FastQCFastQC Report
Thu 2 Feb 2017
SRR4062066_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062066_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198497
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20211.0181514078298413No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA18700.9420797291646725No Hit
GTCCTAAAGTGTGTATTTCTCATTT18380.9259585787190738No Hit
CTGTAGGACGTGGAATATGGCAAGA18290.9214245051562492No Hit
GTCCTACAGTGGACATTTCTAAATT16960.8544209736167298No Hit
CTTTAGGACGTGAAATATGGCGAGG15590.7854022982715104No Hit
TATCAACGCAGAGTACTTTTTTTTT13310.6705391013466199No Hit
GGTATCAACGCAGAGTACTTTTTTT8200.413104480168466No Hit
GTACTTTTTTTTTTTTTTTTTTTTT8000.40302876113996683No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7750.3904341123543429No Hit
GTCCTACAGTGTGCATTTCTCATTT7150.3602069552688454No Hit
ATTTAGAAATGTCCACTGTAGGACG7150.3602069552688454No Hit
GTCCTTCAGTGTGCATTTCTCATTT7050.3551690957545958No Hit
GAGTACTTTTTTTTTTTTTTTTTTT6630.3340100857947475No Hit
CTGAAGGACCTGGAATATGGCGAGA6130.30882078822349956No Hit
GATATACACTGTTCTACAAATCCCG6100.30730943036922476No Hit
TTGTAGAACAGTGTATATCAATGAG5700.2871579923122264No Hit
CTGTAGGACCTGGAATATGGCGAGA5610.28262391874940174No Hit
GTACATGGGGTGGTATCAACGCAAA5390.27154062781805266No Hit
GTGTATATCAATGAGTTACAATGAA5220.2629762666438284No Hit
GAATATGGCAAGAAAACTGAAAATC5040.2539081195181791No Hit
TTTCTAAATTTTCCACCTTTTTCAG4890.24635133024680472No Hit
GATATACACTGTTCTACAATGCCGG4070.20504088222995814No Hit
GTATCAACGCAGAGTACATGGGGTG4060.20453709627853317No Hit
GTGTATATCAATGAGTTACAATGAG4020.20252195247283333No Hit
ATTCCAGGTCCTTCAGTGTGCATTT3970.20000302271570855No Hit
GTTCTACAGTGTGGTTTTTATCATT3970.20000302271570855No Hit
GAAATATGGCGAGGAAAACTGAAAA3880.19546894915288393No Hit
GGAATATGGCGAGAAAACTGAAAAT3680.18539323012438474No Hit
GCCATATTCCACGTCCTACAGTGGA3630.18287430036725996No Hit
TTCCAGGTCCTTCAGTGTGCATTTC3570.1798515846587102No Hit
CCATATTCCAGGTCCTTCAGTGTGC3350.16876829372736113No Hit
CCATATTTCACGTCCTAAAGTGTGT3150.15869257469886194No Hit
GTTCTACAAATCCCGTTTCCAACGA3110.1566774308931621No Hit
TATCAACGCAGAGTACATGGGGTGG3100.15617364494173716No Hit
GACCTGGAATATGGCGAGAAAACTG3050.15365471518461238No Hit
ATACACACTTTAGGACGTGAAATAT3010.15163957137891254No Hit
GTCCACTGTAGGACGTGGAATATGG2920.1471054978160879No Hit
CTGTAGAACATATTAGATGAGTGAG2860.14408278210753814No Hit
CACTTTAGGACGTGAAATATGGCGA2810.14156385235041336No Hit
CTGTAGGACATGGAATATGGCAAGA2760.13904492259328857No Hit
GCAGAGTACTTTTTTTTTTTTTTTT2750.1385411366418636No Hit
ACCTGGAATATGGCGAGAAAACTGA2750.1385411366418636No Hit
CCACTGTAGGACGTGGAATATGGCA2720.13702977878758874No Hit
GTACATGGGTGGTATCAACGCAAAA2700.13602220688473882No Hit
ACAGTGGACATTTCTAAATTTTCCA2610.1314881333219142No Hit
GGTATCAACGCAGAGTACATGGGGT2530.12745784571051452No Hit
CCTAAAGTGTGTATTTCTCATTTTC2480.12493891595338973No Hit
GTATCAACGCAGAGTACATGGGAAA2460.12393134405053981No Hit
GTGTATTTCTCATTTTCCGTGATTT2430.12241998619626493No Hit
GAAATACACACTTTAGGACGTGAAA2430.12241998619626493No Hit
ATCAACGCAGAGTACTTTTTTTTTT2430.12241998619626493No Hit
ATGTTCTACAGTGTGGTTTTTATCA2430.12241998619626493No Hit
ACGCAGAGTACATGGGGTGGTATCA2280.11486319692489055No Hit
GTAGGACGTGGAATATGGCAAGAAA2260.11385562502204065No Hit
TCCTAAAGTGTGTATTTCTCATTTT2230.11234426716776576No Hit
GTCCTACAGTGGACATTTCTAAATA2210.11133669526491585No Hit
GTAGAACATATTAGATGAGTGAGTT2190.11032912336206592No Hit
CTACAGTGGACATTTCTAAATTTTC2160.10881776550779106No Hit
TATTAGATGAGTGAGTTACACTGAA2050.1032761200421165No Hit
AGTGTGTATTTCTCATTTTCCGTGA1990.10025340433356675No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGAGG250.006007927519.0032817
AGATTAT250.006016696318.9984875
CAATATT307.6944014E-418.9984864
TTCTGGA250.00602547618.9936942
TATGTCG350.002158732416.28852716
CTCTAGG551.1246464E-515.5363741
TCTAGGA508.677509E-515.1949562
TAGGACC1950.014.614224
GAAATGT2100.014.4750366
CCACCTT1450.014.41628313
AAGAGGG400.005242803614.2560618
TGTCGAG400.005242803614.2560618
CAAAAGA400.005251597614.25246115
ATATGTC400.005251597614.25246115
GAATATT400.005251597614.25246113
ATGTCGA400.005251597614.25246117
CCAAAAG400.005251597614.25246114
GCCAAAA400.005251597614.25246113
TAGAAAT2100.014.0226914
AAATGTC2100.014.0226917
Produced by FastQC (version 0.11.5)