FastQCFastQC Report
Thu 2 Feb 2017
SRR4062067_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062067_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414587
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42541.026081377370733No Hit
TATCAACGCAGAGTACTTTTTTTTT27670.6674111826950676No Hit
GGTATCAACGCAGAGTACTTTTTTT18770.45273971446282685No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13280.32031877507013No Hit
GTACTTTTTTTTTTTTTTTTTTTTT8060.19441034089346748No Hit
GAGTACTTTTTTTTTTTTTTTTTTT7490.18066171877072845No Hit
GTATCAACGCAGAGTACATGGGTAG6420.15485290180348152No Hit
CTTCAGACTCGCTTTCGCTTCGGCT6150.14834039658744727No Hit
GGTATCAACGCAGAGTACATGGGGG5040.1215667640326397No Hit
GTATCAACGCAGAGTACATGGGGTG4980.12011954065129878No Hit
ATACTATCTCGCCCTCTTCAGACTC4770.11505425881660543No Hit
GGTATCAACGCAGAGTACATGGGGT4640.11191860815703339No Hit
AACCAGTACCGTGAGGGAAAGGTGA4630.11167740426014322No Hit
GTACATGGGTAGGTCACTTGGTTTC4320.10420008345654833No Hit
GTGTAAAGGTATAAGGGAGCTTGAC4230.1020292483845369No Hit
GCAGAGTACTTTTTTTTTTTTTTTT4180.10082322890008612No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCTC307.7034015E-419.0022956
AGGCGTC359.917248E-519.015
TATCCTT453.5287805E-516.888895
CTCGCAC453.539764E-516.8827721
TTATCCT402.7575236E-416.6229934
ACCTCGA350.002164838416.2876828
TGCCACT551.1217531E-515.54921111
TCGCTTC551.124325E-515.54545515
TGCGCTA456.73517E-414.78134710
GCCACTT456.741343E-414.77956212
AATAGGC456.75989E-414.7742092
AAGCGAC551.950689E-413.8215211
CAGGCGT551.9546917E-413.81818214
TAGGCCC551.9566956E-413.8165134
GGATTAT551.960709E-413.8131771
CATGCCG852.682882E-713.41176415
CGTTCTA500.00149494813.30160712
CGACAAG500.001496309613.29999915
CCGACAA500.001496309613.29999914
ATAGGCC500.00149903613.2967893
Produced by FastQC (version 0.11.5)