	25.439844376218936	25.68881131894154	24.860199118005998
37	24.98622393657678	25.034995644986402	25.02060854649199	24.958171871944828
38	24.63925743440476	25.32677496674064	25.20995753355793	24.82401006529667
39	25.640124050333373	24.335629142162947	25.12108127985371	24.90316552764997
40	25.912654062449313	24.672599936453057	25.440162555742447	23.974583445355186
41	24.89163890634035	24.75348996878734	26.106705195055525	24.248165929816786
42	25.390810963649486	25.686283550359494	25.3860891795039	23.536816306487122
43	24.650591272892104	24.99544491007586	25.777864929362774	24.57609888766926
44	24.189303291893033	25.431679054316792	25.342188003421878	25.036829650368297
45	24.36922741919984	25.67029568167767	25.26456591525558	24.69591098386691
46	24.53547189389706	24.793663420015623	25.371594710406654	25.299269975680673
47	25.29433820772403	24.801899762529683	25.48553653015595	24.418225499590328
48	25.161511088298656	25.441537707770195	24.551740764565437	24.845210439365708
49	25.071589546231987	25.312563708446817	24.798285517163993	24.817561228157203
50	24.696384162149158	26.051052677479795	24.521960939196845	24.730602221174202
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	217.0
1	152.0
2	87.0
3	216.0
4	345.0
5	352.0
6	359.0
7	289.5
8	220.0
9	294.5
10	369.0
11	643.0
12	917.0
13	1711.5
14	2506.0
15	3077.0
16	3648.0
17	3563.0
18	3478.0
19	3196.5
20	2915.0
21	3426.5
22	3938.0
23	4861.0
24	5784.0
25	7605.5
26	9427.0
27	13264.0
28	17101.0
29	20936.0
30	24771.0
31	29424.0
32	34077.0
33	40786.5
34	47496.0
35	55812.0
36	64128.0
37	70207.0
38	76286.0
39	79212.5
40	82139.0
41	85220.5
42	88302.0
43	90930.5
44	93559.0
45	101709.5
46	109860.0
47	117296.5
48	124733.0
49	130420.0
50	136107.0
51	133700.5
52	131294.0
53	130682.0
54	130070.0
55	130712.0
56	131354.0
57	126165.5
58	120977.0
59	111216.0
60	101455.0
61	89204.0
62	76953.0
63	66211.5
64	55470.0
65	46887.0
66	38304.0
67	32854.0
68	27404.0
69	24105.0
70	20806.0
71	16390.5
72	11975.0
73	10455.0
74	8935.0
75	6574.5
76	4214.0
77	3655.5
78	3097.0
79	2654.5
80	2212.0
81	1741.5
82	1271.0
83	1080.0
84	889.0
85	693.0
86	497.0
87	331.5
88	166.0
89	104.5
90	43.0
91	35.5
92	28.0
93	26.5
94	25.0
95	21.0
96	17.0
97	16.0
98	15.0
99	10.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03205119303205953
2	0.004554935578212966
3	1.1109598971251135E-4
4	0.0
5	0.0
6	5.5547994856255673E-5
7	0.0
8	0.0
9	0.0
10	5.5547994856255673E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.554799485625568E-4
17	0.0
18	2.221919794250227E-4
19	0.0
20	3.888359639937897E-4
21	5.5547994856255673E-5
22	0.0
23	0.0
24	4.99931953706301E-4
25	2.777399742812784E-4
26	2.777399742812784E-4
27	3.888359639937897E-4
28	0.001222055886837625
29	4.99931953706301E-4
30	7.776719279875794E-4
31	3.888359639937897E-4
32	4.99931953706301E-4
33	1.1109598971251135E-4
34	7.221239331313238E-4
35	2.777399742812784E-4
36	3.3328796913753404E-4
37	0.0011665078919813692
38	0.0
39	4.443839588500454E-4
40	5.5547994856255673E-5
41	7.776719279875794E-4
42	0.004332743598787943
43	0.003388427686231596
44	0.003610619665656619
45	0.0017775358354001815
46	0.001721987840543926
47	0.0011109598971251137
48	0.003277331696519085
49	0.0028884957325252952
50	0.0014442478662626476
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1800245.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.86036160776796
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.88825430446568	42.181174345330085
2	13.616052493414621	14.939631269181536
3	4.064874299054394	6.690014218087392
4	1.7473972952622314	3.8345138996208687
5	0.9527050621224284	2.613287210679373
6	0.5832652425661157	1.9198885272251762
7	0.38473308645059295	1.4774617371606529
8	0.28187174401315496	1.2370868642859107
9	0.20567209573452622	1.0154920990161223
>10	1.1300825480398524	11.130695985543088
>50	0.0792143869191886	2.9827027584460843
>100	0.05813985505722799	6.438780555727115
>500	0.0060068108829459035	2.2906649038761344
>1k	0.001730776017120006	1.2486056258205156
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6664398456876702E-4
2	0.0	0.0	0.0	0.0	1.6664398456876702E-4
3	0.0	0.0	0.0	0.0	1.6664398456876702E-4
4	0.0	0.0	0.0	0.0	1.6664398456876702E-4
5	0.0	0.0	0.0	0.0	1.6664398456876702E-4
6	0.0	0.0	0.0	0.0	1.6664398456876702E-4
7	0.0	0.0	0.0	0.0	1.6664398456876702E-4
8	0.0	0.0	0.0	0.0	1.6664398456876702E-4
9	0.0	0.0	0.0	0.0	1.6664398456876702E-4
10	0.0	0.0	0.0	0.0	2.221919794250227E-4
11	0.0	0.0	0.0	0.0	2.221919794250227E-4
12	0.0	0.0	0.0	0.0	3.3328796913753404E-4
13	0.0	0.0	0.0	0.0	3.888359639937897E-4
14	0.0	0.0	0.0	0.0	4.443839588500454E-4
15	0.0	0.0	0.0	5.5547994856255673E-5	6.110279434188125E-4
16	0.0	0.0	0.0	1.6664398456876702E-4	6.110279434188125E-4
17	0.0	0.0	0.0	2.221919794250227E-4	6.110279434188125E-4
18	0.0	0.0	0.0	3.3328796913753404E-4	6.110279434188125E-4
19	0.0	0.0	0.0	3.3328796913753404E-4	6.110279434188125E-4
20	0.0	0.0	0.0	3.3328796913753404E-4	7.221239331313238E-4
21	0.0	0.0	0.0	7.221239331313238E-4	7.221239331313238E-4
22	0.0	0.0	0.0	7.776719279875794E-4	7.221239331313238E-4
23	0.0	0.0	0.0	9.443159125563465E-4	9.443159125563465E-4
24	0.0	0.0	0.0	0.001222055886837625	9.443159125563465E-4
25	0.0	0.0	0.0	0.0013886998714063919	9.443159125563465E-4
26	0.0	0.0	0.0	0.0015553438559751589	0.001055411902268858
27	0.0	0.0	0.0	0.00205527580968146	0.001055411902268858
28	0.0	0.0	0.0	0.003055139717094062	0.001055411902268858
29	0.0	0.0	0.0	0.004388291593644199	0.001055411902268858
30	0.0	0.0	0.0	0.007387883315882005	0.001055411902268858
31	0.0	0.0	0.0	0.01322042277578885	0.001055411902268858
32	0.0	0.0	0.0	0.019219606220264463	0.0011109598971251135
33	0.0	0.0	0.0	0.02632974956186519	0.0011109598971251135
34	0.0	0.0	0.0	0.03355098889317843	0.0013331518765501362
35	0.0	0.0	0.0	0.04138325616791048	0.0013331518765501362
36	0.0	0.0	0.0	0.05193737519059906	0.0013331518765501362
37	0.0	0.0	0.0	0.06960163755488837	0.0013331518765501362
38	0.0	0.0	0.0	0.09120980755397182	0.0013331518765501362
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	955	0.0	28.80752	1
GTATCAA	2160	0.0	26.186571	1
TGTAGGA	1700	0.0	23.68343	2
GTAGGAC	1690	0.0	22.780201	3
TAGGACG	1335	0.0	22.24635	4
GTCCTAC	1510	0.0	21.717438	1
CTGTAGG	1700	0.0	21.102695	1
AGGACGT	1485	0.0	20.591812	5
TCCTACA	1630	0.0	20.381294	2
GGACGTG	1435	0.0	20.236166	6
GATATAC	600	0.0	20.17487	1
GTACGAT	55	0.004485433	19.999243	3
GTCCTAA	860	0.0	19.961603	1
TTAGGAC	910	0.0	19.339926	3
TAGGACC	1365	0.0	18.534098	4
CCTACAG	1730	0.0	18.438608	3
GACGTGA	845	0.0	17.70347	7
TACGACG	425	0.0	17.599333	5
ACTGTTC	745	0.0	17.42216	8
ACGACGG	435	0.0	17.194752	6
>>END_MODULE