##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062069_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1309456 Sequences flagged as poor quality 0 Sequence length 25 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.27853704133625 32.0 32.0 32.0 32.0 32.0 2 30.842893537468996 32.0 32.0 32.0 32.0 32.0 3 30.872787630894052 32.0 32.0 32.0 32.0 32.0 4 30.88569833579746 32.0 32.0 32.0 32.0 32.0 5 30.814272491782848 32.0 32.0 32.0 32.0 32.0 6 34.45082308989382 36.0 36.0 36.0 32.0 36.0 7 34.40695449102528 36.0 36.0 36.0 32.0 36.0 8 34.35950119744383 36.0 36.0 36.0 32.0 36.0 9 34.48911150890141 36.0 36.0 36.0 32.0 36.0 10 34.19946603780501 36.0 36.0 36.0 32.0 36.0 11 34.46331377304774 36.0 36.0 36.0 32.0 36.0 12 34.311175785975244 36.0 36.0 36.0 32.0 36.0 13 34.37383615791596 36.0 36.0 36.0 32.0 36.0 14 34.29941059493408 36.0 36.0 36.0 32.0 36.0 15 34.25673638518591 36.0 36.0 36.0 32.0 36.0 16 34.25940695983676 36.0 36.0 36.0 32.0 36.0 17 34.20766638970687 36.0 36.0 36.0 32.0 36.0 18 34.201203400496084 36.0 36.0 36.0 32.0 36.0 19 34.20613063745555 36.0 36.0 36.0 32.0 36.0 20 34.196777898608275 36.0 36.0 36.0 32.0 36.0 21 34.17932561307902 36.0 36.0 36.0 32.0 36.0 22 34.14330225681504 36.0 36.0 36.0 32.0 36.0 23 34.11385415012035 36.0 36.0 36.0 32.0 36.0 24 34.090973656235875 36.0 36.0 36.0 32.0 36.0 25 33.746959042533696 36.0 36.0 36.0 32.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 17.0 5 89.0 6 274.0 7 51.0 8 242.0 9 199.0 10 106.0 11 22.0 12 51.0 13 50.0 14 179.0 15 310.0 16 420.0 17 622.0 18 837.0 19 1132.0 20 1659.0 21 2438.0 22 3473.0 23 5270.0 24 7398.0 25 10681.0 26 14336.0 27 18426.0 28 24668.0 29 32750.0 30 43016.0 31 58754.0 32 82249.0 33 118013.0 34 264378.0 35 617346.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.29486605745043 16.71933541157226 11.80373013848647 26.182068392490848 2 16.746356308540573 18.6645589195505 37.56455930138875 27.024525470520178 3 19.52845779290205 21.928018523836 28.01776015542427 30.525763527837686 4 13.547050292018914 15.040558818879537 34.153878348747035 37.258512540354516 5 15.16558532584989 35.77752262565287 33.59108281519882 15.465809233298422 6 35.79579753977727 33.84365099620834 16.003903223292188 14.356648240722203 7 31.117616208752985 29.878734039023453 19.474723134108135 19.52892661811543 8 28.04580442142025 33.95239740794314 18.279566604179216 19.722231566457392 9 26.69960034590303 15.196566048440188 18.19653317083129 39.907300434825494 10 16.56880888290713 27.227303315795915 31.333965496145844 24.869922305151107 11 36.85752546113778 21.408845799992505 21.68966970306237 20.043959035807344 12 24.462636118928177 23.175776948488927 28.612504588278476 23.74908234430442 13 29.864035413036117 19.82233838290766 24.40205327095729 25.911572933098938 14 23.21163150586897 19.01756714601161 25.117975741190428 32.65282560692899 15 25.065324266062657 26.780234410307507 21.937100589046324 26.217340734583512 16 25.76671496347156 26.013907950622574 23.076417269102407 25.14295981680347 17 24.278807370245733 26.03786973380466 24.888381165607253 24.794941730342355 18 24.564575676789424 25.999998470921128 25.060150140254763 24.375275712034693 19 25.56581511240623 25.226406310557525 24.980717146336413 24.22706143069983 20 25.639189127211022 25.004357065325404 24.945422023818622 24.41103178364495 21 25.60919304757813 24.6967985164416 24.77675534281298 24.917253093167286 22 25.590544258615537 24.486171643325715 24.771629717913296 25.151654380145448 23 24.958306263614478 24.824855734321854 25.020369167271756 25.19646883479191 24 24.86577345371936 24.758759122971696 24.896501854405475 25.478965568903472 25 25.06439841560788 24.56151749420223 25.11821026675085 25.255873823439035 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 86.0 1 86.0 2 220.0 3 354.0 4 354.0 5 354.0 6 724.0 7 1094.0 8 1094.0 9 1094.0 10 1469.5 11 1845.0 12 1845.0 13 1845.0 14 2562.0 15 3279.0 16 3279.0 17 3279.0 18 5604.5 19 7930.0 20 7930.0 21 7930.0 22 11854.0 23 15778.0 24 15778.0 25 15778.0 26 23411.5 27 31045.0 28 31045.0 29 31045.0 30 40930.0 31 50815.0 32 50815.0 33 50815.0 34 62867.5 35 74920.0 36 74920.0 37 74920.0 38 90795.0 39 106670.0 40 106670.0 41 106670.0 42 121213.5 43 135757.0 44 135757.0 45 135757.0 46 148986.5 47 162216.0 48 162216.0 49 162216.0 50 171414.5 51 180613.0 52 180613.0 53 180613.0 54 175439.0 55 170265.0 56 170265.0 57 170265.0 58 156551.5 59 142838.0 60 142838.0 61 142838.0 62 122605.5 63 102373.0 64 102373.0 65 102373.0 66 82526.5 67 62680.0 68 62680.0 69 62680.0 70 47592.0 71 32504.0 72 32504.0 73 32504.0 74 23770.0 75 15036.0 76 15036.0 77 15036.0 78 10951.5 79 6867.0 80 6867.0 81 6867.0 82 4737.5 83 2608.0 84 2608.0 85 2608.0 86 1815.0 87 1022.0 88 1022.0 89 1022.0 90 631.0 91 240.0 92 240.0 93 240.0 94 175.0 95 110.0 96 110.0 97 110.0 98 310.5 99 511.0 100 511.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.001069146271428746 2 7.636759081633899E-5 3 6.109407265307119E-4 4 0.009393213670409697 5 0.03306716682347478 6 0.06063586710817316 7 0.10859471414083406 8 0.11149668259185495 9 0.12027895553573392 10 0.13562884128981806 11 0.1431892327806356 12 0.13562884128981806 13 0.12898486088879657 14 0.11913344167348884 15 0.1335669163377769 16 0.11997348517246856 17 0.1265410979826737 18 0.11294766681736537 19 0.09920150047042436 20 0.094390342248995 21 0.09545948852042375 22 0.10561637809899684 23 0.08446255544287093 24 0.09316846079593358 25 0.09118290343470876 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1309456.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.94924275678527 #Duplication Level Percentage of deduplicated Percentage of total 1 61.04808309660559 26.219689407525127 2 16.3044096991979 14.005241003538698 3 7.945621538797846 10.237752849701112 4 4.448563728134452 7.6424977391470525 5 2.7891099733346443 5.9895080660060245 6 1.800814585485251 4.64061736751794 7 1.2480433394767656 3.75217614507236 8 0.9194298008532257 3.159105097173434 9 0.6611981462043648 2.55581637225009 >10 2.764156176154155 18.716335052802375 >50 0.05572964280385051 1.6060800735384246 >100 0.014125083459993915 1.0704095730077063 >500 1.7879737297381626E-4 0.045460933714677236 >1k 5.363921189214489E-4 0.359310319005025 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2303 0.1758745616500287 No Hit TATCAACGCAGAGTACTTTTTTTTT 1375 0.10500543737246612 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 7.6367590816339E-5 0.0 11 0.0 0.0 0.0 7.6367590816339E-5 0.0 12 0.0 0.0 0.0 7.6367590816339E-5 2.2910277244901698E-4 13 0.0 0.0 0.0 7.6367590816339E-5 3.05470363265356E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 460 0.0 14.449103 1 ATTACTC 70 1.09236775E-4 12.21222 3 GTATCAA 1100 0.0 11.566789 1 CTAATAC 100 1.9325435E-6 11.398072 3 CCCTAGA 150 1.7826096E-10 11.394151 1 CCTAATA 60 0.0058838874 11.081036 2 AAGTAGG 70 0.0014899009 10.858213 6 CTTATAC 70 0.0014932327 10.855307 3 GTCCTAC 125 2.2540553E-7 10.63454 1 CCGCCCA 90 9.498309E-5 10.557 9 GGCGTGT 90 9.501993E-5 10.556597 8 CAATACC 90 9.516746E-5 10.554982 4 TTTAGAC 90 9.5278236E-5 10.553771 3 GGGTAAA 90 9.5611256E-5 10.550139 1 CTTATTC 120 1.5258356E-6 10.289926 3 TAAGAGT 130 3.8407234E-7 10.230213 4 TAGTGTC 75 0.0026440849 10.134721 7 GTAAATC 75 0.0026508057 10.13162 3 CTCTATA 75 0.0026516467 10.131232 2 GTCTAGT 85 6.622627E-4 10.053662 1 >>END_MODULE