##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062069_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1309456
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.307736953360784	32.0	32.0	32.0	32.0	32.0
2	31.476562022702556	32.0	32.0	32.0	32.0	32.0
3	31.582551074644737	32.0	32.0	32.0	32.0	32.0
4	31.66073086839115	32.0	32.0	32.0	32.0	32.0
5	31.631131553866645	32.0	32.0	32.0	32.0	32.0
6	35.27612535281827	36.0	36.0	36.0	36.0	36.0
7	35.29079862171772	36.0	36.0	36.0	36.0	36.0
8	35.24075646680759	36.0	36.0	36.0	36.0	36.0
9	35.32685787074938	36.0	36.0	36.0	36.0	36.0
10	35.214026282670055	36.0	36.0	36.0	36.0	36.0
11	35.32450880365587	36.0	36.0	36.0	36.0	36.0
12	35.25234448503806	36.0	36.0	36.0	36.0	36.0
13	35.28091589179018	36.0	36.0	36.0	36.0	36.0
14	35.25033601739959	36.0	36.0	36.0	36.0	36.0
15	35.23190469935607	36.0	36.0	36.0	36.0	36.0
16	35.24597619163989	36.0	36.0	36.0	36.0	36.0
17	35.229029459561836	36.0	36.0	36.0	36.0	36.0
18	35.224784185188355	36.0	36.0	36.0	36.0	36.0
19	35.21698399946237	36.0	36.0	36.0	36.0	36.0
20	35.21157182830122	36.0	36.0	36.0	36.0	36.0
21	35.20880350313413	36.0	36.0	36.0	36.0	36.0
22	35.18858976552095	36.0	36.0	36.0	36.0	36.0
23	35.159240936694324	36.0	36.0	36.0	36.0	36.0
24	35.14885876272284	36.0	36.0	36.0	36.0	36.0
25	35.13832003519018	36.0	36.0	36.0	36.0	36.0
26	35.08750122188145	36.0	36.0	36.0	36.0	36.0
27	35.08510786158527	36.0	36.0	36.0	36.0	36.0
28	35.06046556738065	36.0	36.0	36.0	36.0	36.0
29	35.049345682481885	36.0	36.0	36.0	36.0	36.0
30	35.02551212106401	36.0	36.0	36.0	36.0	36.0
31	35.02986354638873	36.0	36.0	36.0	36.0	36.0
32	35.0061086435894	36.0	36.0	36.0	36.0	36.0
33	34.98800876089002	36.0	36.0	36.0	36.0	36.0
34	34.980408658251974	36.0	36.0	36.0	36.0	36.0
35	34.97226176366369	36.0	36.0	36.0	36.0	36.0
36	34.94502908077858	36.0	36.0	36.0	36.0	36.0
37	34.93556637260053	36.0	36.0	36.0	36.0	36.0
38	34.93246355738566	36.0	36.0	36.0	36.0	36.0
39	34.90710188047556	36.0	36.0	36.0	36.0	36.0
40	34.89838146528024	36.0	36.0	36.0	36.0	36.0
41	34.86819259295463	36.0	36.0	36.0	32.0	36.0
42	34.859418720445746	36.0	36.0	36.0	32.0	36.0
43	34.84242082208184	36.0	36.0	36.0	32.0	36.0
44	34.80893668821251	36.0	36.0	36.0	32.0	36.0
45	34.792555839982406	36.0	36.0	36.0	32.0	36.0
46	34.769320236800624	36.0	36.0	36.0	32.0	36.0
47	34.74607546950795	36.0	36.0	36.0	32.0	36.0
48	34.72637263107733	36.0	36.0	36.0	32.0	36.0
49	34.7025612162608	36.0	36.0	36.0	32.0	36.0
50	34.29761977492944	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	12.0
21	32.0
22	79.0
23	235.0
24	571.0
25	1487.0
26	3009.0
27	5786.0
28	9783.0
29	15455.0
30	23089.0
31	34083.0
32	51620.0
33	86951.0
34	200193.0
35	877067.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.909269668500606	17.217204905647115	12.196357280473514	26.677168145378765
2	16.19378996591561	19.082218761765912	37.394999377576084	27.328991894742387
3	18.99952193815132	22.290359187875254	28.220006201057846	30.490112672915583
4	13.377081780525653	15.364548331520874	34.234139978739265	37.024229909214206
5	14.920875418304117	35.99523160034641	33.405297169660024	15.678595811689453
6	36.011247453133066	33.86134046914282	15.94865638450283	14.178755693221287
7	31.274361261470414	29.814747498197725	19.532920541049105	19.377970699282756
8	28.139777128822963	34.0543706699576	18.230318544494814	19.575533656724627
9	26.88925782920541	14.96041104092081	18.059407876247846	40.09092325362593
10	16.415137278381252	27.180600188169745	31.553943011449032	24.850319521999975
11	37.19903532459281	21.181238621228967	21.66808201268313	19.951644041495094
12	24.49078090443665	23.082027956647647	28.6162345279261	23.810956610989603
13	30.003528182695717	19.557205434928704	24.51162925672951	25.92763712564607
14	23.295399005388497	18.795209613763273	25.22864456690412	32.68074681394411
15	25.227499053041875	26.640757688689042	21.919331386468883	26.212411871800196
16	25.801061514376265	26.000763679407385	23.03707663522853	25.161098170987817
17	24.37004374335602	25.940161410539947	24.899500250485698	24.790294595618334
18	24.721963632087036	25.837851129977373	25.14481259703494	24.295372640900652
19	25.639425837905204	25.101874366148998	25.09805598660818	24.160643809337618
20	25.702450874450438	24.880885195398683	24.94488148086488	24.471782449285996
21	25.713598405443488	24.551359153235506	24.911508986563113	24.823533454757897
22	25.82331899659095	24.2582415904009	24.874757151061207	25.043682261946948
23	25.25178394692147	24.528659229481555	24.95532495937244	25.264231864224534
24	24.949520559871214	24.67329645254016	24.963495957062694	25.413687030525935
25	25.253980097032887	24.40393049616901	25.183339913689153	25.158749493108957
26	25.06987655904913	24.86711998607051	24.963267076609146	25.099736378271214
27	25.175588853649355	24.95396925887261	24.869430013036	25.001011874442035
28	25.412507732372102	24.57710606905295	25.016533911702037	24.993852286872915
29	25.1197642974477	24.98222532107065	24.808411489735743	25.089598891745908
30	25.497886873259745	24.920538186728006	24.750464704597558	24.83111023541469
31	25.224292984524777	24.774408758499764	24.854366114576525	25.14693214239894
32	25.097311774171004	24.99826263067499	24.833384372534752	25.07104122261925
33	25.09545978012176	24.79159220803817	24.934170935628032	25.178777076212032
34	25.17268740162832	24.70432174803562	24.958245732740615	25.164745117595444
35	25.088032941999444	24.706728687474847	25.22518944933495	24.980048921190757
36	25.24477740739468	24.54830444727005	25.21552859777541	24.991389547559862
37	25.314200399408904	24.59702085250509	25.05912855544567	25.029650192640336
38	25.293709754279643	24.743863100402	24.885677716547942	25.076749428770423
39	25.382833071645454	24.988468423335636	24.53132923185953	25.097369273159376
40	25.48554514241028	24.76203858701629	24.864294791119367	24.888121479454064
41	25.15628746813538	24.76215132200946	24.866928456767212	25.214632753087944
42	25.128723669117164	24.723956272901816	24.8508852186584	25.296434839322618
43	25.007789827401865	24.541087521001987	25.052925003818544	25.39819764777761
44	25.098250657168215	24.771384887498606	24.975752364049043	25.15461209128414
45	25.11463020494445	24.862382658687608	24.872921611573908	25.15006552479403
46	25.263264007844654	24.768467428883014	24.825057066946076	25.143211496326256
47	24.96023106078252	24.919679343596762	25.050880567937874	25.06920902768285
48	25.160797119933036	24.928708567343865	24.952536019929525	24.95795829279357
49	25.226400367800363	24.83973649120288	24.805522504097276	25.12834063689948
50	25.09929824909751	25.26440712396683	24.63627553479009	25.00001909214557
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	22.0
1	27.0
2	32.0
3	91.0
4	150.0
5	165.0
6	180.0
7	152.0
8	124.0
9	178.0
10	232.0
11	392.5
12	553.0
13	973.0
14	1393.0
15	1729.5
16	2066.0
17	2041.0
18	2016.0
19	2029.0
20	2042.0
21	2489.0
22	2936.0
23	3939.5
24	4943.0
25	6043.0
26	7143.0
27	8932.5
28	10722.0
29	12751.5
30	14781.0
31	17363.0
32	19945.0
33	23169.5
34	26394.0
35	30884.5
36	35375.0
37	39613.5
38	43852.0
39	49285.5
40	54719.0
41	59626.5
42	64534.0
43	68401.0
44	72268.0
45	78452.5
46	84637.0
47	87992.0
48	91347.0
49	94020.5
50	96694.0
51	99763.0
52	102832.0
53	103840.5
54	104849.0
55	101941.0
56	99033.0
57	95600.5
58	92168.0
59	85158.5
60	78149.0
61	69361.5
62	60574.0
63	53240.5
64	45907.0
65	38320.5
66	30734.0
67	25464.5
68	20195.0
69	16701.0
70	13207.0
71	10764.0
72	8321.0
73	7103.0
74	5885.0
75	4653.5
76	3422.0
77	2812.0
78	2202.0
79	1814.5
80	1427.0
81	1141.0
82	855.0
83	591.5
84	328.0
85	233.5
86	139.0
87	98.5
88	58.0
89	38.0
90	18.0
91	15.5
92	13.0
93	15.5
94	18.0
95	13.0
96	8.0
97	9.5
98	11.0
99	7.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033143534414291125
2	0.004505687858164
3	1.5273518163267798E-4
4	0.0
5	1.5273518163267798E-4
6	3.0547036326535596E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.5820554489803397E-4
17	0.0
18	3.8183795408169505E-4
19	0.0
20	3.8183795408169505E-4
21	7.636759081633899E-5
22	0.0
23	0.0
24	9.164110897960679E-4
25	2.2910277244901698E-4
26	3.0547036326535596E-4
27	3.8183795408169505E-4
28	0.001985557361224814
29	6.87308317347051E-4
30	0.0016800869979594578
31	6.109407265307119E-4
32	3.8183795408169505E-4
33	3.0547036326535596E-4
34	6.87308317347051E-4
35	2.2910277244901698E-4
36	7.636759081633899E-5
37	0.001603719407143119
38	0.0
39	6.109407265307119E-4
40	0.0
41	7.636759081633901E-4
42	0.0047347906306130175
43	0.004276585085714984
44	0.00381837954081695
45	0.002138292542857492
46	0.002214660133673831
47	0.001298249043877763
48	0.003207438814286238
49	0.00381837954081695
50	0.001298249043877763
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1309456.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.387852273025736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.04581700111348	25.028253683833096
2	17.055305195607612	14.458755142055674
3	8.321577129599502	10.582013461441598
4	4.788605184372997	8.11914756596191
5	2.9264017057810983	6.202194159808985
6	1.9205741109528194	4.884540701667951
7	1.363260606844186	4.044998244878274
8	0.9723426177237238	3.2972412191072267
9	0.7064915585229523	2.6951993833329557
>10	2.8444089602065743	18.804171997349325
>50	0.04742293018476688	1.3273067205928308
>100	0.007611769418939003	0.49403085337140107
>500	1.8122967139971703E-4	0.06214686659873184
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	7.6367590816339E-5	0.0
11	0.0	0.0	0.0	7.6367590816339E-5	0.0
12	0.0	0.0	0.0	7.6367590816339E-5	7.6367590816339E-5
13	0.0	0.0	0.0	7.6367590816339E-5	7.6367590816339E-5
14	0.0	0.0	0.0	7.6367590816339E-5	7.6367590816339E-5
15	0.0	0.0	0.0	1.52735181632678E-4	1.52735181632678E-4
16	0.0	0.0	0.0	1.52735181632678E-4	1.52735181632678E-4
17	0.0	0.0	0.0	3.05470363265356E-4	1.52735181632678E-4
18	0.0	0.0	0.0	4.5820554489803397E-4	1.52735181632678E-4
19	0.0	0.0	0.0	4.5820554489803397E-4	1.52735181632678E-4
20	0.0	0.0	0.0	9.92778680612407E-4	1.52735181632678E-4
21	0.0	0.0	0.0	0.001069146271428746	1.52735181632678E-4
22	0.0	0.0	0.0	0.001450984225510441	2.2910277244901698E-4
23	0.0	0.0	0.0	0.0018328221795921359	2.2910277244901698E-4
24	0.0	0.0	0.0	0.002672865678571865	2.2910277244901698E-4
25	0.0	0.0	0.0	0.003589276768367933	2.2910277244901698E-4
26	0.0	0.0	0.0	0.004811158221429357	4.5820554489803397E-4
27	0.0	0.0	0.0	0.00610940726530712	4.5820554489803397E-4
28	0.0	0.0	0.0	0.0076367590816339	4.5820554489803397E-4
29	0.0	0.0	0.0	0.010004154396940409	4.5820554489803397E-4
30	0.0	0.0	0.0	0.013593431165308341	4.5820554489803397E-4
31	0.0	0.0	0.0	0.019550103248982785	5.34573135714373E-4
32	0.0	0.0	0.0	0.02688139196735133	5.34573135714373E-4
33	0.0	0.0	0.0	0.033907210322454515	5.34573135714373E-4
34	0.0	0.0	0.0	0.0413148666316394	6.10940726530712E-4
35	0.0	0.0	0.0	0.05086081548368177	6.10940726530712E-4
36	0.0	0.0	0.0	0.062010483742867266	6.10940726530712E-4
37	0.0	0.0	0.0	0.07926955926735989	6.10940726530712E-4
38	0.0	0.0	0.0	0.10172163096736354	6.10940726530712E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	465	0.0	32.180115	1
TAGCCCG	30	0.0057477946	29.330608	5
GTATCAA	1135	0.0	25.980085	1
CTATAGA	145	2.9467628E-10	19.729086	1
TACCGTA	80	8.995712E-5	19.248213	7
GTATTAT	140	8.349707E-8	17.29005	1
CGTGCGG	90	2.2171198E-4	17.109524	10
CTACTAG	145	1.2458804E-7	16.693842	1
TATCAAC	1730	0.0	16.530256	2
ATCAACG	1805	0.0	16.087149	3
CCGATAG	110	5.6207762E-5	16.000347	35
TCAACGC	1815	0.0	15.998515	4
GTGTAAG	265	0.0	15.777543	1
GTATAGG	140	1.5822334E-6	15.718227	1
CAACGCA	1880	0.0	15.679395	5
AACGCAG	1895	0.0	15.671368	6
CCGTGCG	155	2.670531E-7	15.611453	9
GTCCTAA	160	3.8132384E-7	15.128795	1
ACTATAC	235	5.0931703E-11	14.977332	3
TAGGACA	280	0.0	14.927185	4
>>END_MODULE