FastQCFastQC Report
Thu 2 Feb 2017
SRR4062068_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062068_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1397570
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7600.028.0963121
GTATCAA15450.024.792021
CCGAACG450.001398826524.4429329
CCTATTC3050.023.080543
CTATTCC2950.022.3714984
CGCAATA2750.021.60021236
CGTCCGA654.9347494E-420.30643810
TACGCGT1001.1232023E-619.80090340
ACGCGTA1001.1232023E-619.80090341
ATACGAA3000.019.80090140
CAATACG3050.019.47629738
TACTAGT808.989179E-519.2501872
TACGAAT3100.019.16216341
TCTAGCG3150.018.85800228
CGAATGC3150.018.85800243
CGCGTAT1203.1601485E-718.33482642
AATACGA3250.018.27775439
GTACTAG851.4242753E-418.128851
CTAGCGG3300.017.9995329
GCGCAAT3450.017.21694435
Produced by FastQC (version 0.11.5)