FastQCFastQC Report
Thu 2 Feb 2017
SRR4062067_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062067_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414587
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12210.2945099581028831No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8010.1932043214090167No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6210.1497876199687882No Hit
CTTCAGACTCGCTTTCGCTTCGGCTCCGTCTTTTCTGACTTAACCTTGCA4770.11505425881660543No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGA250.002351332235.19903611
CCTACAC551.3703539E-731.9991233
TACTGCG300.005741484529.3325315
CCGTTAT551.5914522E-423.9993429
GGTATCA9850.023.9064711
GTATCAA20600.023.3961731
ATCCTAC1053.0431693E-923.055331
AGCGACC500.00257801621.99939710
TTACGGC803.6069214E-621.99939718
AATACGT500.00257801621.9993973
TAGGACC904.0695159E-721.9993954
GTAGCCC1158.731149E-921.0429043
ATAGGAC550.004479439519.9994533
GGCCACG550.004479439519.99945311
ATTGGGC550.004479439519.99945334
ACTGGAT550.004479439519.9994533
TACGGCC808.9723224E-519.24947219
GGACCGC808.9723224E-519.2494726
CGCACAA808.9723224E-519.24947227
TCGGTCG808.9723224E-519.2494729
Produced by FastQC (version 0.11.5)