##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062067_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	414587
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.173430425941962	32.0	32.0	32.0	32.0	32.0
2	31.47871255007996	32.0	32.0	32.0	32.0	32.0
3	31.59522368043378	32.0	32.0	32.0	32.0	32.0
4	31.672384807048942	32.0	32.0	32.0	32.0	32.0
5	31.655073603369136	32.0	32.0	32.0	32.0	32.0
6	35.28448311210916	36.0	36.0	36.0	36.0	36.0
7	35.28379568100302	36.0	36.0	36.0	36.0	36.0
8	35.25093165005174	36.0	36.0	36.0	36.0	36.0
9	35.29052526972626	36.0	36.0	36.0	36.0	36.0
10	35.22606111624339	36.0	36.0	36.0	36.0	36.0
11	35.321606803879526	36.0	36.0	36.0	36.0	36.0
12	35.25953539305381	36.0	36.0	36.0	36.0	36.0
13	35.27331295964417	36.0	36.0	36.0	36.0	36.0
14	35.235017016934925	36.0	36.0	36.0	36.0	36.0
15	35.22808722897727	36.0	36.0	36.0	36.0	36.0
16	35.2327931170056	36.0	36.0	36.0	36.0	36.0
17	35.21985252793744	36.0	36.0	36.0	36.0	36.0
18	35.20728580490946	36.0	36.0	36.0	36.0	36.0
19	35.18398068439194	36.0	36.0	36.0	36.0	36.0
20	35.1708447201673	36.0	36.0	36.0	36.0	36.0
21	35.145129972719836	36.0	36.0	36.0	36.0	36.0
22	35.14356938350696	36.0	36.0	36.0	36.0	36.0
23	35.1313427579736	36.0	36.0	36.0	36.0	36.0
24	35.116479773847225	36.0	36.0	36.0	36.0	36.0
25	35.10905069382301	36.0	36.0	36.0	36.0	36.0
26	35.075786264402886	36.0	36.0	36.0	36.0	36.0
27	35.0625996473599	36.0	36.0	36.0	36.0	36.0
28	35.044625132963645	36.0	36.0	36.0	36.0	36.0
29	35.00844937250806	36.0	36.0	36.0	36.0	36.0
30	34.99584164481762	36.0	36.0	36.0	36.0	36.0
31	34.980735044755384	36.0	36.0	36.0	36.0	36.0
32	34.948816533079906	36.0	36.0	36.0	36.0	36.0
33	34.91137204012668	36.0	36.0	36.0	36.0	36.0
34	34.887864308335764	36.0	36.0	36.0	36.0	36.0
35	34.84303656409873	36.0	36.0	36.0	32.0	36.0
36	34.82691931970853	36.0	36.0	36.0	32.0	36.0
37	34.817268751793954	36.0	36.0	36.0	32.0	36.0
38	34.809977157990964	36.0	36.0	36.0	32.0	36.0
39	34.73597339038609	36.0	36.0	36.0	32.0	36.0
40	34.722960440148874	36.0	36.0	36.0	32.0	36.0
41	34.666255816028965	36.0	36.0	36.0	32.0	36.0
42	34.62809494750197	36.0	36.0	36.0	32.0	36.0
43	34.57282789860753	36.0	36.0	36.0	32.0	36.0
44	34.480827908255684	36.0	36.0	36.0	32.0	36.0
45	34.404894509475696	36.0	36.0	36.0	32.0	36.0
46	34.320019682237984	36.0	36.0	36.0	32.0	36.0
47	34.233977428139326	36.0	36.0	36.0	32.0	36.0
48	34.17717632246067	36.0	36.0	36.0	32.0	36.0
49	34.073687790499946	36.0	36.0	36.0	32.0	36.0
50	33.668607554023644	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	6.0
21	11.0
22	23.0
23	91.0
24	241.0
25	582.0
26	1186.0
27	2349.0
28	3839.0
29	6044.0
30	9030.0
31	13019.0
32	19036.0
33	29895.0
34	62093.0
35	267138.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.46654248183073	20.130008010887085	13.131581233290543	23.271868273991643
2	15.53622643056832	19.974528008336286	39.96690570780056	24.522339853294834
3	18.976234180039413	25.51551302862849	29.22281692383022	26.285435867501878
4	12.31056448947989	15.894130785576971	36.90202538912219	34.89327933582095
5	13.137411448019353	38.45200163053834	32.32132218328119	16.08926473816111
6	30.610945350433084	37.84826827662228	16.997397409952555	14.543388962992085
7	28.478944105820975	32.569520993181165	18.84526046402806	20.106274436969805
8	28.764529519738918	32.948693519092494	17.785651744989593	20.50112521617899
9	28.03126967319284	13.99706213653588	19.81128207107314	38.160386119198144
10	17.535282100017607	26.868425686285384	29.507196318263716	26.089095895433285
11	36.0146362524633	23.83625149847921	20.483999739499794	19.665112509557705
12	23.949858533914473	25.764676654116027	28.27271477397989	22.012750037989615
13	29.353549435944686	21.511287136354976	25.998885637996366	23.13627778970397
14	23.245784358892102	20.1921430242627	27.319718177366877	29.242354439478323
15	26.131065373492174	26.847440947255947	24.133173495550995	22.888320183700888
16	24.327029765063436	27.836364513483524	23.82555839644942	24.011047325003616
17	22.576925952815692	26.79389368214633	25.75937016838444	24.86981019665354
18	22.05544312773917	26.336088685848807	28.928065761830446	22.680402424581573
19	24.711398841253683	24.46199341029364	29.093843014477095	21.73276473397558
20	26.04743044868857	23.96631820659646	27.78892678479254	22.19732455992243
21	27.543314189784052	22.725989961093813	26.993128100977597	22.73756774814454
22	25.700757621440136	24.197333732123777	27.21985976405435	22.882048882381746
23	24.161876759280922	25.034793662126404	28.840026339465542	21.963303239127132
24	22.867556074416946	24.89248232561393	28.73393265039811	23.506028949571014
25	23.63393843496886	25.070793514493978	27.835238044700013	23.460030005837147
26	23.735060361566383	24.64295620922127	28.288770698409255	23.333212730803094
27	24.292719637417054	25.027678547738557	28.073886647000222	22.605715167844163
28	23.359600944572687	25.224384254870603	28.076916582846696	23.339098217710013
29	23.14494323211516	25.408904851380786	28.49827416946667	22.947877747037385
30	22.952757234998142	25.70372085618768	28.180673545884773	23.16284836292941
31	23.263914014597837	25.792002547143873	27.95466276876468	22.98942066949361
32	23.51029958029813	24.867094408799268	27.982777750976894	23.639828259925707
33	23.300891252698484	25.474148847642823	28.2096554385711	23.015304461087595
34	23.528588829793648	24.822412774220005	28.571221824233874	23.077776571752477
35	23.408412247398612	25.173064213456314	28.124201010164356	23.294322528980718
36	23.207971325611574	25.230470879383287	28.005528406651454	23.556029388353682
37	23.76356794828501	25.209850933474844	27.300159197259877	23.726421920980272
38	23.3610798216056	25.538186194936163	27.892336228584107	23.208397754874127
39	24.16748274181955	25.27678213929076	27.752022499553767	22.80371261933592
40	24.29773316030932	25.07344676375951	27.381773624772666	23.247046451158504
41	24.153967128336493	25.61592157884327	27.300751117993542	22.929360174826694
42	24.278956902644406	25.818854580496076	26.542731919348338	23.359456597511176
43	23.87674124102997	25.296026050774888	27.218235542422963	23.608997165772177
44	23.449967677508372	25.73569850351688	27.749341489536196	23.064992329438553
45	24.431831886882566	25.81781956592004	26.71053456768087	23.03981397951652
46	24.367429048333484	25.900795507721103	26.76721871396939	22.964556729976024
47	23.630986982225686	26.297496062346383	26.742517252108726	23.328999703319205
48	23.812820358198156	26.28306096604957	26.477476934209733	23.426641741542547
49	23.94314218313562	26.33014776471496	25.953137889613053	23.773572162536365
50	23.393185359570452	26.962598111814906	25.80551790012977	23.83869862848487
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	165.0
1	106.5
2	48.0
3	98.0
4	148.0
5	179.0
6	210.0
7	188.5
8	167.0
9	221.5
10	276.0
11	505.5
12	735.0
13	1410.0
14	2085.0
15	2872.0
16	3659.0
17	3719.5
18	3780.0
19	3437.5
20	3095.0
21	2991.5
22	2888.0
23	2781.0
24	2674.0
25	2931.5
26	3189.0
27	3765.0
28	4341.0
29	5040.5
30	5740.0
31	6827.0
32	7914.0
33	9125.0
34	10336.0
35	11220.0
36	12104.0
37	13621.0
38	15138.0
39	15902.0
40	16666.0
41	18101.5
42	19537.0
43	21149.0
44	22761.0
45	25407.5
46	28054.0
47	30525.0
48	32996.0
49	34566.5
50	36137.0
51	37771.5
52	39406.0
53	38595.0
54	37784.0
55	34564.5
56	31345.0
57	27248.5
58	23152.0
59	19884.5
60	16617.0
61	13917.0
62	11217.0
63	9076.0
64	6935.0
65	6004.5
66	5074.0
67	3725.0
68	2376.0
69	2112.5
70	1849.0
71	1641.5
72	1434.0
73	1224.5
74	1015.0
75	849.5
76	684.0
77	521.0
78	358.0
79	303.0
80	248.0
81	169.5
82	91.0
83	79.0
84	67.0
85	52.5
86	38.0
87	32.0
88	26.0
89	17.5
90	9.0
91	5.0
92	1.0
93	4.0
94	7.0
95	5.5
96	4.0
97	4.5
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03642178843041388
2	0.0033768545564622143
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0016884272782311071
17	0.0
18	0.0
19	2.4120389689015817E-4
20	2.4120389689015817E-4
21	0.0
22	0.0
23	0.0
24	9.648155875606327E-4
25	2.4120389689015817E-4
26	4.8240779378031634E-4
27	0.001447223381340949
28	0.0019296311751212654
29	9.648155875606327E-4
30	0.001206019484450791
31	0.001206019484450791
32	0.0016884272782311071
33	4.8240779378031634E-4
34	4.8240779378031634E-4
35	2.4120389689015817E-4
36	2.4120389689015817E-4
37	0.0016884272782311071
38	0.0
39	2.4120389689015817E-4
40	2.4120389689015817E-4
41	0.001206019484450791
42	0.0033768545564622143
43	0.002894446762681898
44	0.0036180584533523725
45	0.0021708350720114236
46	0.0021708350720114236
47	0.0
48	0.002894446762681898
49	0.0021708350720114236
50	0.0021708350720114236
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	414587.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.992715204478856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.98701296166092	29.029478684166037
2	15.4199794170179	13.567335259035888
3	6.80081216401388	8.975585780718545
4	3.5854909753726703	6.30941933391196
5	2.2384068561128747	4.923679766636329
6	1.3693702251912232	3.6145388595798336
7	0.8915579594930958	2.7455438780186245
8	0.6688747794675325	2.3540494144459005
9	0.5006407326309956	1.9822090653357332
>10	2.3559356500553807	18.10753788087037
>50	0.1262402602643633	3.81092072727882
>100	0.05402421618299992	3.9387274743370853
>500	0.0011025350241428554	0.3448599512657678
>1k	5.512675120714277E-4	0.29611392439908757
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1221	0.2945099581028831	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	801	0.1932043214090167	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	621	0.1497876199687882	No Hit
CTTCAGACTCGCTTTCGCTTCGGCTCCGTCTTTTCTGACTTAACCTTGCA	477	0.11505425881660543	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	4.824077937803163E-4	0.0
23	0.0	0.0	0.0	9.648155875606326E-4	0.0
24	0.0	0.0	0.0	0.001447223381340949	0.0
25	0.0	0.0	0.0	0.0016884272782311071	0.0
26	0.0	0.0	0.0	0.0019296311751212652	0.0
27	0.0	0.0	0.0	0.0024120389689015814	0.0
28	0.0	0.0	0.0	0.0038592623502425303	0.0
29	0.0	0.0	0.0	0.00651250521603427	0.0
30	0.0	0.0	0.0	0.014231029916519332	0.0
31	0.0	0.0	0.0	0.02508520527657645	0.0
32	0.0	0.0	0.0	0.037386604017974515	0.0
33	0.0	0.0	0.0	0.0489643910687021	0.0
34	0.0	0.0	0.0	0.06367782877900176	0.0
35	0.0	0.0	0.0	0.08080330545820298	0.0
36	0.0	0.0	0.0	0.10516489904410896	0.0
37	0.0	0.0	0.0	0.14255150306208347	0.0
38	0.0	0.0	0.0	0.18645061229609225	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGA	25	0.0023513322	35.199036	11
CCTACAC	55	1.3703539E-7	31.999123	3
TACTGCG	30	0.0057414845	29.332531	5
CCGTTAT	55	1.5914522E-4	23.999342	9
GGTATCA	985	0.0	23.906471	1
GTATCAA	2060	0.0	23.396173	1
ATCCTAC	105	3.0431693E-9	23.05533	1
AGCGACC	50	0.002578016	21.999397	10
TTACGGC	80	3.6069214E-6	21.999397	18
AATACGT	50	0.002578016	21.999397	3
TAGGACC	90	4.0695159E-7	21.999395	4
GTAGCCC	115	8.731149E-9	21.042904	3
ATAGGAC	55	0.0044794395	19.999453	3
GGCCACG	55	0.0044794395	19.999453	11
ATTGGGC	55	0.0044794395	19.999453	34
ACTGGAT	55	0.0044794395	19.999453	3
TACGGCC	80	8.9723224E-5	19.249472	19
GGACCGC	80	8.9723224E-5	19.249472	6
CGCACAA	80	8.9723224E-5	19.249472	27
TCGGTCG	80	8.9723224E-5	19.249472	9
>>END_MODULE