FastQCFastQC Report
Thu 2 Feb 2017
SRR4062069_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062069_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309456
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4650.032.1801151
TAGCCCG300.005747794629.3306085
GTATCAA11350.025.9800851
CTATAGA1452.9467628E-1019.7290861
TACCGTA808.995712E-519.2482137
GTATTAT1408.349707E-817.290051
CGTGCGG902.2171198E-417.10952410
CTACTAG1451.2458804E-716.6938421
TATCAAC17300.016.5302562
ATCAACG18050.016.0871493
CCGATAG1105.6207762E-516.00034735
TCAACGC18150.015.9985154
GTGTAAG2650.015.7775431
GTATAGG1401.5822334E-615.7182271
CAACGCA18800.015.6793955
AACGCAG18950.015.6713686
CCGTGCG1552.670531E-715.6114539
GTCCTAA1603.8132384E-715.1287951
ACTATAC2355.0931703E-1114.9773323
TAGGACA2800.014.9271854
Produced by FastQC (version 0.11.5)