##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062067_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	414587
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.07907146147853	32.0	32.0	32.0	32.0	32.0
2	30.776353334764476	32.0	32.0	32.0	32.0	32.0
3	30.827635695282293	32.0	32.0	32.0	32.0	32.0
4	30.782832071434946	32.0	32.0	32.0	32.0	32.0
5	30.82724976904727	32.0	32.0	32.0	32.0	32.0
6	34.414692211767374	36.0	36.0	36.0	32.0	36.0
7	34.31120609184562	36.0	36.0	36.0	32.0	36.0
8	34.23799829710049	36.0	36.0	36.0	32.0	36.0
9	34.281168970565886	36.0	36.0	36.0	32.0	36.0
10	34.1208021476795	36.0	36.0	36.0	32.0	36.0
11	34.35409455675166	36.0	36.0	36.0	32.0	36.0
12	34.24917568568238	36.0	36.0	36.0	32.0	36.0
13	34.29429046255671	36.0	36.0	36.0	32.0	36.0
14	34.225935690217014	36.0	36.0	36.0	32.0	36.0
15	34.21256575821239	36.0	36.0	36.0	32.0	36.0
16	34.19487104033653	36.0	36.0	36.0	32.0	36.0
17	34.141555330967925	36.0	36.0	36.0	32.0	36.0
18	34.14550625079899	36.0	36.0	36.0	32.0	36.0
19	34.12173319472149	36.0	36.0	36.0	32.0	36.0
20	34.069334060161076	36.0	36.0	36.0	32.0	36.0
21	34.05446142787883	36.0	36.0	36.0	32.0	36.0
22	34.04816600616999	36.0	36.0	36.0	32.0	36.0
23	34.036525506106074	36.0	36.0	36.0	32.0	36.0
24	34.02925320861484	36.0	36.0	36.0	32.0	36.0
25	33.72451620528381	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	13.0
5	30.0
6	91.0
7	20.0
8	74.0
9	60.0
10	32.0
11	5.0
12	9.0
13	21.0
14	134.0
15	177.0
16	244.0
17	308.0
18	489.0
19	644.0
20	845.0
21	1177.0
22	1603.0
23	2109.0
24	2787.0
25	3734.0
26	4848.0
27	6068.0
28	7743.0
29	10025.0
30	13090.0
31	17586.0
32	24987.0
33	35802.0
34	82133.0
35	197698.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.928036123044116	19.370065825178553	12.769698709305494	22.932199342471833
2	15.990409709927494	19.69941097866305	40.242796428244084	24.067382883165376
3	19.346233073312398	25.38339821796412	28.993299274932344	26.277069433791144
4	12.431008529854491	15.776371145161914	36.852300358948625	34.94031996603496
5	13.457761262456868	38.29669666771228	32.3675892189272	15.87795285090365
6	30.40358376396589	37.81054420208876	17.28210314424119	14.50376888970416
7	28.48373916991046	32.94786102713197	18.817552230732822	19.750847572224746
8	28.595644394433673	32.99664600502737	17.906566248041088	20.50114335249787
9	28.026391417959108	14.316523133546177	19.78655989026116	37.87052555823355
10	17.549037841441294	26.904692319397512	29.39202979587795	26.154240043283245
11	35.63291414436656	24.10658640397706	20.546942561610148	19.713556890046235
12	24.095070167387263	25.85589720055071	28.243520687906088	21.80551194415594
13	29.174767951037246	21.88783877457859	25.752060845979386	23.185332428404774
14	23.28816743660394	20.504318973781885	26.995457651840244	29.212055937773933
15	25.958470504976827	27.027986638294376	24.106623125991803	22.906919730736995
16	24.244414767087758	28.25061642231147	23.647674500039848	23.857294310560924
17	22.494397109681408	27.142139531289246	25.50643366371066	24.857029695318687
18	21.98903192622268	26.743554264436348	28.686028625175375	22.581385184165597
19	24.66535319000985	24.93263603175523	28.807392169361222	21.594618608873695
20	26.27164476035037	24.232474142176983	27.314890822525033	22.18099027494761
21	27.688675031567563	23.019814530011516	26.717142574319574	22.574367864101344
22	25.850632526265716	24.44552836890564	26.812883783711992	22.890955321116646
23	24.245482065865517	25.610156480091156	28.220250194332724	21.924111259710603
24	22.726427690175598	25.562372188139058	28.202509518739298	23.508690602946043
25	23.301456084482357	25.838437636560734	27.57989632852318	23.280209950433736
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	256.0
1	256.0
2	303.5
3	351.0
4	351.0
5	351.0
6	782.5
7	1214.0
8	1214.0
9	1214.0
10	1330.0
11	1446.0
12	1446.0
13	1446.0
14	1308.0
15	1170.0
16	1170.0
17	1170.0
18	2131.0
19	3092.0
20	3092.0
21	3092.0
22	5369.0
23	7646.0
24	7646.0
25	7646.0
26	10595.0
27	13544.0
28	13544.0
29	13544.0
30	17011.5
31	20479.0
32	20479.0
33	20479.0
34	23048.5
35	25618.0
36	25618.0
37	25618.0
38	30382.0
39	35146.0
40	35146.0
41	35146.0
42	42037.0
43	48928.0
44	48928.0
45	48928.0
46	55017.5
47	61107.0
48	61107.0
49	61107.0
50	61989.5
51	62872.0
52	62872.0
53	62872.0
54	56649.5
55	50427.0
56	50427.0
57	50427.0
58	43774.0
59	37121.0
60	37121.0
61	37121.0
62	30102.0
63	23083.0
64	23083.0
65	23083.0
66	17253.5
67	11424.0
68	11424.0
69	11424.0
70	8344.5
71	5265.0
72	5265.0
73	5265.0
74	3898.0
75	2531.0
76	2531.0
77	2531.0
78	1785.0
79	1039.0
80	1039.0
81	1039.0
82	724.0
83	409.0
84	409.0
85	409.0
86	304.5
87	200.0
88	200.0
89	200.0
90	124.5
91	49.0
92	49.0
93	49.0
94	39.5
95	30.0
96	30.0
97	30.0
98	85.0
99	140.0
100	140.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.648155875606327E-4
2	2.4120389689015817E-4
3	0.001206019484450791
4	0.010371767566276802
5	0.03786901181175483
6	0.06608986774790333
7	0.11167740426014322
8	0.1095065691881318
9	0.12373759910465114
10	0.13748622122739015
11	0.14785798879366696
12	0.13917464850562125
13	0.13459177446470827
14	0.11674268609483654
15	0.13700381343360984
16	0.1215667640326397
17	0.12422000689843145
18	0.11481305491971529
19	0.10106443279697627
20	0.09624035485917311
21	0.09551674316850263
22	0.1095065691881318
23	0.0841801600146652
24	0.0974463743436239
25	0.0950343353747223
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	414587.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.558044051784194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.89657318086421	26.067395447502477
2	15.07374012263627	11.92575351592785
3	6.752305265308036	8.013239674084488
4	3.530634322128254	5.586599521819627
5	2.2141343730032865	4.37934125319168
6	1.4320042284431278	3.39883718106567
7	0.943190191795115	2.611753140936936
8	0.6933272762810813	2.194133674994445
9	0.4956794647145549	1.7647299090668855
>10	2.6854034359723102	19.185703937338932
>50	0.18337460400891112	4.903180292389155
>100	0.09413229672457435	6.694903636239813
>500	0.0030562434001485178	0.8018022413237222
>1k	0.0024449947201188147	2.47262657411833
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4254	1.026081377370733	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2767	0.6674111826950676	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1877	0.45273971446282685	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1328	0.32031877507013	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	806	0.19441034089346748	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	749	0.18066171877072845	No Hit
GTATCAACGCAGAGTACATGGGTAG	642	0.15485290180348152	No Hit
CTTCAGACTCGCTTTCGCTTCGGCT	615	0.14834039658744727	No Hit
GGTATCAACGCAGAGTACATGGGGG	504	0.1215667640326397	No Hit
GTATCAACGCAGAGTACATGGGGTG	498	0.12011954065129878	No Hit
ATACTATCTCGCCCTCTTCAGACTC	477	0.11505425881660543	No Hit
GGTATCAACGCAGAGTACATGGGGT	464	0.11191860815703339	No Hit
AACCAGTACCGTGAGGGAAAGGTGA	463	0.11167740426014322	No Hit
GTACATGGGTAGGTCACTTGGTTTC	432	0.10420008345654833	No Hit
GTGTAAAGGTATAAGGGAGCTTGAC	423	0.1020292483845369	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	418	0.10082322890008612	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCACCTC	30	7.7034015E-4	19.002295	6
AGGCGTC	35	9.917248E-5	19.0	15
TATCCTT	45	3.5287805E-5	16.88889	5
CTCGCAC	45	3.539764E-5	16.882772	1
TTATCCT	40	2.7575236E-4	16.622993	4
ACCTCGA	35	0.0021648384	16.287682	8
TGCCACT	55	1.1217531E-5	15.549211	11
TCGCTTC	55	1.124325E-5	15.545455	15
TGCGCTA	45	6.73517E-4	14.781347	10
GCCACTT	45	6.741343E-4	14.779562	12
AATAGGC	45	6.75989E-4	14.774209	2
AAGCGAC	55	1.950689E-4	13.82152	11
CAGGCGT	55	1.9546917E-4	13.818182	14
TAGGCCC	55	1.9566956E-4	13.816513	4
GGATTAT	55	1.960709E-4	13.813177	1
CATGCCG	85	2.682882E-7	13.411764	15
CGTTCTA	50	0.001494948	13.301607	12
CGACAAG	50	0.0014963096	13.299999	15
CCGACAA	50	0.0014963096	13.299999	14
ATAGGCC	50	0.001499036	13.296789	3
>>END_MODULE