FastQCFastQC Report
Thu 2 Feb 2017
SRR4062069_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062069_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309456
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23030.1758745616500287No Hit
TATCAACGCAGAGTACTTTTTTTTT13750.10500543737246612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4600.014.4491031
ATTACTC701.09236775E-412.212223
GTATCAA11000.011.5667891
CTAATAC1001.9325435E-611.3980723
CCCTAGA1501.7826096E-1011.3941511
CCTAATA600.005883887411.0810362
AAGTAGG700.001489900910.8582136
CTTATAC700.001493232710.8553073
GTCCTAC1252.2540553E-710.634541
CCGCCCA909.498309E-510.5579
GGCGTGT909.501993E-510.5565978
CAATACC909.516746E-510.5549824
TTTAGAC909.5278236E-510.5537713
GGGTAAA909.5611256E-510.5501391
CTTATTC1201.5258356E-610.2899263
TAAGAGT1303.8407234E-710.2302134
TAGTGTC750.002644084910.1347217
GTAAATC750.002650805710.131623
CTCTATA750.002651646710.1312322
GTCTAGT856.622627E-410.0536621
Produced by FastQC (version 0.11.5)