##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062068_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1397570
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27547886689039	32.0	32.0	32.0	32.0	32.0
2	31.45226929599233	32.0	32.0	32.0	32.0	32.0
3	31.576062737465744	32.0	32.0	32.0	32.0	32.0
4	31.656985338838126	32.0	32.0	32.0	32.0	32.0
5	31.626002990905643	32.0	32.0	32.0	32.0	32.0
6	35.2813919875212	36.0	36.0	36.0	36.0	36.0
7	35.285728085176416	36.0	36.0	36.0	36.0	36.0
8	35.234849059438886	36.0	36.0	36.0	36.0	36.0
9	35.32072812095279	36.0	36.0	36.0	36.0	36.0
10	35.203611268129684	36.0	36.0	36.0	36.0	36.0
11	35.32234521347768	36.0	36.0	36.0	36.0	36.0
12	35.24648353928605	36.0	36.0	36.0	36.0	36.0
13	35.28091258398506	36.0	36.0	36.0	36.0	36.0
14	35.24852923288279	36.0	36.0	36.0	36.0	36.0
15	35.227493435033665	36.0	36.0	36.0	36.0	36.0
16	35.23345020285209	36.0	36.0	36.0	36.0	36.0
17	35.21414741300973	36.0	36.0	36.0	36.0	36.0
18	35.21059195603798	36.0	36.0	36.0	36.0	36.0
19	35.200151691865166	36.0	36.0	36.0	36.0	36.0
20	35.2069806879083	36.0	36.0	36.0	36.0	36.0
21	35.19662056283406	36.0	36.0	36.0	36.0	36.0
22	35.182166188455675	36.0	36.0	36.0	36.0	36.0
23	35.15012128193936	36.0	36.0	36.0	36.0	36.0
24	35.133860915732306	36.0	36.0	36.0	36.0	36.0
25	35.11095544409225	36.0	36.0	36.0	36.0	36.0
26	35.07678828251894	36.0	36.0	36.0	36.0	36.0
27	35.06665712629779	36.0	36.0	36.0	36.0	36.0
28	35.04426969668782	36.0	36.0	36.0	36.0	36.0
29	35.033103887461806	36.0	36.0	36.0	36.0	36.0
30	35.01209170202566	36.0	36.0	36.0	36.0	36.0
31	35.01268988315433	36.0	36.0	36.0	36.0	36.0
32	34.989356525970074	36.0	36.0	36.0	36.0	36.0
33	34.9718740385097	36.0	36.0	36.0	36.0	36.0
34	34.96053220947788	36.0	36.0	36.0	36.0	36.0
35	34.940242706984264	36.0	36.0	36.0	36.0	36.0
36	34.92596220582869	36.0	36.0	36.0	36.0	36.0
37	34.91566361613372	36.0	36.0	36.0	36.0	36.0
38	34.901526220511315	36.0	36.0	36.0	32.0	36.0
39	34.89077183969318	36.0	36.0	36.0	32.0	36.0
40	34.87591748534957	36.0	36.0	36.0	32.0	36.0
41	34.8502715427492	36.0	36.0	36.0	32.0	36.0
42	34.82316735476577	36.0	36.0	36.0	32.0	36.0
43	34.81754187625665	36.0	36.0	36.0	32.0	36.0
44	34.77569924941148	36.0	36.0	36.0	32.0	36.0
45	34.75862103508232	36.0	36.0	36.0	32.0	36.0
46	34.7460127220819	36.0	36.0	36.0	32.0	36.0
47	34.719678441866954	36.0	36.0	36.0	32.0	36.0
48	34.6978298045894	36.0	36.0	36.0	32.0	36.0
49	34.6843485478366	36.0	36.0	36.0	32.0	36.0
50	34.27231265696888	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	12.0
21	22.0
22	94.0
23	258.0
24	576.0
25	1394.0
26	3101.0
27	5819.0
28	10106.0
29	16262.0
30	24861.0
31	36884.0
32	56799.0
33	97026.0
34	225510.0
35	918844.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.10435091315638	17.69947292023946	12.027782628985664	26.168393537618496
2	16.501358506065515	19.20255658175974	37.02828855427334	27.267796357901407
3	19.051350026653502	22.415057618071433	28.2608680097169	30.272724345558167
4	13.261088890001933	15.486451483646615	34.1318145066079	37.12064511974356
5	15.17391985655091	36.11349421745903	32.71373363318734	15.998852292802718
6	35.99526892091757	33.783353499331334	15.991505237315993	14.229872342435105
7	31.623389168342193	29.541990741072006	19.403822348791117	19.430797741794688
8	28.68099630072197	33.19747848050545	18.33732836280115	19.784196855971437
9	26.879154532509997	14.692001116223159	18.41260187325143	40.016242478015414
10	17.36642887297237	26.281044956603246	30.102964431119727	26.24956173930465
11	37.54237712601158	21.197220890545733	21.18877766408838	20.07162431935431
12	24.67239565817812	23.323268244166663	27.92983535708408	24.074500740571132
13	29.836716586646823	18.959837432114313	24.867806263729186	26.33563971750968
14	23.684037293301945	19.13092009702555	24.477128158160237	32.707914451512266
15	25.55685940596893	26.619561095329754	21.616734761049536	26.206844737651778
16	26.333681559289047	25.487635593462894	22.894902544434586	25.283780302813476
17	24.621593193900843	25.844787738717915	24.552687879676867	24.98093118770437
18	25.345600357191923	24.796432087740993	25.189686655676148	24.668280899390943
19	25.957125582260637	24.871312349291983	24.732499982111808	24.439062086335568
20	26.141557739037353	24.166764455867494	24.530039411433037	25.161638393662116
21	26.669218715341632	23.951429982040256	24.354987585594998	25.024363717023117
22	26.329414626816543	23.815909042123113	24.64835392860465	25.20632240245569
23	24.996887454653436	23.987850340233404	25.210973332283892	25.804288872829268
24	25.07606135281878	24.50363348588536	24.92594214471549	25.494363016580373
25	25.506898774799346	24.032837066129925	24.87937966480319	25.580884494267536
26	25.054326710137577	24.85111583520743	25.116434822616224	24.978122632038772
27	25.578935919929247	24.454193153229475	24.73804419690261	25.228826729938664
28	25.35319102654371	24.214836885278746	25.088583973026967	25.343388115150578
29	25.079602908493502	24.63296798281579	25.090264331743423	25.197164776947282
30	25.18482370551852	24.62405584638238	25.05853108444218	25.13258936365692
31	25.51575455578421	24.363034537237652	24.499486248930282	25.62172465804786
32	25.524377451590695	24.35534165926091	24.4303293878912	25.68995150125719
33	24.99593937177967	24.26423920999852	24.902849022622885	25.836972395598924
34	25.68546399180856	24.275145843783115	24.92349149265468	25.11589867175364
35	25.860441753418616	24.292144848869498	25.00101962911259	24.846393768599288
36	25.05643744655884	24.673414116695824	24.775520279915426	25.49462815682991
37	25.853977212963553	24.416102762765384	24.64443107182646	25.0854889524446
38	25.2418125746832	24.2104509970878	24.971843986347732	25.575892441881265
39	25.762937924579877	24.263608379759553	24.45930687670923	25.514146818951346
40	25.849581774079294	24.40443054730711	24.780440335725583	24.965547342888012
41	25.093288054191866	24.695235171760928	25.045418671062926	25.16605810298429
42	25.94626388912741	24.650804009456913	24.700034919599055	24.70289718181662
43	25.53183572590836	24.066237429500013	24.86093202377358	25.540994820818053
44	25.154488834442922	24.43449501685853	24.75857094608132	25.652445202617223
45	25.386177465611098	24.707987727041154	24.631925721122197	25.273909086225547
46	25.3684320493666	24.42334552615159	24.71571556959924	25.492506854882564
47	25.41166537274025	24.49806984340509	24.98935641173335	25.10090837212131
48	25.92214141171285	24.917567555963902	24.150559424346767	25.009731607976484
49	25.315987417747387	24.927443106522677	24.228635787292383	25.52793368843755
50	25.365156620402402	24.98034061011011	24.411416828437744	25.243085941049735
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	102.0
1	75.0
2	48.0
3	120.0
4	192.0
5	208.5
6	225.0
7	179.5
8	134.0
9	171.0
10	208.0
11	383.5
12	559.0
13	1078.5
14	1598.0
15	1968.0
16	2338.0
17	2318.5
18	2299.0
19	2194.5
20	2090.0
21	2472.0
22	2854.0
23	3480.0
24	4106.0
25	5509.5
26	6913.0
27	8616.0
28	10319.0
29	12038.5
30	13758.0
31	15881.0
32	18004.0
33	21036.5
34	24069.0
35	27916.0
36	31763.0
37	37705.5
38	43648.0
39	49263.0
40	54878.0
41	60471.5
42	66065.0
43	70515.5
44	74966.0
45	81907.0
46	88848.0
47	95946.0
48	103044.0
49	107658.0
50	112272.0
51	110800.0
52	109328.0
53	110122.5
54	110917.0
55	110533.5
56	110150.0
57	105791.0
58	101432.0
59	94273.5
60	87115.0
61	76188.5
62	65262.0
63	56475.0
64	47688.0
65	39865.0
66	32042.0
67	27523.0
68	23004.0
69	20331.0
70	17658.0
71	13782.5
72	9907.0
73	8572.0
74	7237.0
75	5452.0
76	3667.0
77	3161.0
78	2655.0
79	2261.5
80	1868.0
81	1475.5
82	1083.0
83	884.5
84	686.0
85	517.5
86	349.0
87	230.5
88	112.0
89	75.0
90	38.0
91	29.0
92	20.0
93	20.5
94	21.0
95	17.0
96	13.0
97	13.5
98	14.0
99	9.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03134011176542141
2	0.00565266856042989
3	3.577638329386006E-4
4	0.0
5	7.155276658772011E-5
6	2.1465829976316037E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	7.155276658772012E-4
17	0.0
18	1.4310553317544022E-4
19	0.0
20	4.2931659952632074E-4
21	0.0
22	0.0
23	0.0
24	0.0010017387322280816
25	2.1465829976316037E-4
26	5.008693661140408E-4
27	4.2931659952632074E-4
28	0.0018603719312807229
29	5.724221327017609E-4
30	0.0015741608649298426
31	4.2931659952632074E-4
32	5.724221327017609E-4
33	2.1465829976316037E-4
34	9.301859656403614E-4
35	2.1465829976316037E-4
36	3.577638329386006E-4
37	0.0015026080983421223
38	0.0
39	8.586331990526415E-4
40	0.0
41	0.001216397031991242
42	0.005294904727491288
43	0.0037207438625614458
44	0.004150060462087767
45	0.0018603719312807229
46	0.0018603719312807229
47	0.0010732914988158017
48	0.004436271528438647
49	0.0032914272630351253
50	0.0013595025651666822
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1397570.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.14944935081742
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.73148636054376	46.569240647975555
2	12.838009358921418	15.18722368681685
3	3.6062755286731036	6.399276351850262
4	1.5665724319656806	3.706475868757636
5	0.8384277352141426	2.479626942918474
6	0.5098249878788127	1.8093520378991363
7	0.3630799642527711	1.503318596910612
8	0.2621927381779619	1.2406844869607592
9	0.20331094758702567	1.0823157537088925
>10	0.9726321762583643	10.152524587568529
>50	0.05844299304518527	2.400901363194597
>100	0.04512306079525059	5.391432497740083
>500	0.0036487236999173784	1.447001144467903
>1k	9.729929866446342E-4	0.6306260332308659
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4310553317544022E-4
2	0.0	0.0	0.0	0.0	1.4310553317544022E-4
3	0.0	0.0	0.0	0.0	1.4310553317544022E-4
4	0.0	0.0	0.0	0.0	1.4310553317544022E-4
5	0.0	0.0	0.0	0.0	2.1465829976316034E-4
6	7.155276658772011E-5	0.0	0.0	0.0	2.1465829976316034E-4
7	7.155276658772011E-5	0.0	0.0	0.0	2.1465829976316034E-4
8	7.155276658772011E-5	0.0	0.0	7.155276658772011E-5	2.1465829976316034E-4
9	7.155276658772011E-5	0.0	0.0	7.155276658772011E-5	2.1465829976316034E-4
10	7.155276658772011E-5	0.0	0.0	7.155276658772011E-5	2.8621106635088044E-4
11	7.155276658772011E-5	0.0	0.0	7.155276658772011E-5	2.8621106635088044E-4
12	7.155276658772011E-5	0.0	0.0	7.155276658772011E-5	3.5776383293860056E-4
13	7.155276658772011E-5	0.0	0.0	7.155276658772011E-5	5.008693661140408E-4
14	7.155276658772011E-5	0.0	0.0	7.155276658772011E-5	5.008693661140408E-4
15	7.155276658772011E-5	0.0	0.0	1.4310553317544022E-4	7.155276658772011E-4
16	7.155276658772011E-5	0.0	0.0	2.1465829976316034E-4	7.155276658772011E-4
17	7.155276658772011E-5	0.0	0.0	3.5776383293860056E-4	7.870804324649213E-4
18	7.155276658772011E-5	0.0	0.0	5.008693661140408E-4	7.870804324649213E-4
19	7.155276658772011E-5	0.0	0.0	5.724221327017609E-4	7.870804324649213E-4
20	7.155276658772011E-5	0.0	0.0	7.870804324649213E-4	7.870804324649213E-4
21	7.155276658772011E-5	0.0	0.0	8.586331990526414E-4	7.870804324649213E-4
22	7.155276658772011E-5	0.0	0.0	9.301859656403614E-4	8.586331990526414E-4
23	7.155276658772011E-5	0.0	0.0	0.0013595025651666822	9.301859656403614E-4
24	7.155276658772011E-5	0.0	0.0	0.0017172663981052827	0.0010017387322280816
25	7.155276658772011E-5	0.0	0.0	0.0020034774644561632	0.0010732914988158017
26	7.155276658772011E-5	0.0	0.0	0.002361241297394764	0.001216397031991242
27	7.155276658772011E-5	0.0	0.0	0.0027905578969210846	0.0012879497985789621
28	7.155276658772011E-5	0.0	0.0	0.0035060855627982858	0.0012879497985789621
29	7.155276658772011E-5	7.155276658772011E-5	0.0	0.005080246427728128	0.0012879497985789621
30	7.155276658772011E-5	7.155276658772011E-5	0.0	0.0074414877251228915	0.0012879497985789621
31	7.155276658772011E-5	7.155276658772011E-5	0.0	0.01151999542062294	0.0012879497985789621
32	7.155276658772011E-5	7.155276658772011E-5	0.0	0.016385583548587906	0.0012879497985789621
33	7.155276658772011E-5	7.155276658772011E-5	0.0	0.020607196777263394	0.0012879497985789621
34	7.155276658772011E-5	7.155276658772011E-5	0.0	0.02690384023698276	0.0014310553317544022
35	7.155276658772011E-5	7.155276658772011E-5	0.0	0.03549017222750918	0.0014310553317544022
36	7.155276658772011E-5	7.155276658772011E-5	0.0	0.04450582081756191	0.0014310553317544022
37	7.155276658772011E-5	7.155276658772011E-5	0.0	0.05895947966828138	0.0014310553317544022
38	7.155276658772011E-5	7.155276658772011E-5	0.0	0.07849338494672896	0.0014310553317544022
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	760	0.0	28.096312	1
GTATCAA	1545	0.0	24.79202	1
CCGAACG	45	0.0013988265	24.442932	9
CCTATTC	305	0.0	23.08054	3
CTATTCC	295	0.0	22.371498	4
CGCAATA	275	0.0	21.600212	36
CGTCCGA	65	4.9347494E-4	20.306438	10
TACGCGT	100	1.1232023E-6	19.800903	40
ACGCGTA	100	1.1232023E-6	19.800903	41
ATACGAA	300	0.0	19.800901	40
CAATACG	305	0.0	19.476297	38
TACTAGT	80	8.989179E-5	19.250187	2
TACGAAT	310	0.0	19.162163	41
TCTAGCG	315	0.0	18.858002	28
CGAATGC	315	0.0	18.858002	43
CGCGTAT	120	3.1601485E-7	18.334826	42
AATACGA	325	0.0	18.277754	39
GTACTAG	85	1.4242753E-4	18.12885	1
CTAGCGG	330	0.0	17.99953	29
GCGCAAT	345	0.0	17.216944	35
>>END_MODULE