FastQCFastQC Report
Thu 2 Feb 2017
SRR4062068_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062068_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1397570
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20700.14811422683658063No Hit
TATCAACGCAGAGTACTTTTTTTTT15590.11155076311025566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6700.015.5911051
GCGTAAC902.2810127E-914.78064911
CGCGTAA954.8858055E-914.0027210
TCGCGTA1001.00408215E-813.3025839
CTAGCGG500.001498434113.30115318
GCTCGTA751.4753776E-512.6691279
AATCGAG1051.9912477E-812.66731219
TTAGGGG853.9481565E-612.2925414
CTTACAC1302.6248017E-911.6891343
CGCAAGA2550.011.5450572
AAGACGG2750.011.3997645
CAAGACG2750.011.3985394
GTATTAT1001.9377385E-611.395681
CGTAACT1201.2724195E-711.08469312
TTAGATC600.00588075811.0819124
GTATCAA15800.010.8787871
CGTTATT1502.1427695E-910.7629722
ATAGGGC1158.8430534E-710.7362163
TTACTCT803.774625E-410.686134
CGTCGTA2350.010.51268110
Produced by FastQC (version 0.11.5)