##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062068_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1397570
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.226245554784377	32.0	32.0	32.0	32.0	32.0
2	30.70783216583069	32.0	32.0	32.0	32.0	32.0
3	30.73434819007277	32.0	32.0	32.0	32.0	32.0
4	30.746346157974198	32.0	32.0	32.0	32.0	32.0
5	30.626433023032835	32.0	32.0	32.0	32.0	32.0
6	34.30979414269053	36.0	36.0	36.0	32.0	36.0
7	34.23019168986169	36.0	36.0	36.0	32.0	36.0
8	34.17030631739376	36.0	36.0	36.0	32.0	36.0
9	34.33702569459848	36.0	36.0	36.0	32.0	36.0
10	33.96844093676882	36.0	36.0	36.0	32.0	36.0
11	34.32038967636684	36.0	36.0	36.0	32.0	36.0
12	34.10453072118033	36.0	36.0	36.0	32.0	36.0
13	34.21928347059539	36.0	36.0	36.0	32.0	36.0
14	34.10487989868128	36.0	36.0	36.0	32.0	36.0
15	34.051812073813835	36.0	36.0	36.0	32.0	36.0
16	34.05600506593588	36.0	36.0	36.0	32.0	36.0
17	33.981298253396965	36.0	36.0	36.0	32.0	36.0
18	33.99137073634952	36.0	36.0	36.0	32.0	36.0
19	33.99341070572493	36.0	36.0	36.0	32.0	36.0
20	33.98249747776498	36.0	36.0	36.0	32.0	36.0
21	33.97315053986562	36.0	36.0	36.0	32.0	36.0
22	33.943539858468625	36.0	36.0	36.0	32.0	36.0
23	33.89728171039733	36.0	36.0	36.0	32.0	36.0
24	33.87813919875212	36.0	36.0	36.0	32.0	36.0
25	33.509698977510965	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	13.0
5	116.0
6	285.0
7	81.0
8	242.0
9	185.0
10	93.0
11	22.0
12	57.0
13	53.0
14	189.0
15	252.0
16	484.0
17	622.0
18	849.0
19	1284.0
20	1891.0
21	2856.0
22	4320.0
23	6440.0
24	9273.0
25	12953.0
26	17990.0
27	22972.0
28	30393.0
29	39302.0
30	51634.0
31	69524.0
32	97774.0
33	136591.0
34	291228.0
35	597599.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.51959426196664	17.190344259104492	11.588048244358708	25.702013234570163
2	17.118547531923358	18.831454113794983	37.24782944061318	26.802168913668478
3	19.697931327603836	22.003015248686463	27.89570130291085	30.40335212079885
4	13.412483845252185	15.052217583521657	34.06587272849576	37.469425842730395
5	15.487503820394572	35.80155278013781	32.848450405015505	15.862492994452108
6	35.921988100607855	33.589363575831776	16.100622176400258	14.388026147160113
7	31.543412160347255	29.644544246630783	19.332065511301167	19.4799780817208
8	28.58832344336933	33.10979435942546	18.447982418690742	19.85389977851447
9	26.78191872322958	14.960344495331675	18.441618543488524	39.81611823795022
10	17.571487833938875	26.222319980913174	29.92225557003626	26.283936615111696
11	37.26216970303188	21.36492414222026	21.20291938167347	20.169986773074395
12	24.62890068050996	23.40759379760095	27.846527736978388	24.116977784910706
13	29.688344068148766	19.171210156401123	24.741898736897912	26.398547038552195
14	23.595943474551444	19.338482907620705	24.41307962260015	32.6524939952277
15	25.436624279577103	26.717837828542933	21.630312357599156	26.215225534280812
16	26.190056594311912	25.671896267640946	22.866179525753992	25.271867612293143
17	24.52767334166275	25.982407927465296	24.444212010407952	25.045706720464
18	25.184186566934315	25.020863135680944	25.132897278874044	24.662053018510704
19	25.89968557738449	24.972532785182743	24.57939708210083	24.548384555331936
20	25.914644627152146	24.431489386083022	24.462070644856333	25.191795341908502
21	26.613311561585757	24.090295458370484	24.306365729550492	24.990027250493267
22	26.215833501057556	23.94228090142758	24.529327625218368	25.312557972296496
23	24.931467806339622	24.146462678957107	25.048624200099685	25.87344531460359
24	24.91351959148302	24.63685390361463	24.912230442536185	25.537396062366163
25	25.29243210841169	24.28713742751415	24.748139198614634	25.672291265459528
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	233.0
1	233.0
2	321.0
3	409.0
4	409.0
5	409.0
6	866.5
7	1324.0
8	1324.0
9	1324.0
10	1544.5
11	1765.0
12	1765.0
13	1765.0
14	2506.0
15	3247.0
16	3247.0
17	3247.0
18	5380.0
19	7513.0
20	7513.0
21	7513.0
22	11901.5
23	16290.0
24	16290.0
25	16290.0
26	23941.0
27	31592.0
28	31592.0
29	31592.0
30	40540.5
31	49489.0
32	49489.0
33	49489.0
34	62713.5
35	75938.0
36	75938.0
37	75938.0
38	91119.0
39	106300.0
40	106300.0
41	106300.0
42	123606.0
43	140912.0
44	140912.0
45	140912.0
46	159513.5
47	178115.0
48	178115.0
49	178115.0
50	186695.0
51	195275.0
52	195275.0
53	195275.0
54	186421.0
55	177567.0
56	177567.0
57	177567.0
58	164749.0
59	151931.0
60	151931.0
61	151931.0
62	133320.0
63	114709.0
64	114709.0
65	114709.0
66	93090.0
67	71471.0
68	71471.0
69	71471.0
70	54192.5
71	36914.0
72	36914.0
73	36914.0
74	27760.5
75	18607.0
76	18607.0
77	18607.0
78	14603.0
79	10599.0
80	10599.0
81	10599.0
82	7376.0
83	4153.0
84	4153.0
85	4153.0
86	3009.5
87	1866.0
88	1866.0
89	1866.0
90	1266.5
91	667.0
92	667.0
93	667.0
94	415.5
95	164.0
96	164.0
97	164.0
98	342.0
99	520.0
100	520.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0012879497985789621
2	2.8621106635088044E-4
3	5.008693661140408E-4
4	0.010589809454982576
5	0.03312893093011441
6	0.0615353792654393
7	0.10554033071688716
8	0.11033436607826443
9	0.11970777850125575
10	0.13187174882116817
11	0.13823994504747528
12	0.13251572372045764
13	0.1287949798578962
14	0.11426976824058903
15	0.13323125138633485
16	0.11949312020149258
17	0.12264144193135228
18	0.11212318524295743
19	0.09731176255929935
20	0.09230306889815895
21	0.09051424973346595
22	0.10224890345385204
23	0.08199947050952726
24	0.09301859656403615
25	0.0903711442002905
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1397570.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.998855981739524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.17521085509513	45.92071651708522
2	12.255956099382018	14.216628654531597
3	3.6805866718534697	6.40409448927418
4	1.555499642397983	3.608687989563518
5	0.8386318840770705	2.4319844933140446
6	0.5290402610154726	1.8410237944306973
7	0.3574212949407632	1.4511018347053322
8	0.24865654904223872	1.1537436301453725
9	0.19964449820322294	1.0421237248951845
>10	1.037348684206803	10.717507906338382
>50	0.06474501926017645	2.603619692840142
>100	0.05119883507639658	5.8894303937276025
>500	0.0049466983259104785	1.9054652370285876
>1k	0.0011130071233298579	0.8138716421201305
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2070	0.14811422683658063	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1559	0.11155076311025566	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.155276658772011E-5
2	0.0	0.0	0.0	0.0	7.155276658772011E-5
3	0.0	0.0	0.0	0.0	7.155276658772011E-5
4	0.0	0.0	0.0	0.0	7.155276658772011E-5
5	0.0	0.0	0.0	0.0	7.155276658772011E-5
6	7.155276658772011E-5	0.0	0.0	0.0	7.155276658772011E-5
7	7.155276658772011E-5	0.0	0.0	0.0	7.155276658772011E-5
8	7.155276658772011E-5	0.0	0.0	0.0	7.155276658772011E-5
9	7.155276658772011E-5	0.0	0.0	0.0	7.155276658772011E-5
10	7.155276658772011E-5	0.0	0.0	0.0	7.155276658772011E-5
11	7.155276658772011E-5	0.0	0.0	0.0	7.155276658772011E-5
12	7.155276658772011E-5	0.0	0.0	0.0	3.5776383293860056E-4
13	7.155276658772011E-5	0.0	0.0	0.0	3.5776383293860056E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	670	0.0	15.591105	1
GCGTAAC	90	2.2810127E-9	14.780649	11
CGCGTAA	95	4.8858055E-9	14.00272	10
TCGCGTA	100	1.00408215E-8	13.302583	9
CTAGCGG	50	0.0014984341	13.301153	18
GCTCGTA	75	1.4753776E-5	12.669127	9
AATCGAG	105	1.9912477E-8	12.667312	19
TTAGGGG	85	3.9481565E-6	12.292541	4
CTTACAC	130	2.6248017E-9	11.689134	3
CGCAAGA	255	0.0	11.545057	2
AAGACGG	275	0.0	11.399764	5
CAAGACG	275	0.0	11.398539	4
GTATTAT	100	1.9377385E-6	11.39568	1
CGTAACT	120	1.2724195E-7	11.084693	12
TTAGATC	60	0.005880758	11.081912	4
GTATCAA	1580	0.0	10.878787	1
CGTTATT	150	2.1427695E-9	10.762972	2
ATAGGGC	115	8.8430534E-7	10.736216	3
TTACTCT	80	3.774625E-4	10.68613	4
CGTCGTA	235	0.0	10.512681	10
>>END_MODULE